Identification of a novel role for <i>Drosophila </i><scp>MESR</scp>4 in lipid metabolism

Tsuda‐Sakurai, Kayoko; Seong, Ki-Hyeon; Horiuchi, Junjiro; Aigaki, Toshiro; Tsuda, Manabu

doi:10.1111/gtc.12221

Cited by 9 publications

(9 citation statements)

References 24 publications

(38 reference statements)

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“…Overexpression of MESR4 alone causes various defects in the adult fly morphology, such as loss of macrocheatae and dorsal thorax cleft (Abdelilah-Seyfried et al, 2000;Pena-Rangel et al, 2002). Recently, it has been shown that overexpression of MESR4 promoted catabolism of neutral fat in Drosophila fat storage organ called fat bodies to release free fatty acids (Tsuda-Sakurai et al, 2015). RNAi-mediated knockdown experiments suggested a role in innate immunity and cellular response to hypoxia (Cheng et al, 2005;Lee et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…y w 67c23 (referred to as y w) was used as a control. MESR4 LA00390 has an insertion of P{Mae-UAS.6.11} in the MESR4 gene (Tsuda-Sakurai et al, 2015). A green balancer, CyO chromosome bearing kr-GAL4 and UAS-GFP S65T transgenes (Casso et al, 2000) was used to identify the genotype of embryos.…”

Section: Fly Strainsmentioning

confidence: 99%

“…Western blot was carried out according to a standard method using a rabbit anti-MESR4 antibody (Tsuda-Sakurai et al, 2015), and an HRP-conjugated anti-rabbit IgG (GE Healthcare) at dilution of 1:1,000. Signals were detected using ECL-plus reagents (GE Healthcare).…”

Section: Mapping Of P-elementsmentioning

confidence: 99%

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The plant homeodomain finger protein MESR4 is essential for embryonic development in Drosophila

Seong

Tsuda

Tsuda‐Sakurai

et al. 2015

Genesis

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Misexpression Suppressor of Ras 4 (MESR4), a plant homeodomain (PHD) finger protein with nine zinc-finger motifs has been implicated in various biological processes including the regulation of fat storage and innate immunity in Drosophila. However, the role of MESR4 in the context of development remains unclear. Here it is shown that MESR4 is a nuclear protein essential for embryonic development. Immunostaining of polytene chromosomes using anti-MESR4 antibody revealed that MESR4 binds to numerous bands along the chromosome arms. The most intense signal was detected at the 39E-F region, which is known to contain the histone gene cluster. P-element insertions in the MESR4 locus, which were homozygous lethal during embryogenesis with defects in ventral ectoderm formation and head encapsulation was identified. In the mutant embryos, expression of Fasciclin 3 (Fas3), an EGFR signal target gene was greatly reduced, and the level of EGFR signal-dependent double phosphorylated ERK (dp-ERK) remained low. However, in the context of wing vein formation, genetic interaction experiments suggested that MESR4 is involved in the EGFR signaling as a negative regulator. These results suggested that MESR4 is a novel chromatin-binding protein required for proper expression of genes including those regulated by the EGFR signaling pathway during development. genesis 53:701-708, 2015. © 2015 Wiley Periodicals, Inc.

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Section: Resultsmentioning

confidence: 99%

Section: Fly Strainsmentioning

confidence: 99%

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The plant homeodomain finger protein MESR4 is essential for embryonic development in Drosophila

Seong

Tsuda

Tsuda‐Sakurai

et al. 2015

Genesis

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“…The float buoyancy experiments were conducted essentially as described by Reis et al 2010 and Reis 2016 using 30-50 larvae per sample, with initial results confirmed by the method of Hazegh and Reis (2016) . A relationship between the percentage of larvae floating in a buoyancy assay and the percent body fat of the larvae has been established ( Reis et al, 2010 ; Tsuda-Sakurai et al, 2015 ). Thus, in this study obesity is defined by the relative proportion of the larvae floating in the buoyancy assay.…”

