Identification of a putative kinase interacting domain in the durum wheat catalase 1 (TdCAT1) protein

Ghorbel, Mohamed Habib; Haddaji, Najla; Feki, Kaouthar; Tounsi, Sana; Chihaoui, Mejda; Alghamdi, Ahmad; Mseddi, Khalil; Brini, Faïçal

doi:10.1016/j.heliyon.2023.e18916

Cited by 3 publications

(3 citation statements)

References 63 publications

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“…Interestingly, the catalase activity of TdCAT1 increased gradually with increasing concentrations of cations in the medium, with the most important effect being enregistered in the presence of Fe 2+ and Mn 2+ [13]. In addition, those proteins can interact with other proteins, as previously described, such as the CaM/Ca 2+ complex [13] and protein kinases [62]. In addition, AvCAT1/2 and 3 are structural constituents of ribosomes, whereas AvCAT1/2 /3 and 5 have 5S rRNA binding functions (Figure 6).…”

Section: Discussionmentioning

confidence: 51%

“…In fact, treatment of TdCAT1 by phosphatase inhibited the catalytic activity of the protein [14]. Interestingly, it has been recently shown that TdCAT1 proteins could be phosphorylated in vitro in the presence of wheat Mitogen-Activated Protein Kinase protein (TMPK3) and that the presence of TMPK3 enhanced the catalytic activity of the catalase [62]. In the current work, in silico analyses showed that AvCAT proteins could be phosphorylated by MAPKs.…”

Section: Discussionmentioning

confidence: 65%

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Genome-Wide Investigation and Expression Analysis of the Catalase Gene Family in Oat Plants (Avena sativa L.)

Ghorbel,

Zribi,

Chihaoui

et al. 2023

Plants

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Through the degradation of reactive oxygen species (ROS), different antioxidant enzymes, such as catalase (CAT), defend organisms against oxidative stress. These enzymes are crucial to numerous biological functions, like plant development and defense against several biotic and abiotic stresses. However, despite the major economic importance of Avena sativa around the globe, little is known about the CAT gene’s structure and organization in this crop. Thus, a genome-wide investigation of the CAT gene family in oat plants has been carried out to characterize the potential roles of those genes under different stressors. Bioinformatic approaches were used in this study to predict the AvCAT gene’s structure, secondary and tertiary protein structures, physicochemical properties, phylogenetic tree, and expression profiling under diverse developmental and biological conditions. A local Saudi oat variety (AlShinen) was used in this work. Here, ten AvCAT genes that belong to three groups (Groups I–III) were identified. All identified CATs harbor the two conserved domains (pfam00199 and pfam06628), a heme-binding domain, and a catalase activity motif. Moreover, identified AvCAT proteins were located in different compartments in the cell, such as the peroxisome, mitochondrion, and cytoplasm. By analyzing their promoters, different cis-elements were identified as being related to plant development, maturation, and response to different environmental stresses. Gene expression analysis revealed that three different AvCAT genes belonging to three different subgroups showed noticeable modifications in response to various stresses, such as mannitol, salt, and ABA. As far as we know, this is the first report describing the genome-wide analysis of the oat catalase gene family, and these data will help further study the roles of catalase genes during stress responses, leading to crop improvement.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 65%

Genome-Wide Investigation and Expression Analysis of the Catalase Gene Family in Oat Plants (Avena sativa L.)

Ghorbel,

Zribi,

Chihaoui

et al. 2023

Plants

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“…A recent study has shown that the catalase activity of durum wheat catalase (TdCAT1) was hindered following protein dephosphorylation through treatment with λ-phosphatase [ 61 ]. In addition, TdCAT1 interacts with mitogen-activated protein kinase 3 (TMPK3) through its N-terminal region and enhances its catalytic activity [ 62 ]. Our analysis in this study revealed that MAPKs can phosphorylate CAT proteins.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of Catalase Gene Families in Triticeae

