Identification of a Universally Primed-PCR-Derived Sequence-Characterized Amplified Region Marker for an Antagonistic Strain of
            <i>Clonostachys rosea</i>
            and Development of a Strain-Specific PCR Detection Assay

Булат, С. А.; Lübeck, Mette; Alekhina, Irina A.; Jensen, Dan Funck; Knudsen, Inge M.B.; Lübeck, Peter Stephensen

doi:10.1128/aem.66.11.4758-4763.2000

Cited by 67 publications

(66 citation statements)

References 31 publications

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“…Random amplified polymorphic DNA (RAPD) has been used in many studies. It is based on the use of short general primers that anneal to unspecified regions in the template DNA whereas universally primed (UP) PCR is based on longer general primers and a higher annealing temperature which makes it more robust in terms of reproducibility (Bulat et al, 1998;Bulat et al, 2000;Lubeck et al, 1999;Sabu et al, 2011). UP-PCR has been used to separate sympatric isolates of Beauveria in Denmark and was used to place isolates in genetic groups (Meyling and Eilenberg, 2006).…”

Section: Elucidation On Molecular Studies Of Entomopathogenic Fungimentioning

confidence: 99%

A Review on Prospects of Entomopathogenic Fungi as Potent Biological Control Agents of Insect Pests

Devi¹,

Devi²,

Deepshikha³

2016

Int. J. Curr. Res. Biosci. Plant Biol.

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A b s t r a c t A r t i c l e I n f oInsecticide resistance and the demand for reduced chemical inputs in agriculture have provided a movement to the development of alternative measures of controlling insect pests. Myco-biocontrol offers an attractive alternative to the use of chemical pesticides.Entomopathogenic fungi are potential biological control agents mainly by reason of their wide host range, high reproductive capabilities, target specific activity, short generation time, and resting stage or saprobic phase producing capabilities that can ensure their survival for a longer time when no host is present. However, a primary requirement for the commercial use of entomopathogenic fungus as myco-biocontrol agents is the susceptibility of the insect and also the virulence of the fungus. They are naturally occurring organisms which are perceived as less damaging to the environment. The current paper could enlightened regarding the recent progress in the field of entomopathogenic fungi and their possible mode of action along with further improvement aspects for perceptive of myco-biological control of insect pests.

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Section: Elucidation On Molecular Studies Of Entomopathogenic Fungimentioning

confidence: 99%

A Review on Prospects of Entomopathogenic Fungi as Potent Biological Control Agents of Insect Pests

Devi¹,

Devi²,

Deepshikha³

2016

Int. J. Curr. Res. Biosci. Plant Biol.

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“…The genome structure of the three isolates and their closest phylogenetic neighbours was examined using universally primed PCRs with primers AS4/AA2M2 (Bulat et al, 2000). The set-up and protocol were as described previously (Carlsohn et al, 2007).…”

Section: The Genus Kribbellamentioning

confidence: 99%

Kribbella aluminosa sp. nov., isolated from a medieval alum slate mine

Carlsohn

Groth

Spröer

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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Three actinomycetes (strains HKI 0478 T , HKI 0479 and HKI 0480) isolated from the surfaces of rocks in the Feengrotten medieval alum slate mine (Thuringia, Germany) were examined in a polyphasic taxonomic study. The following morphological and chemotaxonomic features supported their classification as members of the genus Kribbella: the presence of LLdiaminopimelic acid in the cell-wall peptidoglycan; glucose together with minor amounts of mannose and ribose as the whole-cell sugars; polar lipids comprising phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unknown phospho-and glycolipids; fatty acid profiles characterized by the predominance of anteiso-C 15 : 0 , iso-C 16 : 0 and C 16 : 0 9-methyl; and the presence of MK-9(H 4 ) as the main menaquinone. The isolates had almost identical 16S rRNA gene sequences (99.9-100 %) and were most closely related to the type strains of Kribbella jejuensis (98.9 % sequence similarity), Kribbella swartbergensis and Kribbella solani (both 98.8 %). A wide range of genotypic and phenotypic markers as well as the low levels of DNA-DNA relatedness between strain HKI 0478 T and the type strains of K. jejuensis (41.3 %), K. swartbergensis (18.6 %) and K. solani (14.2 %) distinguished the novel strains from their closest phylogenetic neighbours. On the basis of these results, strain HKI 0478 T represents a novel member of the genus Kribbella, for which the name Kribbella aluminosa sp. nov. is proposed. The type strain is HKI 0478 T (5DSM 18824 T 5JCM 14599 T ). Park et al. 1999 emend. Sohn et al. 2003 is a member of the family Nocardioidaceae, which was proposed by Nesterenko et al. (1985Nesterenko et al. ( , 1990 and the description of which was emended by Rainey et al. (in Stackebrandt et al., 1997). The genus Kribbella is represented by Gram-positive or Gram-variable, nonmotile actinomycetes that form an extensively branched vegetative mycelium and aerial hyphae that fragment into short to elongated rod-like or coccoid elements. Strains of the 11 Kribbella species with validly published names share the following chemotaxonomic characteristics (Park et al., 1999;Sohn et al., 2003): the presence of LL-diaminopimelic acid in the peptidoglycan (wall chemotype I sensu Lechevalier & Lechevalier, 1970), fatty acids that consist mainly of anteiso-and iso-branched components, MK-9(H 4 ) as the main menaquinone and polar lipids comprising phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol (phospholipid pattern III sensu Lechevalier et al., 1977). A combination of these chemotaxonomic markers serves to separate the strains of the genus Kribbella from members of the other genera within the family Nocardioidaceae. The genus KribbellaThree filamentously growing actinomycetes, strains HKI 0478 T , HKI 0479 and HKI 0480, were isolated from acidic and heavy-metal-containing rock surfaces in a small mining area behind the 'Märchendom', the third level of the Feengrotten medieval alum slate mine (Saalfeld, Thuringia,...

