Identification of a Unique Monocarboxylate Transporter (MCT3) in Retinal Pigment Epithelium

Yoon, Heeyong; Fanelli, Albertina; Grollman, Evelyn F.; Philp, Nancy J.

doi:10.1006/bbrc.1997.6588

Cited by 136 publications

(101 citation statements)

References 17 publications

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“…4). Our observation is similar to what has been described in RPE in situ (6,10,14,25,26). Furthermore, the human RPE cell line ARPE-19 expresses CD147 and MCT1 at the apical membrane and MCT4 and CD147 at the basolateral membrane (ref.…”

Section: Mechanisms Underlying Mct Polarity In Rpesupporting

confidence: 90%

See 1 more Smart Citation

Mechanisms regulating tissue-specific polarity of monocarboxylate transporters and their chaperone CD147 in kidney and retinal epithelia

Deora

Philp²,

Hu³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

126

121

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Proton-coupled monocarboxylate transporters (MCT) MCT1, MCT3, and MCT4 form heterodimeric complexes with the cell surface glycoprotein CD147 and exhibit tissue-specific polarized distributions that are essential for maintaining lactate and pH homeostasis. In the parenchymal epithelia of kidney, thyroid, and liver, MCT͞CD147 heterocomplexes are localized in the basolateral membrane where they transport lactate out of or into the cell depending on metabolic conditions. A unique distribution of lactate transporters is found in the retinal pigment epithelium (RPE), which regulates lactate levels of the outer retina. In RPE, MCT1͞ CD147 is polarized to the apical membrane and MCT3͞CD147 to the basolateral membrane. The mechanisms responsible for tissuespecific polarized distribution of MCTs are unknown. Here, we demonstrate that CD147 carries sorting information for polarized targeting of the MCT1͞CD147 hetero-complexes in kidney and RPE cells. In contrast, MCT3 and MCT4 harbor dominant sorting information that cotargets CD147 to the basolateral membrane in both epithelia. RNA interference experiments show that MCT1 promotes CD147 maturation. Our results open a unique paradigm to study the molecular basis of tissue-specific polarity.apical ͉ basolateral ͉ polarized epithelium

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Section: Mechanisms Underlying Mct Polarity In Rpesupporting

confidence: 90%

“…Monocarboxylate transporters (MCTs) are nonglycosylated multispan proteins with 12 predicted transmembrane domains and cytoplasmic N-and C-terminal ends (6,(10)(11)(12)(13)(14). Of 14 known family members, only MCT1-MCT4 are well characterized; they share 40-60% homology and have distinct tissue distribution and lactate͞ proton transport kinetics (15,16).…”

mentioning

confidence: 99%

Mechanisms regulating tissue-specific polarity of monocarboxylate transporters and their chaperone CD147 in kidney and retinal epithelia

Deora

Philp²,

Hu³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

126

121

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“…This is significantly greater than the 43 % and 45 % sequence identity that it shares with MCT1 and MCT2 respectively [16]. However, this degree of identity is lower than might be expected between species.…”

Section: Sequence Analysis Of New Mctsmentioning

confidence: 70%

“…A cDNA has been identified in a chicken retinal cDNA library that encodes a developmentally regulated integral membrane protein of 50 kDa that seems to be closely related to MCT1 and MCT2. The protein was shown to be expressed in chick retinal epithelia and was initially named REMP (retinal epithelial membrane protein) [15] and subsequently functionally expressed and renamed MCT3 [16]. Hydrophobicity plots again predict 12 TM domains similar to MCT1 and MCT2, with which it shares 43 % and 45 % identity respectively [16].…”

Section: Introductionmentioning

confidence: 99%

Cloning and sequencing of four new mammalian monocarboxylate transporter (MCT) homologues confirms the existence of a transporter family with an ancient past

Price

Jackson

Halestrap

1998

Biochemical Journal

331

319

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Measurement of monocarboxylate transport kinetics in a range of cell types has provided strong circumstantial evidence for a family of monocarboxylate transporters (MCTs). Two mammalian MCT isoforms (MCT1 and MCT2) and a chicken isoform (REMP or MCT3) have already been cloned, sequenced and expressed, and another MCT-like sequence (XPCT) has been identified. Here we report the identification of new human MCT homologues in the database of expression sequence tags and the cloning and sequencing of four new full-length MCT-like sequences from human cDNA libraries, which we have denoted MCT3, MCT4, MCT5 and MCT6. Northern blotting revealed a unique tissue distribution for the expression of mRNA for each of the seven putative MCT isoforms (MCT1-MCT6 and XPCT).

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“…Their expression in the CNS appears to be preferentially associated with (but not always restricted to) a particular cell type, often correlating with their main metabolic phenotype. Thus, MCT1, MCT2, and MCT4 are predominantly expressed, respectively, in oligodendrocytes, neurons, and astrocytes (Lee et al, 2012), while MCT3 is found only in retinal pigment epithelial cells (Yoon et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Distribution of Monocarboxylate Transporters in the Peripheral Nervous System Suggests Putative Roles in Lactate Shuttling and Myelination

et al. 2015

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Identification of a Unique Monocarboxylate Transporter (MCT3) in Retinal Pigment Epithelium

Cited by 136 publications

References 17 publications

Mechanisms regulating tissue-specific polarity of monocarboxylate transporters and their chaperone CD147 in kidney and retinal epithelia

Mechanisms regulating tissue-specific polarity of monocarboxylate transporters and their chaperone CD147 in kidney and retinal epithelia

Cloning and sequencing of four new mammalian monocarboxylate transporter (MCT) homologues confirms the existence of a transporter family with an ancient past

Distribution of Monocarboxylate Transporters in the Peripheral Nervous System Suggests Putative Roles in Lactate Shuttling and Myelination

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