Identification of aberrantly expressed glycans in gastric cancer by integrated lectin microarray and mass spectrometric analyses

Li, Xiang; Guan, Feng; Li, Dongliang; Tan, Zengqi; Yang, Ganglong; Wu, Yanli; Huang, Zhaohui

doi:10.18632/oncotarget.13539

Cited by 12 publications

(8 citation statements)

References 46 publications

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“…It has been well documented that Tn antigen is one of the most expressed tumour antigen found in many human cancers, such as colon, breast, cervix, stomach and skin . Many studies have established an association of Tn antigen expression with cancer progression and metastasis and poor outcome .…”

Section: Discussionmentioning

confidence: 99%

Tn antigen promotes human colorectal cancer metastasis via H‐Ras mediated epithelial‐mesenchymal transition activation

Liu

Dong

et al. 2019

J Cellular Molecular Medi

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Tn antigen is a truncated O‐glycan, frequently detected in colorectal cancer ( CRC ), but its precise role in CRC metastasis is not well addressed. Here we investigated the effects of Core 1 β3Gal‐T specific molecular chaperone (Cosmc) deletion‐mediated Tn antigen exposure on CRC metastasis and its underlying mechanism. We first used CRISPR /Cas9 technology to knockout Cosmc, which is required for normal O‐glycosylation, and thereby obtained Tn‐positive CRC cells. We then investigated the biological consequences of Tn antigen expression in CRC . The results showed that Tn‐positive cells exhibited an enhanced metastatic capability both in vitro and in vivo. A further analysis indicated that Tn antigen expression induced typical activation of epithelial‐mesenchymal transition ( EMT ). Mechanistically, we found that H‐Ras, which is known to drive EMT , was markedly up‐regulated in Tn‐positive cells, whereas knockdown of H‐Ras suppressed Tn antigen induced activation of EMT . Furthermore, we confirmed that LS 174T cells (Tn‐positive) transfected with wild‐type Cosmc, thus expressing no Tn antigen, had down‐regulation of H‐Ras expression and subsequent inhibition of EMT process. In addition, analysis of 438 samples in TCGA cohort demonstrated that Cosmc expression was reversely correlated with H‐Ras, underscoring the significance of Tn antigen‐H‐Ras signalling in CRC patients. These data demonstrated that Cosmc deletion‐mediated Tn antigen exposure promotes CRC metastasis, which is possibly mediated by H‐Ras‐induced EMT activation.

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Section: Discussionmentioning

confidence: 99%

Tn antigen promotes human colorectal cancer metastasis via H‐Ras mediated epithelial‐mesenchymal transition activation

Liu

Dong

et al. 2019

J Cellular Molecular Medi

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“…All four lectins showed binding specificity to high-mannose glycan structures, thereby the glycans were significantly increased in moderately-differentiated components compared with well-differentiated components in HCC. NPA-one of the elevated lectins that binds high-mannose glycans-has been reported to be increased in gastric cancer cell lines [32]. Therefore, we performed NPA staining to confirm elevated high-mannose glycan expression and demonstrated that increased high-mannose glycans expression was associated with decreased MGAT1 expression.…”

Section: Discussionmentioning

confidence: 93%

Altered glycosylation associated with dedifferentiation of hepatocellular carcinoma: a lectin microarray-based study

et al. 2020

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Background: Altered glycosylation associated with hepatocellular carcinoma (HCC) is well documented. However, few reports have investigated the association between dedifferentiation and glycosylation. Therefore, the aim of this study was to analyze glycosylation associated with dedifferentiation of HCC within the same nodule and to investigate glycosyltransferase related to the glycosylation. Methods: We analyzed resected HCC specimens (n = 50) using lectin microarray to comprehensively and sensitively analyze glycan profiles, and identify changes to glycosylation between well-and moderately-differentiated components within the same nodule. Moreover, we performed immunohistochemical staining of mannosyl(α-1,3-)-glycoprotein β-1,2-N-acetylglucosaminyltransferase (MGAT1), which is an essential glycosyltransferase that converts high-mannose glycans to complex-or hybrid-type N-glycans. Results: Four lectins from Narcissus pseudonarcissus agglutinin (NPA), Concanavalin A, Galanthus nivalis agglutinin, and Calystegia sepium agglutinin were significantly elevated in moderately-differentiated components of HCC compared with well-differentiated components, and all lectins showed binding specificity to high-mannose glycans. Therefore, these structures were represented to a greater extent in moderately-differentiated components than in well-differentiated ones. Immunohistochemical staining revealed significantly increased NPA expression and decreased MGAT1 expression in moderately-differentiated components. Low MGAT1 expression in moderatelydifferentiated components of tumors was associated with intrahepatic metastasis and had tendency for poor prognosis. Conclusion: Dedifferentiation of well-differentiated HCC is associated with an increase in high-mannose glycans. MGAT1 may play a role in the dedifferentiation of HCC.

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“…A lectin/glyco-antibody microarray performed on sera from 22 pancreatic cancer sera, discriminated cancer patients from other diseases (35 chronic pancreatitis, 37 diabetes) and normal controls (89 controls), by a 70% increase inthe response of serum α1β-glycoprotein to SNA ( Sambucus nigra agglutinin) [121]. Using a 45-immobilized lectin microarray, the cell surface glycan changes occurring during the malignant transformation of endometrium, were investigated [122].…”

Section: Tn/t-specific Lectins For Cancer Diagnosis/prognosismentioning

confidence: 99%

Plant Lectins Targeting O-Glycans at the Cell Surface as Tools for Cancer Diagnosis, Prognosis and Therapy

Poiroux

Barre

Damme

et al. 2017

IJMS

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Aberrant O-glycans expressed at the surface of cancer cells consist of membrane-tethered glycoproteins (T and Tn antigens) and glycolipids (Lewis a, Lewis x and Forssman antigens). All of these O-glycans have been identified as glyco-markers of interest for the diagnosis and the prognosis of cancer diseases. These epitopes are specifically detected using T/Tn-specific lectins isolated from various plants such as jacalin from Artocarpus integrifola, and fungi such as the Agaricus bisporus lectin. These lectins accommodate T/Tn antigens at the monosaccharide-binding site; residues located in the surrounding extended binding-site of the lectins often participate in the binding of more extended epitopes. Depending on the shape and size of the extended carbohydrate-binding site, their fine sugar-binding specificity towards complex O-glycans readily differs from one lectin to another, resulting in a great diversity in their sugar-recognition capacity. T/Tn-specific lectins have been extensively used for the histochemical detection of cancer cells in biopsies and for the follow up of the cancer progression and evolution. T/Tn-specific lectins also induce a caspase-dependent apoptosis in cancer cells, often associated with a more or less severe inhibition of proliferation. Moreover, they provide another potential source of molecules adapted to the building of photosensitizer-conjugates allowing a specific targeting to cancer cells, for the photodynamic treatment of tumors.

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Identification of aberrantly expressed glycans in gastric cancer by integrated lectin microarray and mass spectrometric analyses

Cited by 12 publications

References 46 publications

Tn antigen promotes human colorectal cancer metastasis via H‐Ras mediated epithelial‐mesenchymal transition activation

Tn antigen promotes human colorectal cancer metastasis via H‐Ras mediated epithelial‐mesenchymal transition activation

Altered glycosylation associated with dedifferentiation of hepatocellular carcinoma: a lectin microarray-based study

Plant Lectins Targeting O-Glycans at the Cell Surface as Tools for Cancer Diagnosis, Prognosis and Therapy

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