Identification of a quinazoline alkaloid produced by<i>Penicillium vinaceum</i>, an endophytic fungus from<i>Crocus sativus</i>

Zheng, Cheng‐Jian; Li, Lin; Zou, Jing-ping; Han, Ting; Qin, Lu‐Ping

doi:10.3109/13880209.2011.569726

Cited by 33 publications

(16 citation statements)

References 14 publications

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“…22 In a third study conducted in China only Penicillium vinaceum was isolated from corms of C. sativus growing in the Changxing Island. 12 In our study the dominant endophytic fungi associated with saffron roots belonged to different genera and species than those reported in the previous studies. The striking differences in community composition Values with different letters differ significantly (P < 0.05)…”

Section: Discussioncontrasting

confidence: 48%

“…At the international level studies focused on endophytic fungi recovered from corms and stems of saffron, but to our knowledge no study has looked at the roots that are the entry point for endophytic fungi. [12][13] In this context our main objective was the isolation of endophytic fungi from roots of saffron growing in Morocco, their molecular identification and assessment of their possible antibacterial and antioxidant activities through the extraction of secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

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Endophytic Fungi Isolated from Crocus sativus L. (Saffron) as a Source of Bioactive Secondary Metabolites

Chamkhi¹,

Sbabou²,

Aurag³

2018

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Introduction: Endophytic fungi are becoming an important source of new natural bioactive products. Many interesting endophytic fungi have been isolated from traditional medicinal plants. Crocus sativus L. (saffron) is one of the most expensive and rarest spices in the world, used as a dye, aroma and for medicinal purposes. This study reports on the molecular characterization of endophytic fungi isolated from roots of saffron plants growing in Taliouine-Morocco, and the examination of the antibacterial and antioxidant activities of secondary metabolites extracted from these endophytes. Methods and Material: The fungi were isolated from sterilized saffron root fragments and identified using internal transcribed spacer (ITS) rRNA gene sequence analysis. Three endophytes were fermented and extracted with ethyl acetate (EtOAc) and chloroform (CHCl 3). The antibacterial activity of fungi extracts was evaluated using the Agar Diffusion Method against six strains, Bacillus sp., Stenotrophomonas sp., Pseudomonas putida, Pantoea sp., Luteibacter sp. and Escherichia coli. The antioxidant activity was tested by using the α,α-diphenyl-β-picrylhydrazyl (DPPH) analysis and β-Carotene bleaching test (BCBT) methods. Results: Sixty fungal isolates were recovered and purified from saffron roots. ITS rDNA sequences were 99-100% identical to three different species: Rhizopus oryzae, Aspergillus fumigatiaffinis and Aspergillus niger. Both Rhizopus oryzae extracts had high antibacterial activity against most of the bacteria tested, while Aspergillus niger and Rhizopus oryzae extracts showed an antioxidant capacity using DPPH and BCBT methods, respectively. Conclusion: The fungal endophytes inhabiting saffron roots could be a potential source of natural plant bioactive secondary metabolites especially for antibacterial and antioxidant purposes.

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Section: Discussioncontrasting

confidence: 48%

Section: Introductionmentioning

confidence: 99%

Endophytic Fungi Isolated from Crocus sativus L. (Saffron) as a Source of Bioactive Secondary Metabolites

Chamkhi¹,

Sbabou²,

Aurag³

2018

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“…Plant associated fungal interactions based on cultivation independent metagenomic approach are under-represented in literature and the routine cultivation dependent approaches to study plant -fungal associations are dominated by endophytic and arbuscular mycorrhizal fungi [ 6 , 12 – 19 ]. Previously studies on saffron–fungal associations have been done mostly on fungal endophytes and the bioactive compounds of these endophytes [ 15 – 17 ]. Since only <5% of the fungi can be retrieved by routine laboratory cultivation dependent techniques [ 20 ], the complete fungal diversity can only be assessed by the cultivation-independent metagenomic approaches [ 10 , 21 – 29 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Metagenomics Reveal Phylum Level Temporal and Spatial Changes in Mycobiome of Belowground Parts of Crocus sativus

