Identification of a single nucleotide polymorphism of the insulin-like growth factor binding protein 2 gene and its association with growth and body composition traits in the chicken

Abstract

Insulin-like growth factor binding protein 2 regulates a broad spectrum of biological activities involved in growth, development, and differentiation. The current study was designed to investigate the associations of IGFBP2 gene polymorphisms with chicken growth and body composition traits. The Northeast Agricultural University Resource Population (NEAURP) was established by crossing broiler sires, derived from lines at Northeast Agricultural University, that were divergently selected for abdominal fat, with Baier layer dams, a Chinese local breed. The F1 birds were inter-crossed to produce an F2 population. Body weight and body composition traits were measured in the NEAURP. The PCR primers for the intron 2 region of IGFBP2 were designed based on chicken genomic sequence. Nucleotide polymorphisms between parental lines were detected by DNA sequencing. A C/T SNP in intron 2 was detected, and PCR-RFLP methods were then developed to genotype the F2 individuals. The results showed that the IGFBP2 SNP was associated with multiple traits, including BW, metatarsus length, shank length, femur length, shank weight, femur weight, metatarsus claw weight, and abdominal fat weight in the 1,028 NEAURP F2 individuals. This research suggests that IGFBP2 or a tightly linked gene has broad effects on growth and development in the chicken.

Polymorphisms of two neuroendocrinecorrelated genes associated with body weight and reproductive traits in Jinghai yellow chicken

“…The plausible reason for this may be that genetic drift, selection and other factors may affect the allele frequency. These SNPs had significant effect on hatch weight, body weight at 8 weeks of age, egg weight at 300 days and body weight at 300 days which have difference with the aforementioned results (Leng et al, 2009;Li et al, 2006). STAT5b is the important modulator of the growth hormone, growth hormone receptor, IGF, prolactin and insulin signaling pathways.…”

“…In chickens, Leng et al (2009) detected one SNP (C1996A) of the IGFBP-2 gene was associated with abdominal fat weight and percentage of abdominal fat. Li et al (2006) detected a C/T SNP of the IGFBP-2 gene which had effect on BW, metatarsus length, shank length, femur length, shank weight, femur weight, metatarsus claw weight and abdominal fat weight of chicken. DeKoning et al (2003) reported that a QTL for carcass weight was mapped between marker brackets MCW0030 and MCW0236 (about 2.3 to 29 Mb) on GGA7, a region that contains chicken IGFBP2 gene (23 to 24 Mb).…”

Effects of genetic polymorphisms on egg production in indigenous Noi chicken

“…Similarly, IGF-I gene had no effect on egg production of domestic chickens, despite its great contribution in stimulating growth, protein synthesis, cell proliferation and differentiation, egg development (McMurtry et al, 1997;Yun et al, 2005;Li et al, 2006;) and IGF-I/PstI has been proven to have correlation with egg production and days of continuous egglaying (Kim et al, 2004;Li et al, 2009). …”

Insulin-like growth factor I gene polymorphism associated with growth and carcass traits in Thai synthetic chickens

“…These traits are under complex genetic controls; and molecular markers for growth may contribute to more efficient selection for these traits (Li et al, 2006). Marker-assisted selection may increase selection efficiency and improve production performance.…”