Identification of a peroxisomal-targeted aldolase involved in chlorophyll biosynthesis and sugar metabolism in rice

Zhang, Fei; Zhang, Pan; Wang, Shouchuang; Qu, Lianghuan; Liu, Xianqing; Luo, Jie

doi:10.1016/j.plantsci.2016.06.017

Cited by 14 publications

(13 citation statements)

References 48 publications

(55 reference statements)

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“…Liu found that rice mutant OsNOA1 / RIF1 RNAi , its seedlings showed chlorosis, has a decreased pigment content and photosystem II efficiency. In this study, FBA1 protein abundance and the gene expression levels were down-regulated in W7–2 (Table S5, Figure N), which would lead to an inability to accumulate large amounts of sucrose (Figure C) and interfere with Chl biosynthesis . Further research is needed to determine whether carbohydrate metabolism regulates chlorophyll biosynthesis.…”

Section: Discussionmentioning

confidence: 79%

Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai (Brassica campestris L.) Responding to Cold Acclimation

et al. 2019

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Chlorophyll is a vital photosynthetic pigment that plays a key role in plant development, participating in light energy capture and energy conversion. In this study, a novel wucai (Brassica campestris L.) germplasm with green outer leaves and yellow inner leaves at the adult stage (W7−2) was used to examine chlorophyll metabolism response to cold acclimation. A green leaf wucai genotype without leaf color changes named W7−1 was selected as the control to evaluate the chlorophyll metabolism changes of W7−2. Compared to W7−1, the contents of chlorophyll a (Chl a) and chlorophyll b (Chl b) in W7−2 were significantly reduced at five developmental stages (13, 21, 29, 37, and 45 days after planting (DAP)). An iTRAQ-based quantitative proteomic analysis was carried out at 21 and 29 DAP according to the leaf color changes in both of genotypes. 1409 proteins were identified, while 218 of them displayed differential accumulations between W7−2 and W7−1 during the two developmental stages. The differentially expressed proteins (DEPs) mainly assigned to chlorophyll biosynthesis, photosynthesis, carbohydrate metabolism, ribosome metabolism and posttranslational modification. Among these DEPs, NADPH-protochlorophyllide oxidoreductase (PORB) and Mgprotoporphyrin IX chelatase 1 (CHLI1) were the key enzymes participating in chlorophyll (Chl) biosynthesis, which was down-regulated at 21 DAP and up-regulated at 29 DAP in W7−2 compared with W7−1, respectively. The expression analysis of genes of three subunits of Mg-chelatase (CHLI1, CHLD, and CHLH), Genomes Uncoupled 4 (GUN4), and Thioredoxin (TRX3) associated with chlorophyll metabolism also displayed significant down-regulation in W7−2. In particular, PORB showed significant up-regulation in W7−2, significantly affecting chlorophyll biosynthesis. Additionally, differences in chlorophyll metabolism between W7−2 and W7−1 were in terms of altered photosynthesis, carbohydrate, and energy metabolism. We found that the transcription levels of most photosynthesis proteins showed significantly lower levels, and the genes expression level, associated with carbohydrate and energy metabolism, were lower in W7−2 than in W7−1. Therefore, the present study results help understand the physiological and molecular mechanisms underlying leaf coloring responding to cold acclimation.

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Section: Discussionmentioning

confidence: 79%

Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai (Brassica campestris L.) Responding to Cold Acclimation

et al. 2019

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“…The previous studies revealed that the sucrose content was regulated positively (synthesis) and negatively (degradation) by genes involved in sucrose metabolism [ 56 , 57 ]. For the synthesis of sucrose, it had been reported that SPP [ 15 ], FBA [ 58 ], PGI [ 59 ], and HK [ 60 ] participated in sucrose synthesis and that their up-regulated expression could promote sucrose accumulation. In the present study, genes arahy.7JQX15 (PGI), arahy.05JDR6 (SPP), arahy.8L28UA (HK), arahy.SFW5N9 (FBA), and arahy.LI5GRW (FBA), exhibited high levels of expression in ICG 12625, which, in turn, promoted the synthesis of sucrose and contributed to the final sucrose content in the peanut seed.…”

Section: Discussionmentioning

confidence: 99%

Key Regulators of Sucrose Metabolism Identified through Comprehensive Comparative Transcriptome Analysis in Peanuts

Huang

Liu

et al. 2021

IJMS

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Sucrose content is a crucial indicator of quality and flavor in peanut seed, and there is a lack of clarity on the molecular basis of sucrose metabolism in peanut seed. In this context, we performed a comprehensive comparative transcriptome study on the samples collected at seven seed development stages between a high-sucrose content variety (ICG 12625) and a low-sucrose content variety (Zhonghua 10). The transcriptome analysis identified a total of 8334 genes exhibiting significantly different abundances between the high- and low-sucrose varieties. We identified 28 differentially expressed genes (DEGs) involved in sucrose metabolism in peanut and 12 of these encoded sugars will eventually be exported transporters (SWEETs). The remaining 16 genes encoded enzymes, such as cell wall invertase (CWIN), vacuolar invertase (VIN), cytoplasmic invertase (CIN), cytosolic fructose-bisphosphate aldolase (FBA), cytosolic fructose-1,6-bisphosphate phosphatase (FBP), sucrose synthase (SUS), cytosolic phosphoglucose isomerase (PGI), hexokinase (HK), and sucrose-phosphate phosphatase (SPP). The weighted gene co-expression network analysis (WGCNA) identified seven genes encoding key enzymes (CIN, FBA, FBP, HK, and SPP), three SWEET genes, and 90 transcription factors (TFs) showing a high correlation with sucrose content. Furthermore, upon validation, six of these genes were successfully verified as exhibiting higher expression in high-sucrose recombinant inbred lines (RILs). Our study suggested the key roles of the high expression of SWEETs and enzymes in sucrose synthesis making the genotype ICG 12625 sucrose-rich. This study also provided insights into the molecular basis of sucrose metabolism during seed development and facilitated exploring key candidate genes and molecular breeding for sucrose content in peanuts.

