Identification of a Spindlin homolog in gibel carp (Carassius auratus gibelio)

Wang, Xiao Lei; Sun, Min; Mei, Jie; Gui, Jian‐Fang

doi:10.1016/j.cbpc.2005.02.011

Cited by 10 publications

(14 citation statements)

References 50 publications

(38 reference statements)

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“…Gibel carp, because of the polyploid background and dual reproduction modes of gynogenesis and sexuality, has become a promising fish for screening and isolating reproduction-related genes that are involved in primordial germ cell formation, oocyte maturation and egg fertilization. Using suppression subtractive hybridization and protein purification [51,52], some important genes, such as germ plasm markers vasa [53] and Dazl [54], oocyte maturation-related factors cyclin A2 [55], C-type lectin [52], C1q-like [56][57][58], histone H2A variant h2af1o [59], and maternal-effect factor spindlin [60], have been identified and functionally characterized from gibel carp. For example, Sun et al [61] have observed oocytespecific expression pattern and dynamic distribution of Spindlin during oocyte maturation and egg fertilization, demonstrated its association and interaction with β-tubulin and spindle, and found that its elimination destroys spindle assembly, and thereby disturbs the oocyte-to-embryo transition and first cleavage, which provided the first direct evidence for the critical oocyte-to-embryo transition function of Spindlin in vertebrates.…”

Section: Reproduction Trait and Candidate Reproductionrelated Genesmentioning

confidence: 99%

Molecular basis and genetic improvement of economically important traits in aquaculture animals

2012

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Aquaculture has been believed to be a major Chinese contribution to the world. In recent 20 years, genome and other genetic technologies have promoted significant advances in basic studies on molecular basis and genetic improvement of aquaculture animals, and complete genomes of some main aquaculture animals have been sequenced or announced to be sequenced since the beginning of this century. Here, we review some significant breakthrough progress of aquaculture genetic improvement technologies including genome technologies, somatic cell nuclear transfer and stem cell technologies, outline the molecular basis of several economically important traits including reproduction, sex, growth, disease resistance, cold tolerance and hypoxia tolerance, and present a series of candidate trait-related genes. Finally, some application cases of genetic improvement are introduced in aquaculture animals, especially in China, and several development trends are highlighted in the near future.

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Section: Reproduction Trait and Candidate Reproductionrelated Genesmentioning

confidence: 99%

Molecular basis and genetic improvement of economically important traits in aquaculture animals

2012

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“…In comparison with previous studies in sexual reproduction species, the continuous distribution in the germ plasm may have been initrally discovered in the gynogenetic gibel carp, and appears to have significant implications concerning sex evolution of vertebrates. Significantly, Spindlin, an oocyte to embryo transition factor firstly described in mice [64,65], was also cloned and identified in gibel carp, and its ovary-specific expression and interaction with β-tubulin was observed [66].…”

Section: Gene Identification Involved In the Reproduction And Early Dmentioning

confidence: 99%

Genetic basis and breeding application of clonal diversity and dual reproduction modes in polyploid Carassius auratus gibelio

Gui

Zhou

2010

Sci. China Life Sci.

217

236

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A unisexual species is generally associated with polyploidy, and reproduced by a unisexual reproduction mode, such as gynogenesis, hybridogenesis or parthenogenesis. Compared with other unisexual and polyploid species, gibel carp (Carassius auratus gibelio) has a higher ploidy level of hexaploid. It has undergone several successive rounds of genome polyploidy, and experienced an additional, more recent genome duplication event. More significantly, the dual reproduction modes, including gynogenesis and sexual reproduction, have been demonstrated to coexist in the polyploid gibel carp. This article reviews the genetic basis concerning polyploidy origin, clonal diversity and dual reproduction modes, and outlines the progress in new variety breeding and gene identification involved in the reproduction and early development. The data suggests that gibel carp are under an evolutionary trajectory of diploidization. As a novel evolutionary developmental (Evo-Devo) biology model, this work highlights future perspectives about the functional divergence of duplicated genes and the sexual origin of vertebrate animals.

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“…Identification of oocyte-specific genes was recently initiated using subtractive hybridization in fish because of their diversity and particularities of reproduction modes [20,21]. Oocyte-specific genes, including zp3 [22,23], snx [24], clecdc (c-type lectin domain containing AY157616) [15], c1ql4l (AY662672) [16,25], spin1l (spindling 1-like AY627640) [26], and snrpc [27], were identified and characterized from Carassius auratus gibelio, a unique polyploid fish with dual reproduction modes of gynogenesis and sexual reproduction [28][29][30]. Screening of an oocyte-specific core histone H2A variant in fish was also performed [14].…”

Section: Introductionmentioning

confidence: 99%

Histone H2A Has a Novel Variant in Fish Oocytes1

Yue

Chen

et al. 2009

Biology of Reproduction

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Histone variants and their modification have significant roles in many cellular processes. In this study, we identified and characterized the histone H2A variant h2af1o in fish and revealed its oocyte-specific expression pattern during oogenesis and embryogenesis. Moreover, posttranslational modification of H2af1o was observed that results from phosphorylation during oocyte maturation. To understand the binding dynamics of the novel core histone variant H2af1o in nucleosomes, we cloned ubiquitous gibel carp h2afx as a conventional histone control and investigated the dynamic exchange difference in chromatin by fluorescence recovery after photobleaching. H2af1o has significantly higher mobility in nucleosomes than ubiquitous H2afx. Compared with ubiquitous H2afx, H2af1o has a tightly binding C-terminal and a weakly binding N-terminal. These data indicate that fish oocytes have a novel H2A variant that destabilizes nucleosomes by protruding its N-terminal tail and stabilizes core particles by contracting its C-terminal tail. Our findings suggest that H2af1o may have intrinsic ability to modify chromatin properties during fish oogenesis, oocyte maturation, and early cleavage.

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Identification of a Spindlin homolog in gibel carp (Carassius auratus gibelio)

Cited by 10 publications

References 50 publications

Molecular basis and genetic improvement of economically important traits in aquaculture animals

Molecular basis and genetic improvement of economically important traits in aquaculture animals

Genetic basis and breeding application of clonal diversity and dual reproduction modes in polyploid Carassius auratus gibelio

Histone H2A Has a Novel Variant in Fish Oocytes1

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