Identification of absorbed components and their metabolites in rat plasma after oral administration of Shufeng Jiedu capsule using ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Abstract

Rationale: Shufeng Jiedu capsule (SFJDC), a prescription of traditional Chinese medicine, is mainly used for the treatment of acute upper respiratory tract infections. However, the bioactive components remain unclear, which partly limits its quality control and further development. This work aimed to carry out a study of plasma pharmacochemistry to identify the potential bioactive components of SFJDC.Methods: An effective approach based on a combination of ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/QTOF-MS) and multivariate statistical analysis was applied to comprehensively analyze the absorbed components and their metabolites in rat plasma after oral administration of SFJDC.After UPLC/QTOF-MS detection, the differences between control and dosed plasma samples were distinguished by multivariate statistical analysis, and chromatographic signals of xenobiotic compounds were further extracted to identify structures. Results:A total of 46 SFJDC-related xenobiotic compounds were identified as potential bioactive components in rat plasma. Among these, 27 absorbed prototype constituents were mainly flavonoids, anthraquinones, stilbenes, iridoids, lignans, naphthalenes, phenylethanoid glycosides and triterpenoid saponins. Especially for hastatoside, verbenalin, forsythoside A, phillyrin and emodin, they were closely related to the anti-inflammatory effect of SFJDC. Conclusions:The absorbed components and metabolites of SFJDC in rat plasma were analyzed for the first time. This study will be conducive for ascertaining the quality markers of SFJDC for quality control and pharmacological mechanism research at the molecular level.

The therapeutic effects of traditional chinese medicine on COVID-19: a narrative review

“…Shufeng Jiedu capsules (granules) are mainly used for acute upper respiratory tract infections. The granules consist of polygonum cuspidatum, forsythia, radix isatidis, bupleurum, radix, verbena, reed root, and licorice [ 21 ]. It has been found that Shufeng Jiedu has a broad-spectrum antiviral effect.…”

Simultaneous determination of multiple components in rat plasma by UHPLC-sMRM for pharmacokinetic studies after oral administration of Qingjin Yiqi Granules

“…For example, studies have shown that baicalin, baicalein, wogonoside and wogonin can be well absorbed into blood in the pharmacokinetics study of Scutellariae radix ( He, et al, 2018 ). Some researchers have studied the pharmacokinetics of verbenae herba , showing that verbenalin has a large plasma exposure ( Liu, et al, 2019 ). Finally, those compounds have potential activity, which is helpful to the further pharmacological studies of QJYQ ( Yang et al, 2017 ; Yuan et al, 2018 ).…”

Study on basic and clinical application of Shufeng Jiedu Capsule in treating respiratory tract infection

“…Furthermore, UPLC-Q-TOF–MS/MS was used to identify the absorption prototype components and their metabolites in rats plasma after oral administration of SFJDC [ 16 ]. A total of 46 exogenous compounds related to SFJDC were identified in rats plasma, including 27 absorption prototype drug components ( 8 flavonoids, 4 anthraquinones, 4 stilbenes, 2 iridoids, 2 lignans, 2 naphthalenes, 1 phenylethanoid glycoside, 1 triterpenoid saponin and 3 other compounds) and 19 metabolites.…”