Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in <i>Oryza sativa</i>

Jia, Liqiang; Wu, Zhongchang; Xi, Hu; Carrie, Chris; Zheng, Lei; Whelan, James; Wu, Yunrong; Wang, Shoufeng; Wu, Ping

doi:10.1111/j.1469-8137.2010.03513.x

Cited by 37 publications

(26 citation statements)

References 43 publications

(90 reference statements)

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“…The results showed that the expression of all these genes was upregulated in osabcb14 mutants (Figures a, S4a). These genes were reported to be upregulated under –Fe (Jia et al ., ; Qi et al ., ). Consistent with this finding, qRT‐PCR analysis here showed that all these genes were induced in NIP and osabcb14 mutants under –Fe, but that the induced levels in osabcb14 mutants were significantly lower than in NIP (Figures b, S4b).…”

Section: Resultsmentioning

confidence: 99%

“…Rice seedlings grown in hydroponics to 2 weeks of age and peeled rice seeds were analyzed to determine their metal ion concentrations with inductively coupled plasma mass spectrometry (Agilent 7500ce; Agilent Technologies, http://www.home.agi lent.com), as previously described (Jia et al, 2011). Five biological replicates were performed for each sample.…”

Section: Metal Ion Concentrations Assaymentioning

confidence: 99%

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OsABCB14 functions in auxin transport and iron homeostasis in rice (Oryza sativa L.)

Zhang

Guo

et al. 2014

The Plant Journal

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These authors contributed equally to this work. SUMMARYMembers of the ATP Binding Cassette B/Multidrug-Resistance/P-glyco-protein (ABCB/MDR/PGP) subfamily were shown to function primarily in Oryza sativa (rice) auxin transport; however, none of the rice ABCB transporters have been functionally characterized. Here, we describe that a knock-down of OsABCB14 confers decreased auxin concentrations and polar auxin transport rates, conferring insensitivity to 2,4-dichlorophenoxyacetic acid (2,4-D) and indole-3-acetic acid (IAA). OsABCB14 displays enhanced specific auxin influx activity in yeast and protoplasts prepared from rice knock-down alleles. OsABCB14 is localized at the plasma membrane, pointing to an important directionality under physiological conditions. osabcb14 mutants were surprisingly found to be insensitive to iron deficiency treatment (-Fe). Their Fe concentration is higher and upregulation of Fe deficiency-responsive genes is lower in osabcb14 mutants than in wild-type rice (Nipponbare, NIP). Taken together, our results strongly support the role of OsABCB14 as an auxin influx transporter involved in Fe homeostasis. The functional characterization of OsABCB14 provides insights in monocot auxin transport and its relationship to Fe nutrition.

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Section: Resultsmentioning

confidence: 99%

Section: Metal Ion Concentrations Assaymentioning

confidence: 99%

OsABCB14 functions in auxin transport and iron homeostasis in rice (Oryza sativa L.)

Zhang

Guo

et al. 2014

The Plant Journal

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“…The EMS-mutated mutant library was generated using the indica rice cv Kasalath (Kas, as described previously in Jia et al (2011), and then screened for mutants with abnormal crown root development. The lcrn1 Kas mutant was isolated and crossed with a japonica rice cultivar, Hei Jing2 (HJ2), with a shorter growth period.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

OsSPL3, an SBP-Domain Protein, Regulates Crown Root Development in Rice

et al. 2019

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The major root system of cereals consists of crown roots (or adventitious roots), which are important for anchoring plants in the soil and for water and nutrient uptake. However, the molecular basis of crown root formation is largely unknown. Here, we isolated a rice (Oryza sativa) mutant with fewer crown roots, named lower crown root number1 (lcrn1). Map-based cloning revealed that lcrn1 is caused by a mutation of a putative transcription factor-coding gene, O. sativa SQUAMOSA PROMOTER BINDING PROTEIN-LIKE3 (OsSPL3). We demonstrate that the point mutation in lcrn1 perturbs theO. sativa microRNA156 (OsmiR156)-directed cleavage of OsSPL3 transcripts, resulting in the mutant phenotype. Chromatin immunoprecipitation sequencing assays of OsSPL3 binding sites and RNA sequencing of differentially expressed transcripts in lcrn1 further identified potential direct targets of OsSPL3 in basal nodes, including a MADS-box transcription factor, OsMADS50. OsMADS50-overexpressing plants produced fewer crown roots, phenocopying lcrn1, while knocking out OsMADS50 in the lcrn1 background reversed this phenotype. We also show that OsSPL12, another OsmiR156 target gene, regulates OsMADS50 and crown root development. Taken together, our findings suggest a novel regulatory pathway in which the OsmiR156-OsSPL3/OsSPL12 module directly activates OsMADS50 in the node to regulate crown root development in rice.

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“…To date, four short-root genes, OsGNA1, GLR3.1, OsCyt-inv1 and SPR1 have been cloned [11][12][13][14]. osgna1, a rice mutant generated from T-DNA insertion, has a short seminal root, adventitious roots and root hairs when grown at a low temperature (25°C).…”

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confidence: 99%

“…The length of lateral roots in 7-d-old seedlings of the osspr1 mutant is only 12% of that of the wild type. The OsSPR1 gene encodes a novel mitochondrial protein with an Armadillo-like repeat domain involved in iron homeostasis in rice [14].…”

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confidence: 99%

Fine-mapping of SRT7 for short roots and identification of its candidate in rice

et al. 2011

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Three allelic short root mutants were identified by screening mutants with defective root elongation of the rice japonica cultivar Nipponbare mutant library generated via 60 Co γ-ray irradiation mutagenesis. These mutants, designated srt7-1 (short root 7-1), srt7-2 and srt7-3, respectively, had an extremely short seminal root, adventitious roots and lateral roots. Histological observation revealed the cell length of srt7 mutant roots was significantly shorter than that of wild-type roots. Genetic analysis indicated the short root phenotype was controlled by a single recessive nuclear gene. The SRT7 gene was mapped to a 20-kb interval between the markers STS6 and STS7 on chromosome 4 by a map-based cloning method. Sequencing of the six predicted genes in this region found that all of the three allelic mutants contained a 1-bp or 2-bp deletion in the same gene encoding a putative membrane-bound endo-1,4-β-glucanase. The SRT7 gene was expressed ubiquitously, with higher levels of transcript accumulation in roots at different developmental stages. However, no difference was found in the SRT7 transcription level between the mutant and wild type. Collectively, these results indicate the endo-1,4-β-glucanase encoding gene (LOC_Os04g41970) is likely the candidate for SRT7 that functions posttranscriptionally in rice root elongation.rice, short root, endo-1,4-β-glucanase, map-based cloning Citation:Liu H J, Zheng H K, Wang H, et al. Fine-mapping of SRT7 for short roots and identification of its candidate in rice.

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Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa

Cited by 37 publications

References 43 publications

OsABCB14 functions in auxin transport and iron homeostasis in rice (Oryza sativa L.)

OsABCB14 functions in auxin transport and iron homeostasis in rice (Oryza sativa L.)

OsSPL3, an SBP-Domain Protein, Regulates Crown Root Development in Rice

Fine-mapping of SRT7 for short roots and identification of its candidate in rice

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