Identification of a third feline <i><scp>D</scp>emodex</i> species through partial sequencing of the 16<scp>S</scp> r<scp>DNA</scp> and frequency of <i><scp>D</scp>emodex</i> species in 74 cats using a <scp>PCR</scp> assay

Ferreira, Diana; Sastre, Natalia; Ravera, Iván; Altet, Laura; Francino, Olga; Bardagí, Mar; Ferrer, L.

doi:10.1111/vde.12206

Cited by 22 publications

(28 citation statements)

References 19 publications

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“…felis according to their host (cat), the 16S rRNA gene clustered Demodex mites in cats with dogs and humans. Ferreira et al [5] and Frank et al [14] found similar results using 16S rDNA. There are no previously published results for cats using the 18S rRNA gene.…”

Section: Discussionsupporting

confidence: 56%

“…D . canis also have been found in cats [5,15,16]. All these mammals are directly related to human activities (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…Universal 16S primers, described by Ferreira et al [5], were used to amplify an approximately 338-bp fragment of the mitochondrial 16S rRNA gene. Hair samples from wolves, marmots and bats were amplified using real-time qPCR.…”

Section: Methodsmentioning

confidence: 99%

“…Approximately 100 million years ago, the clade Boreoeutheria split into two sister taxa: Euarchontoglires, including primates and rodents, and Laurasiatheria, constituted mainly by hoofed and carnivorous mammals, including cervids, mustelids, canids and felids but also chiroptera [13]. Demodex mites have been described (both morphologically and genetically) in both groups, reflecting the constancy of the hair follicle niche or the high transmissibility of Demodex mites between and within placental mammal species [5,14–20]. We have an extraordinary example of parasitic parallelism in the Demodex genus [6,12,21,22].…”

Section: Introductionmentioning

confidence: 99%

“…The first aim of this investigation was to determine the prevalence of mites (using 16S rDNA primers that successfully amplified clinical Demodex samples [5,18,32,39]) in both captive and free-ranging wild mammals, including gray wolves ( Canis lupus ), yellow-bellied marmots ( Marmota flaviventris ), and four species of vespertillionid bats ( Myotis californicus , M . lucifugus , M .…”

Section: Introductionmentioning

confidence: 99%

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Detection, Prevalence and Phylogenetic Relationships of Demodex spp and further Skin Prostigmata Mites (Acari, Arachnida) in Wild and Domestic Mammals

et al. 2016

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This study was conceived to detect skin mites in social mammals through real-time qPCR, and to estimate taxonomic Demodex and further Prostigmata mite relationships in different host species by comparing sequences from two genes: mitochondrial 16S rRNA and nuclear 18S rRNA. We determined the mite prevalence in the hair follicles of marmots (13%) and bats (17%). The high prevalence found in marmots and bats by sampling only one site on the body may indicate that mites are common inhabitants of their skin. Since we found three different mites (Neuchelacheles sp, Myobia sp and Penthaleus sp) in three bat species (Miotis yumanensis, Miotis californicus and Corynorhinus townsendii) and two different mites (both inferred to be members of the Prostigmata order) in one marmot species (Marmota flaviventris), we tentatively concluded that these skin mites 1) cannot be assigned to the same genus based only on a common host, and 2) seem to evolve according to the specific habitat and/or specific hair and sebaceous gland of the mammalian host. Moreover, two M. yumanensis bats harbored identical Neuchelacheles mites, indicating the possibility of interspecific cross-infection within a colony. However, some skin mites species are less restricted by host species than previously thought. Specifically, Demodex canis seems to be more transmissible across species than other skin mites. D. canis have been found mostly in dogs but also in cats and captive bats. In addition, we report the first case of D. canis infestation in a domestic ferret (Mustela putorius). All these mammalian hosts are related to human activities, and D. canis evolution may be a consequence of this relationship. The monophyletic Demodex clade showing closely related dog and human Demodex sequences also supports this likely hypothesis.

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Section: Discussionsupporting

confidence: 56%

“…D . canis also have been found in cats [5,15,16]. All these mammals are directly related to human activities (e.g.…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%