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“…Integrated analysis between LCR genes and DEGs identi ed in Hwaseong and CR2002 (Shim et al 2020) Abbreviations CD: constant dark; Chl: chlorophyll; DEG: differentially expressed gene; GMP: genes in a speci c metabolic pathway; KEGG: Kyoto encyclopedia of genes and genomes; LCR: leaf color-related; LHC: light-harvesting complex; mRF: mutant rich factor; PCM: porphyrin and chlorophyll metabolism; Phy: phytochrome; PIF: phytochrome interacting factor; PIL: rice phytochrome-interacting factor-like; RF: rich factor. (Gao et al, 2012) OsHY2 (Yoshitake et al, 2015) OsSHMT1 (Wu et al, 2015) OsAK1 (Wei et al, 2017) OsHYR (Ambavaram et al, 2011) OsSIG2A (Yu et al, 2019) OsAL1 (Zhang et al, 2016b) OsIspE (Chen et al, 2018) OsSLL1 (Zhang et al, 2009) OsAL2 (Liu et al, 2016a) OsIspF (Huang et al, 2017) OsSPP (Yue et al, 2010) OsALD-Y (Zhang et al, 2016a) OsKS2 (Ji et al, 2013) OsSRP43 (Lv et al, 2015) OsALS3 (Lin et al, 2015a) OsLAS (Zhang et al, 2017a) OsSRP54 (Zhang et al, 2013a) OsAM1 (Sheng et al, 2014) OsLC7 (Chen et al, 2016b) OsSWL1 (Tsugane et al, 2014) OsAPX2 (Zhang et al, 2013b) OsLCY (Fang et al, 2008) OsTCD10 (Wu et al, 2016b) OsARVL4 (Wang et al, 2016c) OsLhca4 (Yamatani et al, 2018) OsTCD11 (Wang et al, 2017a) OsASL1 (Gong et al, 2013) OsLMM24 (Zhang et al, 2019) OsTCD5 (Wang et al, 2016f) OsASL2 (Lin et al, 2015b) OsLS1 (Qiu et al, 2019) OsTCM1 (Lin et al, 2018b) OsBE1 OsLYL1 (Zhou et al, 2013b) OsTCM5…”

Section: Supplementary Informationmentioning

confidence: 99%

Integrated analyses of rice dark response and leaf color regulation reveal links with porphyrin and chlorophyll metabolism

Wang

Chen

Zhu

et al. 2020

Preprint

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Background Light is a key regulatory signal for rice growth and development. Under dark stress, rice shows leaf yellowing. Whole genome transcriptomic analysis will identify differentially expressed genes (DEG) in dark-treated rice seedlings and DEG-enriched metabolic pathways. Rice leaf color is an essential agronomic trait. Traditional genetic experiments have reported over a hundred of leaf color-related (LCR) genes and some of them were also regulated by light signal. Thus, an integrated analysis for the two set of data will be helpful for illustration of the mechanism for both dark-response and leaf color regulation. Results Transcriptome changes in response to dark treatment were surveyed by RNA-Seq analysis. About 13,115 DEGs were identified. One hundred and fifty rice LCR genes were collected. It was found that 102 LCR genes (68.0%) were also dark-response DEGs, which suggests an overlap between dark response and LCR networks. Fifty DEG overlapped LCR genes was associated with chloroplast development. KEGG analysis revealed enrichment of LCR genes in porphyrin and chlorophyll metabolism (PCM) (18/44, 40.9%). Of the 18 LCR genes in the PCM pathway, 15 were dark-response DEGs (83.3%). More interestingly, most of them are involved in a central PCM sub-pathway, chlorophyll biosynthesis. Conclusions Integrated analysis for dark stress-response and leaf color regulation identified the correlation between the two processes and mutually supported evidences were obtained. It was found that PCM pathway, particularly chlorophyll biosynthesis process, is a core component of the overlap and plays important roles in rice LCR and dark stress-response. This study provides important clues for identifying additional LCR genes, understanding the mechanisms of dark response and leaf color regulation.

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Identification of a peroxisomal-targeted aldolase involved in chlorophyll biosynthesis and sugar metabolism in rice

Cited by 14 publications

References 48 publications

Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai (Brassica campestris L.) Responding to Cold Acclimation

Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai (Brassica campestris L.) Responding to Cold Acclimation

Key Regulators of Sucrose Metabolism Identified through Comprehensive Comparative Transcriptome Analysis in Peanuts

Integrated analyses of rice dark response and leaf color regulation reveal links with porphyrin and chlorophyll metabolism

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