Identification of a Surface Protein of
            <i>Streptococcus suis</i>
            and Evaluation of Its Immunogenic and Protective Capacity in Pigs

Li, Yuanyi; Martínez, Gabriela; Gottschalk, Marcelo; Lacouture, Sonia; Willson, Philip; Dubreuil, J. Daniel; Jacques, Mario; Harel, Josée

doi:10.1128/iai.74.1.305-312.2006

Cited by 94 publications

(102 citation statements)

References 53 publications

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“…Although the bacterin induced levels of protection similar to those for the conjugate vaccine, this protection was not related to anti-CPS antibodies but probably was related to antiprotein antibodies. Yet, and in contrast to CPS, which is a universal antigen for S. suis type 2, protein antigens vary depending on the strain origin or sequence type (ST) within the same serotype (16,(101)(102)(103)(104)(105). Strains belonging to the same ST (ST1) were used as bacterin and challenge strains, and thus the capacity of such a vaccine preparation to protect against a heterologous challenge (ST25 and ST28 strains) remains to be elucidated (2).…”

Section: Figmentioning

confidence: 99%

Protection against Streptococcus suis Serotype 2 Infection Using a Capsular Polysaccharide Glycoconjugate Vaccine

et al. 2016

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dStreptococcus suis serotype 2 is an encapsulated bacterium and one of the most important bacterial pathogens in the porcine industry. Despite decades of research for an efficient vaccine, none is currently available. Based on the success achieved with other encapsulated pathogens, a glycoconjugate vaccine strategy was selected to elicit opsonizing anti-capsular polysaccharide (anti-CPS) IgG antibodies. In this work, glycoconjugate prototypes were prepared by coupling S. suis type 2 CPS to tetanus toxoid, and the immunological features of the postconjugation preparations were evaluated in vivo. In mice, experiments evaluating three different adjuvants showed that CpG oligodeoxyribonucleotide (ODN) induces very low levels of anti-CPS IgM antibodies, while the emulsifying adjuvants Stimune and TiterMax Gold both induced high levels of IgGs and IgM. Dose-response trials comparing free CPS with the conjugate vaccine showed that free CPS is nonimmunogenic independently of the dose used, while 25 g of the conjugate preparation was optimal in inducing high levels of anti-CPS IgGs postboost. With an opsonophagocytosis assay using murine whole blood, sera from immunized mice showed functional activity. Finally, the conjugate vaccine showed immunogenicity and induced protection in a swine challenge model. When conjugated and administered with emulsifying adjuvants, S. suis type 2 CPS is able to induce potent IgM and isotype-switched IgGs in mice and pigs, yielding functional activity in vitro and protection against a lethal challenge in vivo, all features of a T cell-dependent response. This study represents a proof of concept for the potential of glycoconjugate vaccines in veterinary medicine applications against invasive bacterial infections.

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Section: Figmentioning

confidence: 99%

Protection against Streptococcus suis Serotype 2 Infection Using a Capsular Polysaccharide Glycoconjugate Vaccine

et al. 2016

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“…Rapid detection of all subtypes of S. suis is of great significance for epidemiological survey of S. suis and widely monitoring the prevalence of S. suis in swine. The Sao protein is highly conserved among S. suis strains and Sao-specific antibodies have been shown to react with cell lysates of 28 of 33 S. suis serotypes and 25 of 26 serotype 2 isolates in immunoblots [22] . Sao protein is encoded by three allelic variants of gene of difference lengths, Sao-S (1.5 kb), Sao-M (1.7 kb) and Sao-L (2.0 kb) and Sao-M is the most common type of Sao [24] .…”

Section: Discussionmentioning

confidence: 99%

“…Sao is a common surface protein, containing a C-terminal membrane anchoring LeuPro-X-Thr-Gly (LPXTG) motif and therefore is anchored to the cell wall peptidoglycan by the housekeeping sortase A, which mediates numerous virulence factors during S. suis infection [23] . Sao is highly conserved protein among most S. suis strains and has become an important candidate for the subunit S. suis vaccines [22] . Immunization with recombinant Sao protein was capable of provoking strong humoral antibody responses, diminish clinical signs and bacterial dissemination, improve survival rates and provide cross-serotype protection in pig and mouse vaccination protocols [24] , indicating rSao is a suitable antigen for subunit S. suis vaccine development [25] .…”

