Identification of a Rice stripe necrosis virus resistance locus and yield component QTLs using Oryza sativa × O. glaberrima introgression lines

Abstract

BackgroundDeveloping new population types based on interspecific introgressions has been suggested by several authors to facilitate the discovery of novel allelic sources for traits of agronomic importance. Chromosome segment substitution lines from interspecific crosses represent a powerful and useful genetic resource for QTL detection and breeding programs.ResultsWe built a set of 64 chromosome segment substitution lines carrying contiguous chromosomal segments of African rice Oryza glaberrima MG12 (acc. IRGC103544) in the genetic background of Oryza sativa ssp. tropical japonica (cv. Caiapó). Well-distributed simple-sequence repeats markers were used to characterize the introgression events. Average size of the substituted chromosomal segments in the substitution lines was about 10 cM and covered the whole donor genome, except for small regions on chromosome 2 and 4. Proportions of recurrent and donor genome in the substitution lines were 87.59% and 7.64%, respectively. The remaining 4.78% corresponded to heterozygotes and missing data. Strong segregation distortion was found on chromosomes 3 and 6, indicating the presence of interspecific sterility genes. To illustrate the advantages and the power of quantitative trait loci (QTL) detection using substitution lines, a QTL detection was performed for scored traits. Transgressive segregation was observed for several traits measured in the population. Fourteen QTLs for plant height, tiller number per plant, panicle length, sterility percentage, 1000-grain weight and grain yield were located on chromosomes 1, 3, 4, 6 and 9. Furthermore, a highly significant QTL controlling resistance to the Rice stripe necrosis virus was located between SSR markers RM202-RM26406 (44.5-44.8 cM) on chromosome 11.ConclusionsDevelopment and phenotyping of CSSL libraries with entire genome coverage represents a useful strategy for QTL discovery. Mapping of the RSNV locus represents the first identification of a genetic factor underlying resistance to this virus. This population is a powerful breeding tool. It also helps in overcoming hybrid sterility barriers between species of rice.

Yield Potential and Physiological and Morphological Characteristics Related to Yield Performance inOryza glaberrimaSteud.

“…glaberrima (Brar, 2005). In collaboration with Institut de Recherche pour le Développement (IRD), France, Centro International de Agricultura Tropical (CIAT) based in Columbia has developed chromosome segment substitution lines (CSSLs) with O. glaberrima introgressions into O. sativa (Tohme, 2008), and a rice stripe necrosis virus resistance locus was identified using CSSLs from Caiapo (O. sativa, subgroup japonica)×MG 12 (O. glaberrima) (Gutiérrez et al, 2010). The CSSLs developed by CIAT and IRD have been used by collaborators for the localization of genes/QTLs for important traits such as drought resistance, panicle architecture, root development, iron toxicity resistance, bacterial leaf blight resistance and other agronomic traits (Tohme, 2008).…”

Impact of Genomic Technologies on Chickpea Breeding Strategies

“…The whole donor genome is represented by a set of small, contiguous overlapping fragments. Such introgression lines (ILs) have been utilized in rice [112][113][114], tomato [115,116], soybean [117] and groundnut [118].…”

Diversifying Sunflower Germplasm by Integration and Mapping of a Novel Male Fertility Restoration Gene

“…In plants, segregation distortion has been encountered in maize (Mangelsdor and Jones 1926), rice (McCouch et al 1988), wheat (Zhang and Dvorák 1990;Faris et al 1998;Kumar et al 2007), barley (Graner et al 1991;Li et al 2010), tobacco (Cameron and Moav 1957), tomato (Paterson et al 1988), alfalfa (Echt et al 1994), and coffee (Ky et al 2000). Segregation distortion, also called "meiotic drive," may be caused by genetic elements, including gametic selection (pollen tube competition, lethal pollen, and preferential fertilization), zygotic selection, interspecific sterility genes (S), and chromosome translocation (Lyttle 1991;Kumar et al 2007;Gutiérrez et al 2010;Liu et al 2010). It has been suggested that meiotic drive elements are highly important for the evolution of recombination and sexual reproduction (Hurst and Werren 2001;Jaenike 2001;Li et al 2010).…”

“…Moreover, only one amphiploid was discovered to contain the Rf gene for CMS 514A after testing 59 different germplasm sources, including the maintainer of CMS 514A, which restores fertility to other CMS types (Wang et al 2007; this study). Taken together that CMS 514A has an H. tuberosus cytoplasm and the Rf 6 gene was from the wild species H. angustifolius, the segregation distortion may be caused by several factors, such as gametic selection, interspecific S gene, and chromosome translocation (Lyttle 1991;Kumar et al 2007;Gutiérrez et al 2010;Liu et al 2010).…”