Section: Methodsmentioning

confidence: 99%

Regulated inositol synthesis is critical for balanced metabolism and development in Drosophila melanogaster

et al. 2021

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Myo-inositol is a precursor of the membrane phospholipid, phosphatidylinositol (PI). It is involved in many essential cellular processes including signal transduction, energy metabolism, endoplasmic reticulum stress, and osmoregulation. Inositol is synthesized from glucose-6-phosphate by myo-inositol-3-phosphate synthase (MIPSp). The Drosophila melanogaster Inos gene encodes MIPSp. Abnormalities in myo-inositol metabolism have been implicated in type 2 diabetes, cancer, and neurodegenerative disorders. Obesity and high blood (hemolymph) glucose are two hallmarks of diabetes, which can be induced in Drosophila melanogaster third-instar larvae by high-sucrose diets. This study shows that dietary inositol reduces the obese-like and high-hemolymph glucose phenotypes of third-instar larvae fed high-sucrose diets. Furthermore, this study demonstrates Inos mRNA regulation by dietary inositol; when more inositol is provided there is less Inos mRNA. Third-instar larvae with dysregulated high levels of Inos mRNA and MIPSp show dramatic reductions of the obese-like and high-hemolymph glucose phenotypes. These strains, however, also display developmental defects and pupal lethality. The few individuals that eclose die within two days with striking defects: structural alterations of the wings and legs, and heads lacking proboscises. This study is an exciting extension of the use of Drosophila melanogaster as a model organism for exploring the junction of development and metabolism.

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“…At the cellular level, obesity can be characterized via quantification of the fat body lipid droplet (LD) size and number (Grönke et al, 2005;Musselman et al, 2011). Some researchers have taken advantage of the altered ratio of fat-to-lean body mass to identify genotypes with increased fat content using buoyancy-based genetic screens (Reis et al, 2010;Tsuda-Sakurai et al, 2015). Lipogenesis can be further characterized by the incorporation of labeled carbons from the diet into various lipid classes using mass spectrometry .…”

Section: Introductionmentioning

confidence: 99%

Drosophilaas a model to study obesity and metabolic disease

Musselman

Kühnlein

2018

Journal of Experimental Biology

175

130

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Excess adipose fat accumulation, or obesity, is a growing problem worldwide in terms of both the rate of incidence and the severity of obesity-associated metabolic disease. Adipose tissue evolved in animals as a specialized dynamic lipid storage depot: adipose cells synthesize fat (a process called lipogenesis) when energy is plentiful and mobilize stored fat (a process called lipolysis) when energy is needed. When a disruption of lipid homeostasis favors increased fat synthesis and storage with little turnover owing to genetic predisposition, overnutrition or sedentary living, complications such as diabetes and cardiovascular disease are more likely to arise. The vinegar fly Drosophila melanogaster (Diptera: Drosophilidae) is used as a model to better understand the mechanisms governing fat metabolism and distribution. Flies offer a wealth of paradigms with which to study the regulation and physiological effects of fat accumulation. Obese flies accumulate triacylglycerols in the fat body, an organ similar to mammalian adipose tissue, which specializes in lipid storage and catabolism. Discoveries in Drosophila have ranged from endocrine hormones that control obesity to subcellular mechanisms that regulate lipogenesis and lipolysis, many of which are evolutionarily conserved. Furthermore, obese flies exhibit pathophysiological complications, including hyperglycemia, reduced longevity and cardiovascular functionsimilar to those observed in obese humans. Here, we review some of the salient features of the fly that enable researchers to study the contributions of feeding, absorption, distribution and the metabolism of lipids to systemic physiology.

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Identification of a novel role for Drosophila MESR4 in lipid metabolism

Cited by 9 publications

References 24 publications

The plant homeodomain finger protein MESR4 is essential for embryonic development in Drosophila

The plant homeodomain finger protein MESR4 is essential for embryonic development in Drosophila

Regulated inositol synthesis is critical for balanced metabolism and development in Drosophila melanogaster

Drosophilaas a model to study obesity and metabolic disease

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