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“…Universally primed PCR (UP-PCR) is a DNA fingerprinting technique closely related to the random amplified polymorphic DNA (RAPD) method (Bulat et al 1998). UP-PCR has the ability to generate numerous bands from primarily intergenic, less conserved regions of the genome (Bulat et al 1998).…”

Section: Introductionmentioning

confidence: 99%

“…UP-PCR has the ability to generate numerous bands from primarily intergenic, less conserved regions of the genome (Bulat et al 1998). UP primers are longer (15-21 nt) than RAPD primers (typically 10 nt) and therefore can anneal under more stringent conditions (52-60 C) ensuring greater reproducibility of banding profiles.…”

Section: Introductionmentioning

confidence: 99%

Development of SCAR markers and UP-PCR cross-hybridization method for specific detection of four major subgroups of Rhizoctonia from infected turfgrasses

et al. 2014

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A rapid identification assay for Waitea circinata (anamorph: Rhizoctonia spp.) varieties zeae and circinata causing patch diseases on turfgrasses was developed based on the universally primed PCR (UP-PCR) products cross-blot hybridization. Tester isolates belonging to the two varieties of W. circinata were amplified with a single UP primer L21, which generated multiple DNA fragments for each variety. Probes were prepared with UP-PCR products of each tester isolate by labeling with digoxigenin. Fieldcollected W. circinata isolates and representative isolates of different R. solani anastomosis groups (AG) and AG subgroups were amplified with L21, immobilized on nylon membrane and cross hybridized with the two probes. Isolates within a W. circinata variety cross-hybridized strongly, while non-homologous isolates did not cross-hybridize or did so weakly. Closely related W. circinata varieties zeae and circinata were clearly distinguished with this assay. Sequencecharacterized amplified region (SCAR) markers also were developed from UP-PCR products to identify isolates of Thanatephorus cucumeris (anamorph: R. solani) AG 1-IB and AG 2-2IIIB. These two AGs are commonly isolated from diseased, cool-season turfgrasses. The specific SCAR markers that were developed could differentiate isolates of AG 1-IB or AG 2-2IIIB groups. These SCAR markers did not amplify a product from genomic DNA of nontarget isolates of Rhizoctonia. The specificities and sensitivities of the SCAR primers were tested on total DNA extracted from several field-grown, cool-season turf species having severe brown-patch symptoms. First, the leaf samples from diseased turf species were tested for the anastomosis groups of the causal pathogen, and thereafter the total DNA was amplified with the specific primers. The specific primers were sensitive and unique enough to produce a band from total DNA of diseased turfgrasses infected with either AG 1-IB or AG 2-2IIIB.

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Identification of a Universally Primed-PCR-Derived Sequence-Characterized Amplified Region Marker for an Antagonistic Strain of Clonostachys rosea and Development of a Strain-Specific PCR Detection Assay

Cited by 67 publications

References 31 publications

A Review on Prospects of Entomopathogenic Fungi as Potent Biological Control Agents of Insect Pests

A Review on Prospects of Entomopathogenic Fungi as Potent Biological Control Agents of Insect Pests

Kribbella aluminosa sp. nov., isolated from a medieval alum slate mine

Development of SCAR markers and UP-PCR cross-hybridization method for specific detection of four major subgroups of Rhizoctonia from infected turfgrasses

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