Identification of a pollen-specific gene, SlCRK1 (RFK2) in tomato

Kim, Woong Bom; Yi, So Young; Oh, Sang‐Keun; Lim, Chan Ju; Kim, Hyun A; Jang, Hyun A; Lee, Ha Yeon; Park, Youn‐Il; Kwon, Suk‐Yoon

doi:10.1007/s13258-013-0168-3

Cited by 7 publications

(4 citation statements)

References 32 publications

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“…Moreover, the expression levels of pollen development-specific genes were evaluated in WT and SlOFP20 -OE tomato plants. SlCRK1 , a cysteine-rich receptor-like kinase, plays a critical role in pathogen protection and programmed cell death 45 . Pectin methylesterase inhibitor ( SlPMEI ) acts as a key regulator of pectin methylesterase (PME) 46 .…”

Section: Resultsmentioning

confidence: 99%

“…All of these genes showed a trend of downregulation in the transgenic lines, suggesting that SlOFP20 may control the mRNA accumulation of these pollen-specific genes to impact pollen development. In addition, previous studies revealed that many pollen-specific cis-acting elements, including the pollen-specific activation-related elements POLLEN1LELAT52 and the late pollen gene g10-related elements, were enriched in the promoter regions of two pollen-specific genes, SlCRK1 45 and SlPMEI 46 . Promoter-GUS chimeric expression experiments have been used to confirm that the promoters of SlCRK1 45 and SlPMEI 46 exhibit strong pollen-specific activity in the transgenic Arabidopsis and tomato plants.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, previous studies revealed that many pollen-specific cis-acting elements, including the pollen-specific activation-related elements POLLEN1LELAT52 and the late pollen gene g10-related elements, were enriched in the promoter regions of two pollen-specific genes, SlCRK1 45 and SlPMEI 46 . Promoter-GUS chimeric expression experiments have been used to confirm that the promoters of SlCRK1 45 and SlPMEI 46 exhibit strong pollen-specific activity in the transgenic Arabidopsis and tomato plants. In our study, 10 pollen-specific activation-related elements POLLEN1LELAT52 and 10 late pollen gene g10-related elements were found in the SlOFP20 promoter, which further suggests that SlOFP20 may play an important role in pollen development.…”

Section: Discussionmentioning

confidence: 99%

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Overexpression of SlOFP20 affects floral organ and pollen development

Zhou

et al. 2019

Hortic Res

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The OVATE gene was initially identified in tomato and serves as a key regulator of fruit shape. There are 31 OFP members in the tomato genome. However, their roles in tomato growth and reproductive development are largely unknown. Here, we cloned the OFP transcription factor SlOFP20. Tomato plants overexpressing SlOFP20 displayed several phenotypic defects, including an altered floral architecture and fruit shape and reduced male fertility. SlOFP20 overexpression altered the expression levels of some brassinosteroid (BR)-associated genes, implying that SlOFP20 may play a negative role in the BR response, similar to its ortholog OsOFP19 in rice. Moreover, the transcript accumulation of gibberellin (GA)-related genes was significantly affected in the transgenic lines. SlOFP20 may play an important role in the crosstalk between BR and GA. The pollen germination assay suggested that the pollen germination rate of SlOFP20-OE plants was distinctly lower than that of WT plants. In addition, the tomato pollen-associated genes SlCRK1, SlPMEI, LePRK3, SlPRALF, and LAT52 were all suppressed in the transgenic lines. Our data imply that SlOFP20 may affect floral organ and pollen development by modulating BR and GA signaling in tomato.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Overexpression of SlOFP20 affects floral organ and pollen development

Zhou

et al. 2019

Hortic Res

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“…Moreover, less viable pollen grains were observed in the strong overexpression transgenic owers than in the WT owers (data not shown). Subsequently, the relative transcript accumulation of pollen development-related genes SlCRK1 (Kim, et al 2014), SlPRALF (Covey, et al 2010), LePRK3 (Kim, et al 2002), SlPMEI (Kim, et al 2013) were also examined by qRT-PCR assay. Intriguingly, all of these four gene transcripts were consistently reduced in the strong SlMBP22overexpression lines compared to those in the non-transgenic plants (Fig.…”

Section: Overexpression Of Slmbp22 Alters Tomato Ower Morphology and Affects Oral Organ Identity Genesmentioning

confidence: 99%

Overexpression of SlMBP22 in Tomato Affects Flower Morphology, Fruit Set and Development

Jia

Chen

et al. 2021

Preprint

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MADS-domain transcription factors have been clarified as key regulators involved in proper flower and fruit development in angiosperms. Bs genes, as members of the MADS-box subfamily, have been suggested to play an important role during the evolution of the reproductive organs in seed plants. Our knowledge about their effects on reproductive development in fruit crops like tomato (Solanum lycopersicum), however, is still unclear. Here, we found that the overexpression of SlMBP22 (SlMBP22-OE) resulted in considerable alterations regarding floral morphology, and affected the expression levels of several floral homeotic genes. Further analysis by yeast-two-hybrid assays demonstrated that SlMBP22 could form dimers with class A protein MACROCALYX (MC) and with SEPALLATA (SEP) floral homeotic proteins TM5 and TM29, respectively. In addition, pollen viability and cross-fertilization assays suggested that the defect in female reproductive development was responsible for infertility phenotype observed in the strong overexpression transgenic plants. The mild overexpression transgenic fruits were reduced in size, as a result of reduced cell expansion, rather than impaired cell division. Additionally, overexpression of SlMBP22 in tomato not only affected proanthocyanidin (PA) accumulation but also altered seed dormancy. Taken together, these findings may provide new insights into the knowledge of Bs MADS-box genes in flower and fruit development in tomato.

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Cysteine-rich receptor-like kinases and stress response in plants

Shumayla

Upadhyay

2023

Plant Receptor-Like Kinases

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Identification of a pollen-specific gene, SlCRK1 (RFK2) in tomato

Cited by 7 publications

References 32 publications

Overexpression of SlOFP20 affects floral organ and pollen development

Overexpression of SlOFP20 affects floral organ and pollen development

Overexpression of SlMBP22 in Tomato Affects Flower Morphology, Fruit Set and Development

Cysteine-rich receptor-like kinases and stress response in plants

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