Identification of a third genomic group of Borrelia burgdorferi through signature nucleotide analysis and 16S rRNA sequence determination

Marconi, Richard T.; Garon, Claude F.

doi:10.1099/00221287-138-3-533

Cited by 65 publications

(63 citation statements)

References 19 publications

(13 reference statements)

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“…These length differences lie to the right of the rightmost mapped cleavage site (the highly conserved SgrAI no. 2, which is 32 kbp from the right end in Sh-2-82), and these strains have no extra right-end SgrAI restriction sites relative to JD1 and Sh-2-82 that could explain the fragment length differences (46a (4), and FujiP1 and G2 belong to this group according to the analyses of I. Schwartz (63a) and T. Masuzawa (41a) and Marconi and Garon (38,39), respectively. VS461 typifies B. afzelii (13).…”

Section: Resultsmentioning

confidence: 99%

“…VS461 typifies B. afzelii (13). IPF belongs to this group by the analysis of Marconi and Garon (38), and R-IP3 is included in this group by the analyses of Marconi and Garon (38), Schwartz et al (64), and Baranton et al (4). HO14 is the type strain for B. japonica, and IKA2 is included in this group by the analysis of Kawabata et al (28).…”

Section: Resultsmentioning

confidence: 99%

“…We also analyzed B. burgdorferi isolate 1352, which was reported to have been isolated from an Amblyomma americanum tick in Texas (38)(39)(40)56). We found this isolate to be identical to high-passage strain B31 in its plasmid pattern and in its restriction site cleavage map.…”

Section: Methodsmentioning

confidence: 99%

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Linear chromosomes of Lyme disease agent spirochetes: genetic diversity and conservation of gene order

et al. 1995

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We have constructed physical and genetic maps of the chromosomes of 21 Lyme disease agent spirochetes from geographically diverse locations. All have linear chromosomes whose lengths range from 935 to 955 kbp, and all contain multiple linear plasmids in the 16-to 175-kbp size range. The locations of 11 gene clusters on the chromosomes of these different isolates are indistinguishable at the resolution achieved in this study, indicating that the members of this related group of species have highly conserved chromosomal gene orders. However, chromosomal restriction endonuclease cleavage site maps are unique for nearly all isolates. The 22 chromosomal maps currently available define eight classes of Lyme disease agents. Four of these correspond to the previously proposed species Borrelia burgdorferi, Borrelia garinii, Borrelia afzelii, and Borrelia japonica. In addition, the North American isolates 21038, DN127 cl9-2, 25015, and CA55 typify four additional chromosomal types that are as phylogenetically distinct as the species listed above. These findings support the idea that comparison of restriction maps is currently the most robust and definitive method for determining overall chromosomal relationships among closely related bacteria. In the course of this work, we located on the chromosome the previously unmapped outer surface protein-encoding LA7 gene and genes homologous to the Escherichia coli priA, plsC, parE, and parC genes, and we have substantially refined the locations of the recA, fla, p22A, and flgE genes.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Linear chromosomes of Lyme disease agent spirochetes: genetic diversity and conservation of gene order

et al. 1995

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“…The phylogenetic diversity of the Lyme disease spirochetes is also an important factor to consider in vaccine and diagnostic assay development and has only recently been appreciated. Borrelia burgdorferi, originally identified as the single causative agent of Lyme disease (8), has been subdivided into several Borrelia species, including B. burgdorferi, B. garinii, B. afzelii (2,7,22,23,33,42), B. japonica (18,25,32), and B. andersonii sp. nov. (25).…”

mentioning

confidence: 99%

Molecular and evolutionary analyses of a variable series of genes in Borrelia burgdorferi that are related to ospE and ospF, constitute a gene family, and share a common upstream homology box

et al. 1996

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In this study we report on the molecular characterization of a series of genes that constitute a gene family related to ospE and ospF. Some members of this family appear to represent recombined or variant forms of ospE and ospF. Variant ospE and ospF genes were found in several Borrelia burgdorferi isolates, demonstrating that their occurrence is not a phenomenon relevant to only a single isolate. Hybridization analyses revealed that the upstream sequence originally identified 5 of the full-length ospEF operon exists in multiple copies ranging in number from two to six depending on the isolate. This repeated sequence, which we refer to as the upstream homology box (UHB), carries a putative promoter element. In some isolates, UHB elements were found to flank copies of ospE and ospF that exist independently of each other. We refer to this group of UHB-flanked genes collectively as the UHB gene family. The evolutionary relationships among UHB gene family members were assessed through DNA sequence analysis and gene tree construction. These analyses suggest that some UHB-flanked genes might actually represent divergent forms of other previously described genes. Analysis of the restriction fragment length polymorphism patterns of the UHB-flanked genes among B. burgdorferi isolates demonstrated that these patterns are highly variable among isolates, suggesting that these genes are not phylogenetically conserved. The variable restriction fragment length polymorphism patterns could indicate recombinational activity in these sequences. The presence of numerous copies of the UHB elements and the high degree of homology among UHB-flanked genes could provide the necessary elements to allow for homologous recombination, leading to the generation of recombination variants of UHB gene family members.

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“…Sequence analysis of the cloned 16S rRNA genes revealed 1,536 bases of nearly complete sequences including both primer regions (Head and End). For the phylogenetic analysis, 16S rRNA gene sequences of five Korean isolates were aligned with other published sequences (20)(21)(22)32) and 1,126-base masked sequences were generated. Using these masked sequences, the percent sequence similarity values (Table 2) and evolutionary distance were calculated.…”

Section: Methodsmentioning

confidence: 99%

Genetic Analysis of Borrelia burgdorferi Sensu Lato in Korea Using Genomic Hybridization and 16S rRNA Gene Sequence Determination

Kee

Yoon²,

et al. 1996

Microbiology and Immunology

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Nine Borrelia burgdorferi sensu lato isolated in Korea were subjected to genomic hybridization using 16S rRNA gene probe and specific restriction patterns (HindIII and EcoRV) led these nine Borrelia into five subtypes. The evolutionary relationships of the five isolates corresponding to five RFLP groups were measured through the sequence determination of 16S rRNA gene and phylogenetic analysis. The isolates 935T (group I), 934U and 17Y (Group IIa, IIb) were well clustered with B. garinii and B. afzelii. 5MT and 9MT strains (Group IIIa and Group IIIb) formed a common branch shared with B. afzelii cluster although the evolutionary distance was rather long. So, most of B. burgdorferi sensu lato in Korea was B. afzelii or B. afzelii-related group and some minor group such as B. garinii also existed.

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Identification of a third genomic group of Borrelia burgdorferi through signature nucleotide analysis and 16S rRNA sequence determination

Cited by 65 publications

References 19 publications

Linear chromosomes of Lyme disease agent spirochetes: genetic diversity and conservation of gene order

Linear chromosomes of Lyme disease agent spirochetes: genetic diversity and conservation of gene order

Molecular and evolutionary analyses of a variable series of genes in Borrelia burgdorferi that are related to ospE and ospF, constitute a gene family, and share a common upstream homology box

Genetic Analysis of Borrelia burgdorferi Sensu Lato in Korea Using Genomic Hybridization and 16S rRNA Gene Sequence Determination

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