et al. 2016

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Plant-fungal associations have been explored by routine cultivation based approaches and cultivation based approaches cannot catalogue more than 5% of fungal diversity associated with any niche. In the present study, an attempt has been made to catalogue fungal diversity associated with belowground parts i.e. rhizosphere and cormosphere, of Crocus sativus (an economically important herb) during two growth stages, using cultivation independent ITS gene targeted approach, taking bulk soil as reference. The 454 pyrosequencing sequence data analysis suggests that the fungal diversity was niche and growth stage specific. Fungi diversity, in the present case, was not only different between the two organs (roots and corm) but the dominance pattern varies between the cormosphere during two growth stages. Zygomycota was dominant fungal phylum in the rhizosphere whereas Basidiomycota was dominant in cormosphere during flowering stage. However in cormosphere though Basidiomycota was dominant phylum during flowering stage but Zygomycota was dominant during dormant stage. Interestingly, in cormosphere, the phyla which was dominant at dormant stage was rare at flowering stage and vice-versa (Basidiomycota: Flowering = 93.2% Dormant = 0.05% and Zygomycota: Flowering = 0.8% Dormant = 99.7%). At genus level, Rhizopus was dominant in dormant stage but was rare in flowering stage (Rhizopus: Dormant = 99.7% Flowering = 0.55%). This dynamics is not followed by the bulk soil fungi which was dominated by Ascomycota during both stages under study. The genus Fusarium, whose species F. oxysporum causes corm rot in C. sativus, was present during both stages with slightly higher abundance in roots. Interestingly, the abundance of Rhizopus varied a great deal in two stages in cormosphere but the abundance of Fusarium was comparable in two growth stages (Bulk soil Flowering = 0.05%, Rhizosphere Flowering = 1.4%, Cormosphere Flowering = 0.06%, Bulk soil Dormant = 2.47% and cormosphere dormant = 0.05%). This is the first report on the fungal diversity associated with the root of Crocus sativus and first report on the fungi associated with corm of any plant with the temporal and spatial variation in the fungal community structure.

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“…Recent reviews report the characterization of 138 secondary metabolites from endophytic fungi before 2000 [18] with an additional 184 reported by 2006 [19]. These metabolites encompass a diverse range of structures including alkaloids [20], terpenoids [21], quinones [22], and peptides, xanthones and phenols [23]. Bioactivity has been observed against cancer cell lines [24] [25], pathogenic bacteria [26] [27] and fungi (e.g., [28]), and against eukaryotic parasites such as the causal agents of malaria, leishmaniasis and Chagas' disease (e.g., [29] [30]).…”

Section: Introductionmentioning

confidence: 99%

Bioactivity of Fungal Endophytes as a Function of Endophyte Taxonomy and the Taxonomy and Distribution of Their Host Plants

et al. 2013

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Fungal endophytes – fungi that grow within plant tissues without causing immediate signs of disease – are abundant and diverse producers of bioactive secondary metabolites. Endophytes associated with leaves of tropical plants are an especially exciting and relatively untapped source of novel compounds. However, one major challenge in drug discovery lies in developing strategies to efficiently recover highly bioactive strains. As part of a 15-year drug discovery project, foliar endophytes were isolated from 3198 plant samples (51 orders, 105 families and at least 232 genera of angiosperms and ferns) collected in nine geographically distinct regions of Panama. Extracts from culture supernatants of >2700 isolates were tested for bioactivity (in vitro percent inhibition of growth, % IG) against a human breast cancer cell line (MCF-7) and the causative agents of malaria, leishmaniasis, and Chagas' disease. Overall, 32.7% of endophyte isolates were highly active in at least one bioassay, including representatives of diverse fungal lineages, host lineages, and collection sites. Up to 17% of isolates tested per assay were highly active. Most bioactive strains were active in only one assay. Fungal lineages differed in the incidence and degree of bioactivity, as did fungi from particular plant taxa, and greater bioactivity was observed in endophytes isolated from plants in cloud forests vs. lowland forests. Our results suggest that using host taxonomy and forest type to tailor plant collections, and selecting endophytes from specific orders or families for cultivation, will markedly increase the efficiency and efficacy of discovering bioactive metabolites for particular pharmaceutical targets.

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Identification of a quinazoline alkaloid produced byPenicillium vinaceum, an endophytic fungus fromCrocus sativus

Cited by 33 publications

References 14 publications

Endophytic Fungi Isolated from Crocus sativus L. (Saffron) as a Source of Bioactive Secondary Metabolites

Endophytic Fungi Isolated from Crocus sativus L. (Saffron) as a Source of Bioactive Secondary Metabolites

Comparative Metagenomics Reveal Phylum Level Temporal and Spatial Changes in Mycobiome of Belowground Parts of Crocus sativus

Bioactivity of Fungal Endophytes as a Function of Endophyte Taxonomy and the Taxonomy and Distribution of Their Host Plants

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