Section: Introductionmentioning

confidence: 99%

“…This method has the advantages of low cost, fast application, high sensitivity, ease of use and high reliability, and is possible to detect antibodies or antigens while screening large numbers of samples in a single experiment [7,21] . Surface antigen one (Sao) was identified by screening of a phage display library using convalescent swine sera [22] . Sao is a common surface protein, containing a C-terminal membrane anchoring LeuPro-X-Thr-Gly (LPXTG) motif and therefore is anchored to the cell wall peptidoglycan by the housekeeping sortase A, which mediates numerous virulence factors during S. suis infection [23] .…”

Section: Introductionmentioning

confidence: 99%

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Diagnostik Antijen Olarak Rekombinant Sao-M Protein Kullanılarak Streptococcus suis’in Tanısı Amacıyla PPA-ELİSA Geliştirilmesi

Xia¹,

Zhang²,

Cheng³

et al. 2017

Kafkas Univ Vet Fak Derg

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Streptococcus suis, an important zoonotic agent, is responsible for outbreaks of human infections. The accurate and rapid detection of S. suis may help control the potential outbreak and ameliorate patient outcomes. In the present study, Sao-M was used to establish a horseradish peroxidase enzyme-linked staphylococcal protein A immunosorbent assay (PPA-ELISA) for the diagnosis of S. suis infection. Results of chessboard titration test showed that the optimal concentration of coating antigen and dilution of serum were 8 µg/ml and 1:80, respectively. The cut-off was confirmed as OD450≥0.351 for positive response. The specificity of test indicated that rSao-M had no cross-reaction with antisera against the other 6 species of pathogens. The variation coefficient of intra-batch and inter-batch in the repeating tests was less than 9.5%. Comparative analysis by using conventional ELISA kit and established GDH-based ELISA showed that the present PPA-ELISA has higher specificity and sensitivity than GDH-based ELISA. A total seroprevalence of 6.6% in 500 pig serum samples indicated the method's applicability to detect S. suis infection. Cumulatively, the results suggested that the PPA-ELISA is a rapid, sensitive and specific diagnostic method and could be used as a new tool for large-scale epidemiological surveys and serological diagnosis of S. suis infection. Keywords: Streptococcus suis, PPA-ELISA, Sao-M, Diagnosis Diagnostik Antijen Olarak Rekombinant Sao-M Protein KullanılarakStreptococcus suis'in Tanısı Amacıyla PPA-ELİSA Geliştirilmesi ÖzetStreptococcus suis, insanlarda enfeksiyona neden olan salgınlardan sorumlu önemli bir zoonotik ajandır. S. suis'in doğru ve hızlı bir şekilde tespit edilmesi enfeksiyona bağlı salgınları kontrol edebilir ve hastalığa bağlı etkileri azaltabilir. Bu çalışmada, Sao-M, S suis enfeksiyonunun teşhisi amacıyla peroksidaz enzim-bağlı stafilokokal protein A immün testi (PPA-ELİSA) için kullanılmıştır. Satranç tahtası titrasyon testinin sonuçları, kaplama antijeninin optimal konsantrasyonu ve serum sulandırılmasını sırasıyla 8 µg/mL ve 1:80 olarak göstermiştir. Pozitif cevap için eşik değeri OD450≥0.351 olarak belirlendi. Testin spesifitesi rSao-M'nin diğer 6 patojen türe karşı kullanılan antiserum ile çapraz reaksiyon vermediğini gösterdi. Tekrar testlerinde yığın içi ve yığınlar arası varyasyon katsayısı %9.5'ten az idi. Geleneksel ELİSA kiti ve kurulan GDH tabanlı ELİSA'nın karşılaştırmalı analizinde, mevcut PPA-ELİSA testinin GDH tabanlı ELİSA testinden daha yüksek spesifite ve sensitiviteye sahip olduğunu gösterdi. 500 domuz serumu örneğinde toplam seroprevalansın %6.6 olduğu ve bunun yöntemin S. suis enfeksiyonunun saptanmasında uygunluğunu gösterdi. Sonuçlar, PPA-ELİSA'nın hızlı, duyarlı ve spesifik bir tanı yöntemi olduğunu ve S. suis enfeksiyonunun serolojik tanısı için büyük kapsamlı epidemiyolojik araştırmalarda yeni bir araç olarak kullanılabilirliğini göstermektedir.

