Identification of Active Compounds of Pueraria Lobata and their Mechanisms of Action by LC-MS/MS and Network Pharmacology

Abstract

background: Recent studies have shown that osteoporosis has become a global public health problem and PL is a potential medicine for treatment of osteoporosis. objective: This study describes a rapid method of detecting and identifying the constituents and systematic mechanism of PL based on LC–MS and network pharmacology. method: We explored the compounds and possible pathways of PL affecting the pathological process of osteoporosis base on UHPLC-Q-Orbitrap MS metabolomics. result: A total of 48 compounds including 38 isoflavones, 6 puerosides and 4 others were identified by UHPLC-Q Exactive-Orbitrap MS. The network pharmacological analysis revealed that 28 compounds regulated 19 pathways through 27 targets, including estrogen signaling pathway and NF-kappa B signaling pathway, etc. conclusion: PL may attenuate osteoporosis by regulating estrogen signaling pathway, NF-kappa B signaling pathway, MAPK signaling pathway and PI3K-Akt signaling pathwayand be a potential source of functional food for prevention of osteoporosis.