Identification of a novel thylakoid protein gene involved in cold acclimation in cyanobacteria

Li, Weizhi; Gao, Hong; Yin, Chuntao; Xu, Xudong

doi:10.1099/mic.0.060038-0

Cited by 9 publications

(9 citation statements)

References 41 publications

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“…In this investigation, our research group predicted temperature-responsive genes by metabolic pathway positioning through KEGG analysis, and selected part of the genes for further validation. Some of the selected genes comprised a large proportion of the total differentially expressed genes, and were possible temperature-responsive genes reported in previous reports [22,23,24], while the others were the up-regulated proteins or solely visible spots at 45°C or 15°C, while not visible at 35°C.…”

Section: Methodsmentioning

confidence: 94%

“…For example, Hsp60 can refold the Rubisco enzyme of cyanobacteria and malate dehydrogenase of mitochondrion in vitro [36]. This type of molecular chaperone usually plays an important role in protein migration, translation mechanisms on thylakoid membrane surfaces and improving the fluidity of membranes in order to maintain metabolic activity at low temperatures [22,37]. The up-regulation of the molecular chaperone, Hsp60/GroEL, was also found on exposure to low temperature.…”

Section: Discussionmentioning

confidence: 99%

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Proteomic Analysis and qRT-PCR Verification of Temperature Response to Arthrospira (Spirulina) platensis

et al. 2013

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Arthrospira (Spirulina) platensis (ASP) is a representative filamentous, non-N2-fixing cyanobacterium that has great potential to enhance the food supply and possesses several valuable physiological features. ASP tolerates high and low temperatures along with highly alkaline and salty environments, and can strongly resist oxidation and irradiation. Based on genomic sequencing of ASP, we compared the protein expression profiles of this organism under different temperature conditions (15°C, 35°Cand 45°C) using 2-DE and peptide mass fingerprinting techniques. A total of 122 proteins having a significant differential expression response to temperature were retrieved. Of the positively expressed proteins, the homologies of 116 ASP proteins were found in Arthrospira (81 proteins in Arthrospira platensis str. Paraca and 35 in Arthrospira maxima CS-328). The other 6 proteins have high homology with other microorganisms. We classified the 122 differentially expressed positive proteins into 14 functions using the COG database, and characterized their respective KEGG metabolism pathways. The results demonstrated that these differentially expressed proteins are mainly involved in post-translational modification (protein turnover, chaperones), energy metabolism (photosynthesis, respiratory electron transport), translation (ribosomal structure and biogenesis) and carbohydrate transport and metabolism. Others proteins were related to amino acid transport and metabolism, cell envelope biogenesis, coenzyme metabolism and signal transduction mechanisms. Results implied that these proteins can perform predictable roles in rendering ASP resistance against low and high temperatures. Subsequently, we determined the transcription level of 38 genes in vivo in response to temperature and identified them by qRT-PCR. We found that the 26 differentially expressed proteins, representing 68.4% of the total target genes, maintained consistency between transcription and translation levels. The remaining 12 genes showed inconsistent protein expression with transcription level and accounted for 31.6% of the total target genes.

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Section: Methodsmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis and qRT-PCR Verification of Temperature Response to Arthrospira (Spirulina) platensis

et al. 2013

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“…The upregulation of ftsH observed in our study under chill/light stress after 24 h (which was prolonged in the following days) suggests that in AMU-DH-30 ftsH supports the assembly of protein complexes in the photosynthetic electron-transport pathways. Other genes that are required to support the photosynthetic activity at a low temperature, crucial in the response to chill/light stress belong to ccr (cyanobacterial cold resistance genes) [15,16]. Their expression can be influenced by Rbp proteins [38] as discussed above.…”

Section: Discussionmentioning

confidence: 99%

“…The crucial role of such mechanisms in ACLT has been documented mainly for the laboratory strain Synechocystis sp. PCC 6803 [14][15][16] and Microcystis sp. [17].…”