Ghorbel,

Zribi,

Haddaji

et al. 2023

Plants

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Aerobic metabolism in plants results in the production of hydrogen peroxide (H2O2), a significant and comparatively stable non-radical reactive oxygen species (ROS). H2O2 is a signaling molecule that regulates particular physiological and biological processes (the cell cycle, photosynthesis, plant growth and development, and plant responses to environmental challenges) at low concentrations. Plants may experience oxidative stress and ultimately die from cell death if excess H2O2 builds up. Triticum dicoccoides, Triticum urartu, and Triticum spelta are different ancient wheat species that present different interesting characteristics, and their importance is becoming more and more clear. In fact, due to their interesting nutritive health, flavor, and nutritional values, as well as their resistance to different parasites, the cultivation of these species is increasingly important. Thus, it is important to understand the mechanisms of plant tolerance to different biotic and abiotic stresses by studying different stress-induced gene families such as catalases (CAT), which are important H2O2-metabolizing enzymes found in plants. Here, we identified seven CAT-encoding genes (TdCATs) in Triticum dicoccoides, four genes in Triticum urartu (TuCATs), and eight genes in Triticum spelta (TsCATs). The accuracy of the newly identified wheat CAT gene members in different wheat genomes is confirmed by the gene structures, phylogenetic relationships, protein domains, and subcellular location analyses discussed in this article. In fact, our analysis showed that the identified genes harbor the following two conserved domains: a catalase domain (pfam00199) and a catalase-related domain (pfam06628). Phylogenetic analyses showed that the identified wheat CAT proteins were present in an analogous form in durum wheat and bread wheat. Moreover, the identified CAT proteins were located essentially in the peroxisome, as revealed by in silico analyses. Interestingly, analyses of CAT promoters in those species revealed the presence of different cis elements related to plant development, maturation, and plant responses to different environmental stresses. According to RT-qPCR, Triticum CAT genes showed distinctive expression designs in the studied organs and in response to different treatments (salt, heat, cold, mannitol, and ABA). This study completed a thorough analysis of the CAT genes in Triticeae, which advances our knowledge of CAT genes and establishes a framework for further functional analyses of the wheat gene family.

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Pepper catalase: a broad analysis of its modulation during fruit ripening and by nitric oxide

González-Gordo,

López-Jaramillo,

Rodríguez-Ruiz

et al. 2024

Biochemical Journal

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Catalase is a major antioxidant enzyme located in plant peroxisomes that catalyzes the decomposition of H2O2. Based on our previous transcriptomic (RNA-Seq) and proteomic (iTRAQ) data at different stages of pepper (Capsicum annuum L.) fruit ripening and after exposure to nitric oxide (NO) enriched atmosphere, a broad analysis has allowed us to characterize the functioning of this enzyme. Three genes were identified, and their expression was differentially modulated during ripening and by NO gas treatment. A dissimilar behavior was observed in the protein expression of the encoded protein catalases (CaCat1–CaCat3). Total catalase activity was down-regulated by 50% in ripe (red) fruits concerning immature green fruits. This was corroborated by non-denaturing polyacrylamide gel electrophoresis, where only a single catalase isozyme was identified. In vitro analyses of the recombinant CaCat3 protein exposed to peroxynitrite (ONOO−) confirmed, by immunoblot assay, that catalase underwent a nitration process. Mass spectrometric analysis identified that Tyr348 and Tyr360 were nitrated by ONOO−, occurring near the active center of catalase. The data indicate the complex regulation at gene and protein levels of catalase during the ripening of pepper fruits, with activity significantly down-regulated in ripe fruits. Nitration seems to play a key role in this down-regulation, favoring an increase in H2O2 content during ripening. This pattern can be reversed by the exogenous NO application. While plant catalases are generally reported to be tetrameric, the analysis of the protein structure supports that pepper catalase has a favored quaternary homodimer nature. Taken together, data show that pepper catalase is down-regulated during fruit ripening, becoming a target of tyrosine nitration, which provokes its inhibition.

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Identification of a putative kinase interacting domain in the durum wheat catalase 1 (TdCAT1) protein

Cited by 3 publications

References 63 publications

Genome-Wide Investigation and Expression Analysis of the Catalase Gene Family in Oat Plants (Avena sativa L.)

Genome-Wide Investigation and Expression Analysis of the Catalase Gene Family in Oat Plants (Avena sativa L.)

Genome-Wide Identification and Expression Analysis of Catalase Gene Families in Triticeae

Pepper catalase: a broad analysis of its modulation during fruit ripening and by nitric oxide

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