Identification of a potential transcriptional regulator encoded by grass carp reovirus

Yu, Fei; Wang, Hao; Wang, Longlong; Li, Wanjuan; Lu, Liqun

doi:10.1007/s00705-019-04204-1

Cited by 7 publications

(4 citation statements)

References 44 publications

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“…ORF 32 encodes a DNA ligase that could participate in the processes of DNA replication, recombination, and repair. ORF35 encodes an HTH-type transcriptional regulator, which is usually found to be a DNA-binding domain in the transcriptional regulator, and associated with regulatory metabolism in gene expression (Yu et al, 2019 ). ORF 47, codifying for an ATP-dependent RecD-like DNA helicase, is implicated in DNA repair (Köppen et al, 1995 ).…”

Section: Resultsmentioning

confidence: 99%

The First Cbk-Like Phage Infecting Erythrobacter, Representing a Novel Siphoviral Genus

Guo

Zou

et al. 2022

Front. Microbiol.

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Erythrobacter is an important and widespread bacterial genus in the ocean. However, our knowledge about their phages is still rare. Here, a novel lytic phage vB_EliS-L02, infecting Erythrobacter litoralis DSM 8509, was isolated and purified from Sanggou Bay seawater, China. Morphological observation revealed that the phage belonged to Cbk-like siphovirus, with a long prolate head and a long tail. The host range test showed that phage vB_EliS-L02 could only infect a few strains of Erythrobacter, demonstrating its potential narrow-host range. The genome size of vB_EliS-L02 was 150,063 bp with a G+C content of 59.43%, encoding 231 putative open reading frames (ORFs), but only 47 were predicted to be functional domains. Fourteen auxiliary metabolic genes were identified, including phoH that may confer vB_EliS-L02 the advantage of regulating phosphate uptake and metabolism under a phosphate-limiting condition. Genomic and phylogenetic analyses indicated that vB_EliS-L02 was most closely related to the genus Lacusarxvirus with low similarity (shared genes < 30%, and average nucleotide sequence identity < 70%), distantly from other reported phages, and could be grouped into a novel viral genus cluster, in this study as Eliscbkvirus. Meanwhile, the genus Eliscbkvirus and Lacusarxvirus stand out from other siphoviral genera and could represent a novel subfamily within Siphoviridae, named Dolichocephalovirinae-II. Being a representative of an understudied viral group with manifold adaptations to the host, phage vB_EliS-L02 could improve our understanding of the virus–host interactions and provide reference information for viral metagenomic analysis in the ocean.

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Section: Resultsmentioning

confidence: 99%

The First Cbk-Like Phage Infecting Erythrobacter, Representing a Novel Siphoviral Genus

Guo

Zou

et al. 2022

Front. Microbiol.

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“…In previous studies, all three genotypes of GCRV were able to infect and proliferate in CIK cells [26][27][28]. Here, we further analyzed the immune response in CIK cells with infection by three GCRVs.…”

Section: Gcrv Infection Triggered Immune Responses In Cik Cellsmentioning

confidence: 89%

“…In previous studies, CIK cells have been identified as susceptible cell lines for three strains of GCRV [26][27][28][29]. We investigated the expression levels of antiviral-related genes at various time points during infection with these three GCRV strains.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptomic Analysis Revealed Potential Differential Mechanisms of Grass Carp Reovirus Pathogenicity

Kong,

Ding,

Yang

et al. 2023

IJMS

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Grass carp reovirus (GCRV), one of the most serious pathogens threatening grass carp (Ctenopharyngodon idella), can lead to grass carp hemorrhagic disease (GCHD). Currently, GCRV can be divided into three genotypes, but the comparison of their pathogenic mechanisms and the host responses remain unclear. In this study, we utilized the Ctenopharyngodon idella kidney (CIK) model infected with GCRV to conduct comparative studies on the three genotypes. We observed a cytopathic effect (CPE) in the GCRV-I and GCRV-III groups, whereas the GCRV-II group did not show any CPE. Moreover, a consistent trend in the mRNA expression levels of antiviral-related genes across all experimental groups of CIK cells was detected via qPCR and further explored through RNA-seq analysis. Importantly, GO/KEGG enrichment analysis showed that GCRV-I, -II, and -III could all activate the immune response in CIK cells, but GCRV-II induced more intense immune responses. Intriguingly, transcriptomic analysis revealed a widespread down-regulation of metabolism processes such as steroid biosynthesis, butanoate metabolism, and N-Glycan biosynthesis in infected CIK cells. Overall, our results reveal the CIK cells showed unique responses in immunity and metabolism in the three genotypes of GCRV infection. These results provide a theoretical basis for understanding the pathogenesis and prevention and control methods of GCRV.

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“…A bioinformatic analysis indicates that the NS31 protein of GCRV shares sequence homology with NS31-like protein from AGCRV and NS4 protein of Green River chinook virus. Similar to the NS31 protein of GCRV, NS31-like proteins from other AqRVs display similar transcriptional activation activity in yeast, which indicates that the function of NS31 in transcriptional activation may be conserved within the genus AqRV 100.…”

mentioning

confidence: 80%

Structure, function and immune evasion strategies of aquareoviruses, with focus on grass carp reovirus

Zhang

Chang

2023

Reviews in Aquaculture

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Aquareoviruses (AqRVs) can infect various wild or cultured bony fishes with serious muscle and viscera haemorrhage syndrome and have caused severe economic losses worldwide. Accumulating evidences have revealed the functions of viral proteins of AqRVs in RNA transcription, virion assembly, cell attachment and/or viral replication. In addition, structural information and transcription mechanism of AqRVs have been in‐depth studied through cryo‐electron microscopy combined with three‐dimensional image reconstruction. Although far from enough, structural and functional studies of AqRVs provide substantial insights into how viral proteins coordinate the replication of AqRVs and escape the host immune response. Grass carp reovirus (GCRV) is the most pathogenic AqRV isolated from aquatic animals. The completion of GCRV lifecycle requires the participation of host proteins. So far, many host factors involved in the entry and replication of GCRV have been identified. Based on the function of viral proteins and the viral lifecycle, many vaccines and antiviral agents have been developed to prevent the replication and infection of GCRV. This review summarises the structural characteristics and the composition of core proteins of AqRVs, as well as synergistic mechanisms of structural proteins on genome transcription and virus entry of AqRVs. In addition, the review highlights our current knowledge for the infection and replication, functions of non‐structural proteins, immune escape mechanisms, as well as the vaccines and antiviral agents of AqRVs, especially for GCRV. A better understanding of structure, function and immune evasion strategies of AqRVs will offer new targets for prevention and control of AqRVs in aquaculture.

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Identification of a potential transcriptional regulator encoded by grass carp reovirus

Cited by 7 publications

References 44 publications

The First Cbk-Like Phage Infecting Erythrobacter, Representing a Novel Siphoviral Genus

The First Cbk-Like Phage Infecting Erythrobacter, Representing a Novel Siphoviral Genus

Comparative Transcriptomic Analysis Revealed Potential Differential Mechanisms of Grass Carp Reovirus Pathogenicity

Structure, function and immune evasion strategies of aquareoviruses, with focus on grass carp reovirus

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