Section: Introductionmentioning

confidence: 99%

Different Gene Expression Response of Polish and Australian Raphidiopsis raciborskii Strains to the Chill/Light Stress

et al. 2020

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R. raciborskii is known for growing under wide ranges of temperature and light. In temperate regions, however, low temperature and high light may serve as a stressful condition for invading tropical populations. The genetic basis of R. raciborskii’s adaptation to this combination of stresses are unknown. In this study, the growth rate and the expression of genes that may be crucial in the response to the chill/light stress of two R. raciborskii strains (differing in their climatic origin and toxicity) exposed to low temperature and high light were examined. Results showed that AMU-DH-30, a non CYN (cylindrospermopsin) producing strain isolated from the temperate region, exhibited under stress the upregulation of genes involved in the protein translation (rbp1, nusG, hflX), membrane fluidity (desA), photosynthetic activity (ccr2 and ftsH), and the accumulation of compatible solutes (asd). In contrast, a CYN producing Australian strain CS-505 was not able to adapt quickly and to continue growth during stress conditions. Intriguingly, CS-505 and AMU-DH-30 had a similar ability to resume growth when the stress conditions subsided. Moreover, in strain CS-505 the cyrB gene was significantly upregulated under the stress conditions. The presented results shed new light on the possible mechanisms involved in the response of R.raciborskii to chill/light stress.

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“…Studies on how cyanobacteria adapt to low temperatures can be roughly divided into two categories: (i) cold acclimation of cells exposed to a suboptimal growth temperature, such as 22 to 24°C (9,10) or 15°C (11) and (ii) survival under long-term (8 to 10 days) chill (5°C)-light stress as a simulation of overwintering (7,8,12). In previous reports, we showed that ␣-tocopherol, which is membrane localized, is essential for the acquired chill-light tolerance (ACLT) of cyanobacteria (7) and that accumulation of RNAbinding protein 1 (Rbp1) leads to the formation of overwintering capability of cyanobacteria (8,12).…”

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confidence: 99%

Role of Spermidine in Overwintering of Cyanobacteria

Zhu

Yin

et al. 2015

J Bacteriol

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Polyamines are found in all groups of cyanobacteria, but their role in environmental adaptation has been barely investigated. In Synechocystis sp. strain PCC 6803, inactivation of spermidine synthesis genes significantly reduced the survivability under chill (5°C)-light stress, and the survivability could be restored by addition of spermidine. To analyze the effects of spermidine on gene expression at 5°C, lacZ was expressed from the promoter of carboxy(nor)spermidine decarboxylase gene (CASDC) in Synechocystis. Synechocystis 6803::P CASDC -lacZ pretreated at 15°C showed a high level of LacZ activity for a long period of time at 5°C; without the pretreatment or with protein synthesis inhibited at 5°C, the enzyme activity gradually decreased. In a spermidine-minus mutant harboring P CASDC -lacZ, lacZ showed an expression pattern as if protein synthesis were inhibited at 5°C, even though the stability of its mRNA increased. Four other genes, including rpoA that encodes the ␣ subunit of RNA polymerase, showed similar expression patterns. The chill-light stress led to a rapid increase of protein carbonylation in Synechocystis. The protein carbonylation then quickly returned to the background level in the wild type but continued to slowly increase in the spermidine-minus mutant. Our results indicate that spermidine promotes gene expression and replacement of damaged proteins in cyanobacteria under the chill-light stress in winter. IMPORTANCEOutbreak of cyanobacterial blooms in freshwater lakes is a worldwide environmental problem. In the annual cycle of bloomforming cyanobacteria, overwintering is the least understood stage. Survival of Synechocystis sp. strain PCC 6803 under longterm chill (5°C)-light stress has been established as a model for molecular studies on overwintering of cyanobacteria. Here, we show that spermidine, the most common polyamine in cyanobacteria, promotes the survivability of Synechocystis under longterm chill-light stress and that the physiological function is based on its effects on gene expression and recovery from protein damage. This is the first report on the role of polyamines in survival of overwintering cyanobacteria. We also analyzed spermidine synthesis pathways in cyanobacteria on the basis of bioinformatic and experimental data.

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Identification of a novel thylakoid protein gene involved in cold acclimation in cyanobacteria

Cited by 9 publications

References 41 publications

Proteomic Analysis and qRT-PCR Verification of Temperature Response to Arthrospira (Spirulina) platensis

Proteomic Analysis and qRT-PCR Verification of Temperature Response to Arthrospira (Spirulina) platensis

Different Gene Expression Response of Polish and Australian Raphidiopsis raciborskii Strains to the Chill/Light Stress

Role of Spermidine in Overwintering of Cyanobacteria

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