Identification of Acarcidal Compounds in Inula japonica Extracts against Tetranychus cinnabarinus

Abstract

Abstract. In this study, we aimed to identify acaricidal compounds in Inula japonica against Tetranychus cinnabarinus. Here, I. japonica petroleum-ether extracts were found toxic to T. cinnabarinus with a LC50 value of 1.18 mg/ml. Silica gel column chromatography was used to further separate acaricidal components from I. japonica petroleum-ether extracts. Contact toxicity data showed that 17 out of 38 fractions groups had mite mortality rates above 50%, 9 fraction groups above 60 %, and fractions groups G5, G7 and G15 even above 80 % at a concentration of 2 mg/ml. The major vital compounds in the bioactive fraction groups were identified by GC/MS, and four compounds, namely β-Sitosterol (1), Stigmasterol (2), Lupeol (3), and α-Amyrin (4) were determined to have acaricidal activity to Tetranychus cinnabarinus.

Chemical variation of five natural extracts by non-polar solvent

“…The primary compound identified in Orthosiphon leaves in n-hexane, and petroleum ether extract is urs-12-en-3-one from the triterpenoid group (Figure 3). This compound also found in Inula japonica that used to be acaricidar compounds (Duan et al 2012). Acaricidar is pesticides that kill the member of arachnid subclass acari, which includes ticks and mites.…”

Effect of acaricidal components isolated from lettuce (Lactuca sativa) on carmine spider mite (Tetranychus cinnabarinus Boisd.)

“…Recently, phytogenic acaricides that are non-toxic to mammals and have low to no residual effects on the environment were found to be reasonable candidates for mite management (Benelli et al , 2016). Extracts of various plants such as Polygonum aviculare , Stellera chamaejasme , Inula japonica , Juglans regia , Albizzia julibrissin and Mentha piperita show acaricidal activities against mites (Wang et al , 2007 b , 2013; Ren et al , 2009 a ; Duan et al , 2012). These phytogenic acaricides have clear inhibitory effects on the growth and reproduction of mites through repellence, oviposition deterrence and growth regulation (Singh & Saratchandra, 2005).…”