Identification of a specific olfactory receptor for 2-isobutyl-3-methoxypyrazine

Pelosi, Paolo; Baldaccini, Natale Emilio; Pisanelli, Anna Maria

doi:10.1042/bj2010245

Cited by 188 publications

(111 citation statements)

References 13 publications

(8 reference statements)

Supporting

Mentioning

107

Contrasting

Unclassified

Order By: Relevance

“…The method described, based on charcoal adsorption of free ligand, was much faster than the Sephadex G-25 microcolumn method [5] previously described. The method, however, was not satisfactory for accurate analysis of binding as 20% differences between replicates were observed.…”

Section: The Binding Assaymentioning

confidence: 99%

“…[4] and a binding protein for 'green' smelling odorants such as 2-isobutyl-3-methoxypyrazine [5]. This latter compound has interesting properties for being a good probe for olfactory binding proteins since it has a very low olfactory threshold in humans ( 2 parts/1012) [6] and specific anosmia can be demonstrated indicating the presence of binding proteins of high affinity to 'green' odorants.…”

mentioning

confidence: 99%

“…This latter compound has interesting properties for being a good probe for olfactory binding proteins since it has a very low olfactory threshold in humans ( 2 parts/1012) [6] and specific anosmia can be demonstrated indicating the presence of binding proteins of high affinity to 'green' odorants. The binding affinity (Kd 1.2 pM) and ligand specificity of this proteins in cows are now well characterised [5,7,IS].…”

mentioning

confidence: 99%

See 2 more Smart Citations

Purification and characterisation of an odorant‐binding protein from cow nasal tissue

Bignetti

Cavaggioni²,

Pelosi

et al. 1985

European Journal of Biochemistry

158

View full text Add to dashboard Cite

Cow nasal tissue contains a protein which shows specific binding activity for ‘green’ smelling compounds such as 2‐isobutyl‐3‐methoxypyrazine. This protein has now been purified using anion‐exchange fast protein liquid chromatography. The protein has a relative molecular mass of 400000–44000, s= 3.1 ± 0.3 S, pI = 4.7 ± 0.1 with an absorbance maximum at 278 nm, and consists of two subunits with an identical relative molecular mass of 19000. It is localised in the soluble fraction of cells from the olfactory mucosa and respiratory mucosa from the middle part of the maxillary and nasal turbinates, and is absent from all other tissues tested.

show abstract

Section: The Binding Assaymentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Purification and characterisation of an odorant‐binding protein from cow nasal tissue

Bignetti

Cavaggioni²,

Pelosi

et al. 1985

European Journal of Biochemistry

158

View full text Add to dashboard Cite

show abstract

“…Although OBPs have been identified as the first protein family involved in the olfactory system since 1980s (Vogt and Riddiford, 1981;Pelosi et al, 1981Pelosi et al, , 1982, scientists could only find few ways to explain the mechanism of olfactory conception during the decade with this isolated protein. This was the situation until the adjacent component, the OR, was identified in mammals (Buck and Axel, 1991).…”

Section: Introductionmentioning

confidence: 99%

Review An overview of odorant-binding protein functions in insect peripheral olfactory reception

Fan¹,

Francis²,

Liu³

et al. 2011

Genet. Mol. Res.

162

129

View full text Add to dashboard Cite

ABSTRACT. Insect olfactory perception involves many aspects of insect life, and can directly or indirectly evoke either individual or group behaviors. Insect olfactory receptors and odorant-binding proteins (OBPs) are considered to be crucial to insect-specific and -sensitive olfaction. Although the mechanisms of interaction between OBPs or OBP/ligand complex with olfactory receptors are still not well understood, it has been shown that many OBPs contribute to insect olfactory perception at various levels. Some of these are numerous and divergent members in OBP family; expression in the olfactory organ at high concentration; a variety of combinational patterns between different OBPs and ligands, but exclusive affinity for one OBP to specific binding ligands; complicated interactions between OBP/ligand complex and transmembrane proteins (olfactory receptors or sensory neuron membrane proteins). First, we review OBPs' ligand-binding property based on OBP structural research and ligandbinding test; then, we review current progress around the points cited above OBP functions in insect peripheral olfactory reception to show the role of such proteins in insect olfactory signal transmission; finally, we discuss applications based on insect OBP research.

show abstract

“…The layer is enriched with odorant binding proteins (OBPs), which belong to the family of lipocalins that help to transport the odor molecules to the olfactory receptors (ORs) [3]. The reception occurs on the olfactory cilia -every olfactory neuron possesses 8-20 [1] of them -and so sensory transmissions can be started.…”

Section: Introductionmentioning

confidence: 99%

Genomics of selected human odorant receptors

et al. 2008

View full text Add to dashboard Cite

Single nucleotide polymorphisms (SNPs) in odorant receptor genes may influence the protein sequence and consequently also the function of the receptors. An analysis of the HapMap data for human OR3A1 was performed and provided evidence that genetic differences subject to ancestry and gender can be recognized. A genomic comparison of individuals shows the diversity of odorant receptor genes and therefore potentially the variety of the sense of smell. At this time, two complete human genomes are available in public domain, which we used for this purpose.

show abstract

Identification of a specific olfactory receptor for 2-isobutyl-3-methoxypyrazine

Cited by 188 publications

References 13 publications

Purification and characterisation of an odorant‐binding protein from cow nasal tissue

Purification and characterisation of an odorant‐binding protein from cow nasal tissue

Review An overview of odorant-binding protein functions in insect peripheral olfactory reception

Genomics of selected human odorant receptors

Contact Info

Product

Resources

About