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2007
DOI: 10.1007/s10719-007-9080-x
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Identification of a novel sugar 5,7-diacetamido-8-amino-3,5,7,8,9-pentadeoxy-d-glycero-d-galacto-non-2-ulosonic acid present in the lipooligosaccharide of Vibrio parahaemolyticus O3:K6

Abstract: A novel sugar, 5,7-diacetamido-8-amino-3,5,7,8,9-pentadeoxy-D-glycero-D-galacto-non-2-ulosonic acid (NonlA), has been identified as a component of the oligosaccharide (OS) isolated from the lipooligosaccharide (LOS) of the emerging strain of Vibrio parahaemolyticus O3:K6 associated with a global pandemic. In the present study we report the identification and characterization of this novel sugar present in the OS of V. parahaemolyticus O3:K6, using chemical analysis, NMR spectroscopy and mass spectrometry.

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Cited by 3 publications
(2 citation statements)
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“…The most popular methods are the formation of 2-butyl glycosides by reaction with 2-butanol [169] which has been cited in about 600 papers, and 2-octyl glycosides formed by reaction with 2-octanol [170] cited in about 300 papers, being often complementary of the first. Among the numerous applications which have been published, the following are mentioned as examples: polysaccharide from microrganisms [175][176][177][178], algae [179,180], marine microorganisms [181], medicinal fungus [182], cuttlefish [183], wheat germ [184], edible vegetables [185] and lactic bacteria [186]. A method based in reductive amination [173] was developed for the study of algal galactans and is used by groups working in this field [187][188][189].…”
Section: Formation Of Diastereomersmentioning
confidence: 99%
“…The most popular methods are the formation of 2-butyl glycosides by reaction with 2-butanol [169] which has been cited in about 600 papers, and 2-octyl glycosides formed by reaction with 2-octanol [170] cited in about 300 papers, being often complementary of the first. Among the numerous applications which have been published, the following are mentioned as examples: polysaccharide from microrganisms [175][176][177][178], algae [179,180], marine microorganisms [181], medicinal fungus [182], cuttlefish [183], wheat germ [184], edible vegetables [185] and lactic bacteria [186]. A method based in reductive amination [173] was developed for the study of algal galactans and is used by groups working in this field [187][188][189].…”
Section: Formation Of Diastereomersmentioning
confidence: 99%
“…Endoglycoceramidase was used by Meyer et al (2007) to release glycans from Schistosoma mansoni glycosphingolipids for analysis by MALDI-TOF and to show that they were recognized by the C-type lectin, L-SIGN. A novel (Mazumder, et al, 2008) Xanthomonas Campestris LPS L-, R-TOF (THAP (intact LPS)), DHB carbohydrates, GLC, GC/MS, NMR Acylation and phosphorylation pattern of lipid A shown to strongly influence ability to trigger the innate immune response in Arabidopsis 1 Format (not all items present): MALDI method (matrix), other methods.…”
Section: R-tof (Thap) Gc/msmentioning
confidence: 99%