Identification of active compounds and antioxidant activity of teak (Tectona grandis) leaves

Abstract

Abstract. Suryanti V, Kusumaningsih T, Marliyana SD, Setyono HA, Trisnawati EW. 2020. Identification of active compounds and antioxidant activity of teak (Tectona grandis) leaves. Biodiversitas 21: 941-947. Teak (Tectona grandis) grows in tropical area and the teak wood is widely used for building construction and furniture. Young teak leaf contains red pigment and often are used for natural food coloring. The research objectives were to isolate the young teak leaf chemical compounds and to investigate their antioxidant activities. The compounds identification was carried out by UV-Vis Spectroscopy, GC-MS and LC-MS. The antioxidant activity determination was carried out by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) method. The total phenolics content was determined using Folin-Ciocalteu method and total anthocyanins content was examined using the H-differential method. Two fractions were observed further by UV-Vis Spectroscopy, GC-MS and LC-MS. The UV-Vis spectrum of FA confirmed the presence of anthocyanins which has maximum absorbance at 208 and 492 nm. The GC-MS spectrum of fraction A (FA) revealed 4-hydroxy-4-methyl-2-pentanone, glycerin monoacetate, glycerin diacetate and 1-eicosanol. The UV-Vis spectrum of FC also confirms the presence of anthocyanins which has maximum absorbance at 489 nm. The LC-MS spectrum of fraction C (FC) shows anthocyanins, namely malvidin-3-o- (6-o-acetyl)-5-o-diglucoside. The FA and FC teak leaf fractions have strong antioxidant activities as their IC50 values are less than 50 ppm. Total phenolic of teak leaf extract was 6.17 mg GAE/g and total anthocyanins of teak leaf extract were 67.5 mg/L.

Bioactive Compound Contents and Antioxidant Activity of of Fermented Lead Tree (Leucaena leucocephala (lmk.) de Wit) Seeds

“…Phenolics are usually found in conjugated forms through hydroxyl groups with sugar and glycosides in plant materials (Metsa¨muuronen, & Sire´n, 2019;Bei et al, 2020). Phenolics compounds possess antioxidant activities (Suryanti, Kusumaningsih, Marliyana, Setyono and Trisnawati, 2020). During fermentation, several enzymes are produced, such as -amylase, -glycosidase and xylanase, and catalyse the release of the phenolics from the lead tree seeds substrate (Oliveira, Cipolatti, Furlong, & Soares, 2012;Leite, Silva, Salgado, & Belo, 2019) evidenced by the gradual increase observed for 2 days.…”

Actividad antioxidante e influencia del periodo climático sobre el contenido de polifenoles totales en Merremia aegyptia

“…Los resultados tabulados en el Cuadro 1 son similares a los obtenidos en trabajos previos [23], donde se reporta la inhibición del radical DPPH en un 60,31% utilizando extractos foliares de M. gangitica, haciendo énfasis en que la actividad antioxidante de los extractos fue superior a aquella obtenida con soluciones de ácido ascórbico a 100 µg mL -1 , lo cual fue relacionado con la concentración de polifenoles en los extractos. Así mismo, se ha obtenido correlación positiva alta (R 2 =0,907) entre la concentración de polifenoles en extractos foliares de I. batatas y la actividad antioxidante de los mismos [36], lo que coincide con otros reportes [39,54,55,56], donde se señala la relación entre un mayor contenido de compuestos fenólicos y la alta actividad antioxidante de extractos vegetales.…”

Antibacterial Activity of Chayote (Sechium edule Swartz) Squash Extracts and Their Phytochemical Constituents

“…Indonesia is a tropical country that is famous for its diversity of plant species. Studies have been conducted on many plants regarding their compounds and bioactivities (1)(2)(3)(4)(5). Indonesian people have used medicinal plants in an effort to overcome health problems.…”