Identification of a Single Melanin‐Concentrating Hormone Messenger Ribonucleic Acid in Coho Salmon: Structural Relatedness with 7SL Ribonucleic Acid

Nahon, Jean‐Louis; Presset, Francoise; Schoepfer, Ralf; Vale, Wylie

doi:10.1111/j.1365-2826.1991.tb00260.x

Cited by 24 publications

(12 citation statements)

References 42 publications

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“…We suggest the genes should be named pmcha and pmchb , and pmchla and pmchlb , respectively. pmchla and pmchlb are present in O. keta (named MCH1 and MCH2) (Ono et al, ) , O. mykiss (MCH1 and MCH2) (Baker, Levy, Hall, & Lightman, ), O. tshawytscha (MCH1 and MCH2) (Minth, Qiu, Akil, Watson, & Dixon, ) and O. kisutch (MCH1) (Nahon, Presset, Schoepfer, & Vale, ). We find four variants in the Arctic char ( Salvelinus alpinus) and the Atlantic salmon ( Salmo salar ), which express the mRNAs (Leong et al, ) (Figure and Table ).…”

Section: Resultsmentioning

confidence: 99%

Plasticity for colour adaptation in vertebrates explained by the evolution of the genes pomc, pmch and pmchl

Bertolesi

Zhang

McFarlane

2019

Pigment Cell Melanoma Res

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Different camouflages work best with some background matching colour. Our understanding of the evolution of skin colour is based mainly on the genetics of pigmentation (“background matching”), with little known about the evolution of the neuroendocrine systems that facilitate “background adaptation” through colour phenotypic plasticity. To address the latter, we studied the evolution in vertebrates of three genes, pomc, pmch and pmchl, that code for α‐MSH and two melanin‐concentrating hormones (MCH and MCHL). These hormones induce either dispersion/aggregation or the synthesis of pigments. We find that α‐MSH is highly conserved during evolution, as is its role in dispersing/synthesizing pigments. Also conserved is the three‐exon pmch gene that encodes MCH, which participates in feeding behaviours. In contrast, pmchl (known previously as pmch), is a teleost‐specific intron‐less gene. Our data indicate that in zebrafish, pmchl‐expressing neurons extend axons to the pituitary, supportive of an MCHL hormonal role, whereas zebrafish and Xenopus pmch+ neurons send axons dorsally in the brain. The evolution of these genes and acquisition of hormonal status for MCHL explain different mechanisms used by vertebrates to background‐adapt.

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Section: Resultsmentioning

confidence: 99%

Plasticity for colour adaptation in vertebrates explained by the evolution of the genes pomc, pmch and pmchl

Bertolesi

Zhang

McFarlane

2019

Pigment Cell Melanoma Res

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“…1 ). The oligonucleotides were labeled by tailing with a[ 35S]dCTP or a [,3P]dCTP and terminal deoxynucléotidyl transferase poly merase, as described elsewhere [6],…”

Section: Dma and Rna Probesmentioning

confidence: 99%

Pro-Melanin Concentrating Hormone Messenger Ribonucleic Acid and Peptides Expression in Peripheral Tissues of the Rat

