Identification of a Novel Mutation in FAD2B from a Peanut EMS Mutant with Elevated Oleate Content

FAD2B from a Peanut Mutant with High Oleic Acid Content Was Not Completely Dysfunctional

“…Oleate content of the peanut EMS mutant in the report of Fang et al [12] was only 66.4 %, which is significantly lower than that in the sodium azide mutant from the present study (higher than 72 %). Interestingly, even though both of the FAD2A and FAD2B genes from the EMS mutant lost their function, the oleate content in the EMS mutant was not high enough.…”

“…PCR reaction mixtures and thermal cycling profiles were the same as those described by Fang et al [12]. PCR products were purified, cloned, and sequenced.…”

“…Then the collection was resuspended in 50 mL liquid inducing medium and cultured at 30°C with agitation (220 rpm) for 48 h. Cells were again collected by centrifugation at 1,500 g 4°C for 5 min, then vacuum-dried and ground into a fine powder in liquid nitrogen. About 20 μg of the powder samples were used to analyze fatty acids by gas chromatograph-mass spectroscopy (GC-MS) [12].…”

Mutagenesis: A Useful Tool for the Genetic Improvement of the Cultivated Peanut (Arachis hypogaea L.)

“…We have reported a high oleate EMS mutant of LF 2 (2010 seed no. : E2-4-83-12), an output of the present study, that had dysfunctional mutated FAD2A (G448A in the coding region) and FAD2B (C313T in the coding region) [4]. Despite the uncertainty in quality traits, selection in M2 seeds is still necessary as it helps to reduce the population size of M2 plant and hence M3 seed generations.…”

“…In developing countries, a large portion of peanuts are crushed for edible oil [3]. Food uses of peanut are predominant in developed nations, where high oleate, high protein and reduced fat peanuts are most preferred, as high oleate not only means better keeping quality, but also brings about multiple health benefits, for example, reduced risk of cardiovascular disease, increased sensitivity to insulin, preventative effects on tumorigenesis, and amelioration of some inflammatory diseases [3,4].…”