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“…They were then separated by 15% SDS-PAGE and transferred onto a polyvinylidene difluoride (PVDF) membrane for the Western blot-based detection of protein 310 as we described previously (16). Here, the first antibody is polyclonal anti-310 protein rabbit serum, which was obtained according to the customary immunization protocol for New Zealand White rabbits (33).…”

Section: Vol 190 2008 Functional Definition and Regulation Of Zur Imentioning

confidence: 99%

Functional Definition and Global Regulation of Zur, a Zinc Uptake Regulator in a Streptococcus suis Serotype 2 Strain Causing Streptococcal Toxic Shock Syndrome

Feng

Zhang

et al. 2008

J Bacteriol

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Zinc is an essential trace element for all living organisms and plays pivotal roles in various cellular processes. However, an excess of zinc is extremely deleterious to cells. Bacteria have evolved complex machineries (such as efflux/influx systems) to control the concentration at levels appropriate for the maintenance of zinc homeostasis in cells and adaptation to the environment. The Zur (zinc uptake regulator) protein is one of these functional members involved in the precise control of zinc homeostasis. Here we identified a zur homologue designated 310 from Streptococcus suis serotype 2, strain 05ZYH33, a highly invasive isolate causing streptococcal toxic shock syndrome. Biochemical analysis revealed that the protein product of gene 310 exists as a dimer form and carries zinc ions. An isogenic gene replacement mutant of gene 310, the ⌬310 mutant, was obtained by homologous recombination. Physiological tests demonstrated that the ⌬310 mutant is specifically sensitive to Zn 2؉ , while functional complementation of the ⌬310 mutant can restore its duration capability, suggesting that 310 is a functional member of the Zur family. Two-dimensional electrophoresis indicated that nine proteins in the ⌬310 mutant are overexpressed in comparison with those in the wild type. DNA microarray analyses suggested that 121 genes in the ⌬310 mutant are affected, of which 72 genes are upregulated and 49 are downregulated. The transcriptome of S. suis serotype 2 with high Zn 2؉ concentrations also showed 117 differentially expressed genes, with 71 upregulated and 46 downregulated. Surprisingly, more than 70% of the genes differentially expressed in the ⌬310 mutant were the same as those in S. suis serotype 2 that were differentially expressed in response to high Zn 2؉ concentration, consistent with the notion that 310 is involved in zinc homeostasis. We thus report for the first time a novel zinc-responsive regulator, Zur, from Streptococcus suis serotype 2.Streptococcus suis 2 is a gram-positive pathogenic bacterium capable of infecting both piglets and humans and causing serious diseases such as arthritis, meningitis, and septicemia (58). It has spread to nearly 20 countries, resulting in more than 400 human cases of severe infection worldwide (38). In particular, two recent outbreaks (in 1998 and 2005) of severe human S. suis serotype 2 infections in China were characterized by streptococcal toxic shock syndrome, implying that highly invasive variants of S. suis serotype 2 might be emerging in Asia (63,72). Virulence factors related to the pathogenesis of S. suis serotype 2 have been partially elucidated and include capsular polysaccharide (56), suilysin (37), muraminidase-released protein (42), and extracellular factor (59). Very recently, our group (8) reported the whole genome sequences of two virulent S. suis serotype 2 isolates (05ZYH33 and 98HAH12).Similar to other transition metals such as iron, manganese,

show abstract

Identification of a Surface Protein of Streptococcus suis and Evaluation of Its Immunogenic and Protective Capacity in Pigs

Cited by 94 publications

References 53 publications

Protection against Streptococcus suis Serotype 2 Infection Using a Capsular Polysaccharide Glycoconjugate Vaccine

Protection against Streptococcus suis Serotype 2 Infection Using a Capsular Polysaccharide Glycoconjugate Vaccine

Diagnostik Antijen Olarak Rekombinant Sao-M Protein Kullanılarak Streptococcus suis’in Tanısı Amacıyla PPA-ELİSA Geliştirilmesi

Functional Definition and Global Regulation of Zur, a Zinc Uptake Regulator in a Streptococcus suis Serotype 2 Strain Causing Streptococcal Toxic Shock Syndrome

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