Identification of a potential RNA intermediate for transposition of the LINE-like element I factor in Drosophila melanogaster.

Chaboissier, Marie-Christine; Busseau, Isabelle; Prosser, J.; Finnegan, David J.; Bucheton, Alain

doi:10.1002/j.1460-2075.1990.tb07566.x

Cited by 90 publications

(71 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In situ hybridization experiments were performed on ovaries from 3-d-old SF L and SF H flies according to the method described by Chambeyron et al (2008) using a 2.9-kb I-element antisense riboprobe corresponding to T7 in vitro transcription of a PCR fragment obtained with pSPT19-I plasmid (Chaboissier et al 1990). Briefly, hybridization was performed overnight at 55°C and washing at 55°C.…”

Section: Rna In Situ Hybridizationmentioning

confidence: 99%

piRNA-mediated transgenerational inheritance of an acquired trait

et al. 2012

View full text Add to dashboard Cite

The maintenance of genome integrity is an essential trait to the successful transmission of genetic information. In animal germ cells, piRNAs guide PIWI proteins to silence transposable elements (TEs) in order to maintain genome integrity. In insects, most TE silencing in the germline is achieved by secondary piRNAs that are produced by a feed-forward loop (the pingpong cycle), which requires the piRNA-directed cleavage of two types of RNAs: mRNAs of functional euchromatic TEs and heterochromatic transcripts that contain defective TE sequences. The first cleavage that initiates such an amplification loop remains poorly understood. Taking advantage of the existence of strains that are devoid of functional copies of the LINE-like I-element, we report here that in such Drosophila ovaries, the initiation of a ping-pong cycle is exclusively achieved by secondary I-element piRNAs that are produced in the ovary and deposited in the embryonic germline. This unusual secondary piRNA biogenesis, detected in the absence of functional I-element copies, results from the processing of sense and antisense transcripts of several different defective I-element. Once acquired, for instance after ancestor aging, this capacity to produce heterochromatic-only secondary piRNAs is partially transmitted through generations via maternal piRNAs. Furthermore, such piRNAs acting as ping-pong initiators in a chromatin-independent manner confer to the progeny a high capacity to repress the I-element mobility. Our study explains, at the molecular level, the basis for epigenetic memory of maternal immunity that protects females from hybrid dysgenesis caused by transposition of paternally inherited functional I-element.

show abstract

Section: Rna In Situ Hybridizationmentioning

confidence: 99%

piRNA-mediated transgenerational inheritance of an acquired trait

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Retrotransposon RNA levels are highly variable and tend to be tissue specific in metazoans, with high levels reached only in the germ line in most cases (Chaboissier et al 1990;Branciforte and Martin 1994). Naturally, the abundance of retrotransposon RNAs is very strongly correlated with retrotransposition frequency.…”

Section: Figurementioning

confidence: 99%

The Unusual Phylogenetic Distribution of Retrotransposons: A Hypothesis

Boeke¹

2003

Genome Res.

View full text Add to dashboard Cite

Retrotransposons have proliferated extensively in eukaryotic lineages; the genomes of many animals and plants comprise 50% or more retrotransposon sequences by weight. There are several persuasive arguments that the enzymatic lynchpin of retrotransposon replication, reverse transcriptase (RT), is an ancient enzyme. Moreover, the direct progenitors of retrotransposons are thought to be mobile self-splicing introns that actively propagate themselves via reverse transcription, the group II introns, also known as retrointrons. Retrointrons are represented in modern genomes in very modest numbers, and thus far, only in certain eubacterial and organellar genomes. Archaeal genomes are nearly devoid of RT in any form. In this study, I propose a model to explain this unusual distribution, and rationalize it with the proposed ancient origin of the RT gene. A cap and tail hypothesis is proposed. By this hypothesis, the specialized terminal structures of eukaryotic mRNA provide the ideal molecular environment for the lengthening, evolution, and subsequent massive expansion of highly mobile retrotransposons, leading directly to the retrotransposon-cluttered structure that typifies modern metazoan genomes and the eventual emergence of retroviruses.

show abstract

“…Introns inserted within the I factor are removed during transposition, confirming that an RNA intermediate is involved (28,47). A candidate for this RNA has been identified, the presence and abundance of which correlate with the frequency of transposition (11). It is most abundant in SF ovaries, is rarer in RSF ovaries, is barely detectable in inducer females, and is undetectable in female somatic tissues and in males.…”

mentioning

confidence: 93%

The 5' untranslated region of the I factor, a long interspersed nuclear element-like retrotransposon of Drosophila melanogaster, contains an internal promoter and sequences that regulate expression.

McLean¹,

Bucheton²,

Finnegan³

1993

Mol. Cell. Biol.

View full text Add to dashboard Cite

The I-R system of hybrid dysgenesis in Drosophila melanogaster is controlled by a long interspersed nuclear element-like retroposon, the I factor. Transposition of the I factor occurs at a high frequency only in the ovaries of females produced by crossing males of inducer strains that contain functional I factors with females of reactive strains that lack them. In this study, the 5' untranslated region of the I factor was joined to

show abstract

Identification of a potential RNA intermediate for transposition of the LINE-like element I factor in Drosophila melanogaster.

Cited by 90 publications

References 42 publications

piRNA-mediated transgenerational inheritance of an acquired trait

piRNA-mediated transgenerational inheritance of an acquired trait

The Unusual Phylogenetic Distribution of Retrotransposons: A Hypothesis

The 5' untranslated region of the I factor, a long interspersed nuclear element-like retrotransposon of Drosophila melanogaster, contains an internal promoter and sequences that regulate expression.

Contact Info

Product

Resources

About