Identification of ABF/AREB gene family in tomato (<i>Solanum lycopersicum</i> L.) and functional analysis of <i>ABF/AREB</i> in response to ABA and abiotic stresses

Phytohormones are very important for fruit development and ripening. However, it is unclear the role of phytohormones in pear fruit quality. In this study, gibberellin (GA), Indole-3-acetic acid (IAA), abscisic acid (ABA), and ethephon were selected to treat pear fruit at different stages,including 30, 45, 60, 75, and 90 days after flowering blooming (DAFB). As a result, exogenous GA treatment could promote fruit enlargement, decrease fruit firmness, and inhibit the accumulation of organ acids in ripening fruit (110DAFB). Exogenous IAA or ABA treatment could also promote fruit enlargement. Exogenous ABA or ethephon could promote the accumulation of soluble solids in ripening fruit (110DAFB), indicating the involvement of ABA and ethylene in fruit ripening. Quantitative real-time PCR (qPT-PCR) analysis suggested that PbZEP1, PbNCED.B, PbSDR4, and PbAO3 are the crucial genes for ABA biosynthesis, and PbACS1b and PbACO1 are the crucial genes for ethylene biosynthesis in pear fruit. EMSA and dual-luciferase assay suggested that PbABF.B and PbABF.C.2 directly bind to the PbACS1b promoter to enhance the activity, while PbABF.E.1 and PbABF.E.2 directly bind to the PbACO1promoter to enhance the activity. This result indicates that the four ABF proteins may be involved in ethylene biosynthesis during fruit ripening. Our study provides a foundation for the roles of GA, IAA, ABA and ethylene in pear fruit and reveals the ABA–ethylene cross-talking during fruit ripening.

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Identification and Characterization of the AREB/ABF/ABI5 Gene Family in Sandalwood (Santalum album L.) and Its Potential Role in Drought Stress and ABA Treatment

Liu,

Cheng,

Chen

et al. 2023

Forests

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AREB/ABF/ABI5 (ABA-responsive element-binding protein/ABRE binding factors and ABA INSENSITIVE 5) transcription factors are involved in regulating the expression of ABA (abscisic acid)-related genes and improving plant adaptability to environmental stress. To explore the influence of AREB/ABF transcription factors on santalol synthesis, we conducted a genome-wide analysis of the AREB gene family in sandalwood, identified 10 SaAREB genes, and divided them into five subfamilies. We found that all SaAREB genes encoded unstable hydrophilic proteins and the subcellular localization prediction of SaAREBs was that they are located in the nucleus. AREB/ABF genes belong to the bZIP-A subfamily and we found that the 10 AREB proteins all contained bZIP (basic region leucine zipper) and four potential phosphorylation sites (RXXS/T). According to the collinearity analysis results, four of the SaAREB genes were involved in two fragment duplication events. Through qRT-PCR (real-time fluorescence quantitative PCR), we explored the expression profile of SaAREB in different tissues; the effects of ABA treatment and drought treatment on AREB transcription factors were predicted. From the expression of different tissues, we found that SaAREB1 not only responded to prolonged drought but also was highly expressed in stems. Moreover, SaAREB3, SaAREB7, and SaAREB8 specifically respond to ABA treatment. Based on RNA-seq (RNA sequencing) data, we found that SaAREB6 and SaAREB8 were highly expressed in the sapwood and transition regions. Regarding SaCYP736A167, as a key gene in santalol synthesis, its promoter contains the most ABRE cis-reactive elements. These results provide a basis for further analysis of the role of the Santalum album L. (S. album) ABRE/ABF/ABI5 genes in the formation of santalols.

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Identification of ABF/AREB gene family in tomato (Solanum lycopersicum L.) and functional analysis of ABF/AREB in response to ABA and abiotic stresses

Cited by 6 publications

References 54 publications

Integrated transcriptomic and metabolomic analyses revealed the role of SlMYC2 in tomato (Solanum lycopersicum L.) fruit development and ripening

Integrated transcriptomic and metabolomic analyses revealed the role of SlMYC2 in tomato (Solanum lycopersicum L.) fruit development and ripening

Effects of exogenous GA, IAA, ABA and ethylene on pear fruit during different development stages

Identification and Characterization of the AREB/ABF/ABI5 Gene Family in Sandalwood (Santalum album L.) and Its Potential Role in Drought Stress and ABA Treatment

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