Identification of a Zika NS2B epitope as a biomarker for severe clinical phenotypes

Loeffler, Felix F.; Viana, Isabelle Freire Tabosa; Fischer, Nico; Coêlho, Danilo F.; Silva, Carolina S.; Purificacao, Antonio; Araújo, Catarina M C S; Leite, Bruno; Durães-Carvalho, Ricardo; Magalhães, Tereza; Morais, Clarice Neuenschwander Lins de; Cordeiro, Marli Tenório; Lins, Roberto D.; Marques, Ernesto T. A.; Jaenisch, Thomas

doi:10.1039/d1md00124h

Cited by 3 publications

(1 citation statement)

References 70 publications

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“…The affinity of the anti-Gag IgG antibodies was quantified using a microscale thermophoresis assay (MST), as previously described [19]. The p24 HIV protein (20 µM) was labeled using a RED fluorescent dye NT-647-NHS Labeling Kit (NanoTemper Technologies, Munich, Germany), according to the manufacturer's instructions.…”

Section: Anti-p24 Antibody Affinity By Microscale Thermophoresismentioning

confidence: 99%

LAMP-1 Chimeric to HIV-1 p55Gag in the Immunization of Neonate Mice Induces an Early Germinal Center Formation and AID Expression

et al. 2022

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Neonates have a limited adaptive response of plasma cells, germinal center (GC) B cells, and T follicular helper cells (TFH). As neonatal vaccination can be an important tool for AIDS prevention, these limitations need to be overcome. Chimeric DNA vaccine encoding p55Gag HIV-1 protein conjugated with lysosomal-associated membrane protein 1 (LAMP-1) has been described as immunogenic in the neonate period. Herein, we investigated the immunologic mechanisms involved in neonatal immunization with a LAMP-1/p55Gag (LAMP/Gag) DNA vaccine in a C57BL/6 mouse background. Neonatal LAMP/Gag vaccination induced strong Gag-specific T-cell response until adulthood and elevated levels of anti-Gag IgG antibodies. We also demonstrated for the first time that the immunogenicity of the neonatal period with LAMP/Gag is due to the induction of high-affinity anti-p24 IgG antibodies and long-term plasma cells. Together with that, there is the generation of early TFH cells and the formation of GC sites with the upregulation of activation-induced cytidine deaminase (AID) enzyme mRNA and protein expression in draining lymph nodes after neonatal LAMP/Gag vaccination. These findings underscore that the LAMP-1 strategy in the chimeric vaccine could be useful to enhance antibody production even in the face of neonatal immaturity, and they contribute to the development of new vaccine approaches for other emerging pathogens at an early stage of life.

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Section: Anti-p24 Antibody Affinity By Microscale Thermophoresismentioning

confidence: 99%

LAMP-1 Chimeric to HIV-1 p55Gag in the Immunization of Neonate Mice Induces an Early Germinal Center Formation and AID Expression

et al. 2022

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Machine learning-based gene expression biomarkers to distinguish Zika and Dengue virus infections: implications for diagnosis

Zeba,

Rajalingam,

Sekar

et al. 2024

VirusDis.

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Are Zika virus cross-reactive antibodies against aquaporin-4 associated to Neuromyelitis Optica Spectrum Disorder?

Brainer-Lima

Leite

Araújo

et al. 2021

Journal of Neuroimmunology

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Identification of a Zika NS2B epitope as a biomarker for severe clinical phenotypes

Abstract: The identification of specific biomarkers for Zika infection and its clinical complications is fundamental to mitigate the infection spread, which has been associated with a broad range of neurological sequelae.

Cited by 3 publications

References 70 publications

LAMP-1 Chimeric to HIV-1 p55Gag in the Immunization of Neonate Mice Induces an Early Germinal Center Formation and AID Expression

LAMP-1 Chimeric to HIV-1 p55Gag in the Immunization of Neonate Mice Induces an Early Germinal Center Formation and AID Expression

Machine learning-based gene expression biomarkers to distinguish Zika and Dengue virus infections: implications for diagnosis

Are Zika virus cross-reactive antibodies against aquaporin-4 associated to Neuromyelitis Optica Spectrum Disorder?

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