Identification of ACE-inhibitory peptides in salt-free soy sauce that are transportable across caco-2 cell monolayers

Extraction of antioxidant and ACE inhibitory peptides from Thai traditional fermented shrimp pastes

“…In addition, the ACE inhibitory dipeptide Ile-Phe was also found in saltfree soy sauce possessing an IC 50 value of 65.8 µM (which was comparable with our results) and it was able to be transported across intestinal membranes through caco-2 cell monolayers (Zhu et al, 2008). Gobbetti, Minervini, & Rizzello (2004) revealed that di-and tri-peptides 15 with high ACE inhibitory activity had aromatic (such as Phe, Trp, Tyr), proline or hydrophobic amino acids (including Ile or Val) at C-terminus and a branched aliphatic residue at N-terminus (such as Ile or Val).…”

The (193–209) 17‐residues peptide of bovine β‐casein is transported through Caco‐2 monolayer

“…The hypothesis that peptides escape digestion and are transported from the intestinal lumen into blood circulation is gaining acceptance for small peptides, mainly due to the growing number of studies describing the in vitro transepithelial transport of bioactive peptides [13,[20][21][22][23][24][25][26][27][28][29]. In the present study we demonstrate that the 17 residues b-CN To exert its biological effects an ingested peptide must first resist intestinal hydrolysis.…”

Casein-derived bioactive peptides: Biological effects, industrial uses, safety aspects and regulatory status

“…Much research has been carried out on the transport of bioactive peptides in Caco-2 cells (Foltz et al, 2007;Iwan et al, 2008). Opioid peptides (Iwan et al, 2008;SienkiewiczSz1apka et al, 2009a), ACE inhibitors (Chua, Jois, Sim, & Go, 2004;Foltz et al, 2008;Zhu et al, 2008) and anti-hypertensive peptides (Quiró s, Dá valos, Lasunció n, Ramos, & Recio, 2008;Sun, Liu, Li, & Qin, 2009) have been shown to cross the epithelial cell monolayer. These studies found that the method of transport was not only dependent on the size of the peptide but also on charge, molecular weight, hydrogen bonding and hydrophobicity (Iwan et al, 2008;Pauletti, Okumu, & Burchardt, 1997;SienkiewiczSz1apka et al, 2009a).…”