Hervieu

Nahon

1995

Neuroendocrinology

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Melanin-concentrating hormone (MCH) is a cyclic peptide which is predominantly synthetized in the hypothalamus of fish and mammalian brains. In the present paper we examined the expression of MCH mRNA and pro-MCH-derived peptides, i.e. MCH and neuropeptide-(N)-glutamic acid (E) isoleucine (I) amide (NEI), in peripheral tissues of adult rodents. By means of polymerase chain reaction (PCR) of reverse-transcribed RNA, low levels of MCH gene transcripts were detected reliably in testis, stomach, and intestine of Sprague-Dawley and Wistar rats, whereas strong expression was found in hypothalamus. Subsequent sequence analysis of the PCR products verified the authenticity of MCH mRNA found in hypothalamus and stomach. The length of MCH RNA species was measured by Northern blot and multiple MCH RNA species were detected in both rat species. Shortest polyadenylated tails were found in MCH RNAs isolated from the peripheral organs by comparison with hypothalamus MCH RNAs of Wistar rats. In order to localize MCH expression in gastrointestinal and genital tracts of Wistar rats we performed in situ hybridization with specific ³³P-labeled oligo-probes joined to immunocytochemical studies with rat MCH or NEI antisera. In testis, the MCH transcripts and pro-MCH-derived peptide immunoreactivities were found at the periphery of the seminiferous tubules, suggesting expression in Sertoli cells. Studies with MCH oligoprobes and antisera directed towards MCH, NEI and αA-inhibin revealed similar pattern of expression in isolated Sertoli cells from Swiss mice, indicating that MCH RNA species were actually synthesized and translated in these cells. In the gastrointestinal (GI) tract, the cells expressing MCH RNA species and pro-MCH-derived peptides were predominantly expressed in the antral portion of the stomach and duodenum. Strikingly, distinct oligoprobes, recognizing antisense MCH transcript, revealed a pattern of hybridization in the GI tract similar to this observed with oligoprobes revealing the mature MCH mRNA. Furthermore, total RNA from the pyloric junction, duodenum, jejunum, ileum and hypothalamus as well appeared to contain RNA complementary to MCH mRNA suggesting therefore that antisense MCH RNA species may play a general role in regulation of MCH synthesis. Taken together, our present and previous data indicate that authentic MCH RNA species and translational products are expressed in various rodent tissues at the periphery. The cellular location suggests that MCH and associated peptides may play a role in spermatogenesis and in digestive processes.

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“…The elaborating neurons, the transport pathways, and the area of concentration and release (posterior lobe of the pituitary) of MCH were determined by immunocytochemistry by using antibodies to synthetic MCH (Naito et al, 1985;Batten and Baker, 1988). The structure of MCH precursors has been elucidated by cDNA cloning in salmonids (Ono et al, 1988;Minth et al, 1989;Nahon et al, 1991;Baker et al, 1995). The primary structure of salmon MCH (sMCH) is highly conserved in teleosts (Kawauchi et al, 1983;Kawauchi, 1989;Gröneveld et al, 1993).…”

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confidence: 99%

Melanin-concentrating hormone-producing neurons in birds

Cardot¹,

Griffond²,

Risold³

et al. 1999

J. Comp. Neurol.

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The peptidergic melanin‐concentrating hormone (MCH) system was investigated by immunocytochemistry in several birds. MCH perikarya were found in the periventricular hypothalamic nucleus near the paraventricular organ and in the lateral hypothalamic areas. Immunoreactive fibers were very abundant in the ventral pallidum, in the nucleus of the stria terminalis, and in the septum/diagonal band complex, where immunoreactive pericellular nets were prominent. Many fibers innervated the whole preoptic area, the lateral hypothalamic area, and the infundibular region. Some fibers also reached the dorsal thalamus and the epithalamus. The median eminence contained only sparse projections, and the posterior pituitary was not labeled. Thus, in birds, a neurohormonal role for MCH is not likely. Immunoreactive fibers were observed in other regions, such as the intercollicular nucleus, stratum griseum periventriculare (mesencephalic tectum), central gray, nigral complex (especially the ventral tegmental area), reticular areas, and raphe nuclei. Although no physiological investigation concerning the role of MCH has been performed in birds, the distribution patterns of the immunoreactive perikarya and fibers observed suggest that MCH may be involved in functions similar to those described in rats. In particular, the projections to parts of the limbic system (ventropallidal ganglia, septal complex, hypothalamus, dorsal thalamus, and epithalamus) and to structures concerned with visceral and other sensory information integration suggest that MCH acts as a neuromodulator involved in a wide variety of physiological and behavioral adaptations (arousal) with regard to feeding, drinking, and reproduction. J. Comp. Neurol. 411:239–256, 1999. © 1999 Wiley‐Liss, Inc.

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Identification of a Single Melanin‐Concentrating Hormone Messenger Ribonucleic Acid in Coho Salmon: Structural Relatedness with 7SL Ribonucleic Acid

Cited by 24 publications

References 42 publications

Plasticity for colour adaptation in vertebrates explained by the evolution of the genes pomc, pmch and pmchl

Plasticity for colour adaptation in vertebrates explained by the evolution of the genes pomc, pmch and pmchl

Pro-Melanin Concentrating Hormone Messenger Ribonucleic Acid and Peptides Expression in Peripheral Tissues of the Rat

Melanin-concentrating hormone-producing neurons in birds

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