Identification of a potyvirus associated with mosaic disease of <i>Narcissus </i>sp. in India

Shekhawat, Dr. Krishna Yadav, Dr. Neharika Saxena*, Dr. Surendra Singh; Khan, J. A.

doi:10.1111/j.1365-3059.2007.01655.x

Cited by 10 publications

(9 citation statements)

References 2 publications

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“…1d). Association of such symptoms in N. tazetta was also observed earlier with potyvirus in India (Aminuddin and Raj 1999;Yadav and Khan 2007) and other countries (Wylie et al 2014;Chen et al 2006). For characterization of virus, infected leaf samples of narcissus were collected and stored in − 80 °C till further used.…”

Section: Introductionmentioning

confidence: 64%

“…(Brunt 1995;Wylie and Jones 2012). Amongst all, potyviruses are the most prevalent in N. tazetta (Brunt 1995;Aminuddin and Raj 1999;Yadav and Khan 2007) and infected plants in general exhibit the symptoms of leaf chlorotic stripes, plant stunting, and flower colour breaking. The vegetative mean of propagating bulbs is a particular problem for virus disease dissemination in all bulbous crops including narcissus.…”

Section: Introductionmentioning

confidence: 99%

“…An uncharacterized potyvirus was detected from N. tazetta cv. Paperwhite plants associated with yellow stripes symptoms (Aminuddin and Raj 1999) which was identified as Lycoris potyvirus, based on the partial 3′ sequence (Yadav and Khan 2007). Moreover, occurrence of a previously unidentified Cyrtanthus elatus virus A (CEVA) on N. tazetta cv.…”

Section: Introductionmentioning

confidence: 99%

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Full-length genome sequence of Cyrtanthus elatus virus-A isolated from Narcissus tazetta in India

Raj

Kaur

Agrawal³

et al. 2018

3 Biotech

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L. is a bulbous ornamental plant popular for its notable fragrant flowers which make it the plant of high importance. In spite of its economic value, narcissus is found to be susceptible for a number of diseases borne by fungi, bacteria, nematodes, and viruses. A potyvirus, - isolate NBRI16 (CEVA-NBRI16), associated with leaf chlorotic stripe disease of . cv. Paperwhite was reported for first time in India from our laboratory based on the partial coat protein gene sequence. In present study, the full-length genomic sequence of CEVA-NBRI16 is determined which consists of 9942 nucleotides, excluding the polyA tail, and encodes a single large polyprotein of 3102 amino acids with the genomic features typical of a potyvirus. It shares highest 93% nucleotide sequence identity and closest phylogenetic relationship with sequences of CEVA-Marijiniup7-1 and CEVA-Marijiniup7-2, both reported from Australia on host. The full-length genomic sequence of CEVA from narcissus plant is being reported for the first time from India.

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Section: Introductionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Full-length genome sequence of Cyrtanthus elatus virus-A isolated from Narcissus tazetta in India

Raj

Kaur

Agrawal³

et al. 2018

3 Biotech

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“…Literature survey revealed that infection of said viruses reduced the quality and productivity of narcissus bloom and bulbs (Hanks and Chastagner 2018). Among them, potyviruses are the most prevalent viruses of narcissus including NYSV, NLSYV, NDV, CEVA and OrMV (Chen et al 2006;Yadav and Khan 2008;Kumar et al 2015;Ohshima et al 2016;Raj et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Sequence analyses of RT-PCR products obtained from seven infected leaf samples revealed existence of three potyvirus species in Indian narcissus (Narcissus tazetta L.)

et al. 2020

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Potyvirus species associated with yellow leaf stripe disease of Indian narcissus (Narcissus tazetta L.) var. Paperwhite has been studied by sequence analyses of ~ 1.5 kb genomic fragments obtained from seven RT-PCR amplifications of infected samples. Sequence analysis revealed the occurrence of three potyvirus species: cyrtanthus elatus virus-A (CEVA: KF430815, KF430816, KM066973, KM066974); narcissus yellow stripe virus (NYSV: KM066972, JQ686724) and narcissus degeneration virus (NDV: MK572806). The existence of three potyvirus species: CEVA, NYSV and NDV are being reported in Indian narcissus.

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“…The sequence identity matrix between amino acid sequences and nucleotide sequences was calculated using the BioEdit software version 5.09.04. Amino acid sequences were aligned using Clustal W (Verma et al, 2016;Yadav and Khan, 2015) and Neighbor-joining tree was generated using MEGA v. 7.0 program (Kumar et al, 2016).…”

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confidence: 99%

Genetic Variability of Multifunctional HC-Pro Gene of <I>Lettuce mosaic virus</I> in Northern Region of India

Mishra¹,

Verma²,

Gaur³

2017

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Lettuce mosaic virus (LMV) is an economically important pathogen causing mosaic disease in spinach (Spinacia oleracea). Isolates of LMV originating from different geographical regions in northern India were collected during the survey of 2013-2014. Lettuce mosaic virus (LMV) belongs to Potyviridae family (genus Potyvirus) and is mainly transmitted by aphids. This study covered the genetic variability of Helper component proteinase (HC-Pro) gene of LMV isolates. HC-Pro is multifunctional activity gene, which helps in transmission of virus. The amplified PCR products of ~1.3 kilo basepairs (kb) were obtained from five different isolates, sequenced and deposited in NCBI GenBank. Two isolates R4 and HR1 from Rajasthan (KJ558375, KM501033), isolate R5 from Haryana (KP635365), isolate R6 from Uttar Pradesh (KP635366) and isolate R4 from Delhi (KP635367)]. The phylogenetic analysis of LMV HC-Pro sequences is showed divergence with other isolates. These isolates showed two different clusters visa -vis diversity amongst each other.

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Identification of a potyvirus associated with mosaic disease of Narcissus sp. in India

Cited by 10 publications

References 2 publications

Full-length genome sequence of Cyrtanthus elatus virus-A isolated from Narcissus tazetta in India

Full-length genome sequence of Cyrtanthus elatus virus-A isolated from Narcissus tazetta in India

Sequence analyses of RT-PCR products obtained from seven infected leaf samples revealed existence of three potyvirus species in Indian narcissus (Narcissus tazetta L.)

Genetic Variability of Multifunctional HC-Pro Gene of <I>Lettuce mosaic virus</I> in Northern Region of India

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