Identification of a selenium-dependent glutathione peroxidase in the blood-sucking insect Rhodnius prolixus

Dias, Felipe A.; Gandara, Ana Caroline P.; Perdomo, Hugo D.; Gonçalves, Renata S.; Oliveira, Cristina da Costa; Oliveira, Raquel L.L.; Citelli, Marta; Polycarpo, Carla; Santesmasses, Didac; Mariotti, Marco; Guigó, Roderic; Braz, Glória R.C.; Missirlis, Fanis; Oliveira, Paulo Roberto

doi:10.1016/j.ibmb.2015.08.007

Cited by 16 publications

(10 citation statements)

References 44 publications

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“…It was previously hypothesized that Sec was replaced by cysteine in an ancient common ancestor of all insects. The discovery of a Sec-based GPx in an insect brings new insight into the evolution of this gene family, indicating that the Sec-GPx genes are more widespread and conserved than previously thought (20). We also observed a recent Sec-to-cysteine conversion in one (RPRC014349) of the two R. prolixus thioredoxin reductases (Fig.…”

Section: Significancementioning

confidence: 51%

Genome of Rhodnius prolixus , an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection

Mesquita

Vionette-Amaral

Lowenberger

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Rhodnius prolixus not only has served as a model organism for the study of insect physiology, but also is a major vector of Chagas disease, an illness that affects approximately seven million people worldwide. We sequenced the genome of R. prolixus, generated assembled sequences covering 95% of the genome (∼702 Mb), including 15,456 putative protein-coding genes, and completed comprehensive genomic analyses of this obligate blood-feeding insect. Although immune-deficiency (IMD)-mediated immune responses were observed, R. prolixus putatively lacks key components of the IMD pathway, suggesting a reorganization of the canonical immune signaling network. Although both Toll and IMD effectors controlled intestinal microbiota, neither affected Trypanosoma cruzi, the causal agent of Chagas disease, implying the existence of evasion or tolerance mechanisms. R. prolixus has experienced an extensive loss of selenoprotein genes, with its repertoire reduced to only two proteins, one of which is a selenocysteine-based glutathione peroxidase, the first found in insects. The genome contained actively transcribed, horizontally transferred genes from Wolbachia sp., which showed evidence of codon use evolution toward the insect use pattern. Comparative protein analyses revealed many lineage-specific expansions and putative gene absences in R. prolixus, including tandem expansions of genes related to chemoreception, feeding, and digestion that possibly contributed to the evolution of a blood-feeding lifestyle. The genome assembly and these associated analyses provide critical information on the physiology and evolution of this important vector species and should be instrumental for the development of innovative disease control methods.

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Section: Significancementioning

confidence: 51%

Genome of Rhodnius prolixus , an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection

Mesquita

Vionette-Amaral

Lowenberger

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

322

418

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“…In fact, the control reaction without the tissue extract confirmed a GPX activity proportional to the extract concentration. A recent study (Dias et al, 2016) on the GPX activity and the gene encoding the selenium-dependent isoform in the kissing bug, Rhodnius prolixus Stål, 1859, supports our results.…”

Section: Antioxidant Capacitysupporting

confidence: 90%

Are antioxidant capacity and oxidative damage related to biological and autecological characteristics in aquatic insects?

et al. 2016

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“…Although the specific functions have not been well studied, arthropods are known to express Sec-containing GPx proteins, most of which diverge somewhat from the mammalian subfamilies [79]. A recent phylogenetic analysis suggests that arthropod GPx homologues characterised to date form a clade with mammalian GPx3 [82], an extracellular form with broad substrate specificity [83]. …”

Section: Resultsmentioning

confidence: 99%

De novo assembly and analysis of changes in the protein-coding transcriptome of the freshwater shrimp Paratya australiensis (Decapoda: Atyidae) in response to acid sulfate drainage water

2016

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BackgroundThe atyid shrimp Paratya australiensis occurs in surface freshwater habitats throughout eastern Australia and has been used to study the ecotoxicology of contaminants such as pesticides and metals. The acidification of surface water that can occur after acid sulfate material in soils and sediments is oxidised and subsequently re-wetted is a serious environmental issue in coastal regions and inland riverine floodplains worldwide. Solubilisation of soil-associated minerals can result in high waterborne concentrations of mineral salts and dissolved metals, which together with low pH represent a potential threat to aquatic ecosystems in affected regions. The aims of the present study were to gain insight into stress responses induced by exposure to acid drainage water (ADW) in P. australiensis by determining changes in the abundance of protein-coding transcripts and to generate a comprehensive transcriptomic resource to facilitate further research into gene regulation or protein structure and function in this species. Adult P. australiensis were exposed for 24 h to undiluted ADW, 50 % ADW diluted in river water, or to river water as control, and high-throughput mRNA sequencing (RNA-Seq) conducted on whole-body tissues. A reference transcriptome was generated using de novo assembly and putative protein-coding regions were identified and annotated. Changes in transcript abundance in response to ADW exposure were determined by aligning reads to the reference transcriptome and quantifying coverage.ResultsA high proportion of arthropod benchmarking universal single-copy orthologues were present in the reference transcriptome. Functions associated with cuticle biosynthesis and oxidative stress were significantly enriched in the lists of transcripts exhibiting differential abundance in either direction after exposure to 50 % or 100 % ADW. Transcripts involved in osmoregulation exhibited decreased abundance following exposure to ADW. The transcriptome contained full-length coding sequences for numerous proteins known to be involved in environmental response pathways, including two putative metallothioneins, four glutathione peroxidases and 19 nuclear receptors.ConclusionsThe results of the present study provide insight into stress response pathways induced in crustaceans by short-term exposure to multiple stressors present in ADW such as low pH, high salinity and dissolved metals, and represent a resource for future toxicogenomics and protein functional studies in P. australiensis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3208-y) contains supplementary material, which is available to authorized users.

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Identification of a selenium-dependent glutathione peroxidase in the blood-sucking insect Rhodnius prolixus

Cited by 16 publications

References 44 publications

Genome of Rhodnius prolixus , an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection

Genome of Rhodnius prolixus , an insect vector of Chagas disease, reveals unique adaptations to hematophagy and parasite infection

Are antioxidant capacity and oxidative damage related to biological and autecological characteristics in aquatic insects?

De novo assembly and analysis of changes in the protein-coding transcriptome of the freshwater shrimp Paratya australiensis (Decapoda: Atyidae) in response to acid sulfate drainage water

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