Identification of a novel integral plasma membrane protein induced during adipocyte differentiation

Albrektsen, Tatjana; Richter, Henrijette E.; Clausen, Jes Thorn; Fleckner, Jan

doi:10.1042/bj3590393

Cited by 32 publications

(16 citation statements)

References 40 publications

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“…4). Although the precise biological function of APMAP is unknown at present, it is considered a marker of adipocyte differentiation that is mostly present in mature adipocytes [59]. However, this was found only in WAT, and not in BAT.…”

Section: Figmentioning

confidence: 84%

Gender Difference in Proteome of Brown Adipose Tissues between Male and Female Rats Exposed to a High Fat Diet

Choi

et al. 2011

Cell Physiol Biochem

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Background: Although the importance of gender as a key determinant in health and illness has been recognized for a long time, systematic studies of gender differences in medicine are still lacking. We hypothesized that interscapular brown adipocyte tissue (BAT), is not only a key tissue contributing to energy expenditure, but also regulates diet-induced thermogenesis, and may be an ideal target for studying gender differences in obesity development in response to a high fat diet (HFD). Methods: We therefore performed differential proteome analysis of BAT from lean and obese rats of both genders fed a HFD using 2-DE combined with MALDI-TOF-MS. Results: When exposed to a HFD, male rats gained more body weight with increased values of plasma biochemical parameters than did female rats. Among 595 matched spots, 48 differentially expressed identified spots showed significant gender differences, whereas 7 proteins showed no gender differences, but did show a HFD response. Conclusions: Proteomic investigations into gender-dimorphic protein modulation in BAT may provide conclusive results showing higher expression of numerous proteins involved in thermogenesis and fat oxidation as well as lower expression of proteins contributing to fat synthesis in female rats than in male rats.

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Section: Figmentioning

confidence: 84%

Gender Difference in Proteome of Brown Adipose Tissues between Male and Female Rats Exposed to a High Fat Diet

Choi

et al. 2011

Cell Physiol Biochem

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“…Although the precise biological function of APMAP is unknown at present, it is considered a marker of adipocyte differentiation since it is present mostly in mature adipocytes [54]. The higher protein abundances of APMAP in HFD female rats (Fig.…”

Section: Proteins Showing Gender Differences With Higher Abundances Imentioning

confidence: 94%

Gender-dependent Protein Expression in White Adipose Tissues of Lean and Obese Rats Fed a High Fat Diet

Mukherjee¹,

Choi²,

Choi³

et al. 2012

Cell Physiol Biochem

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Background: Proper understanding of molecular mechanisms underlying gender dimorphism in obesity for better nutritional recommendation is still in early stages. As white adipose tissues (WAT) is most important tissue in obesity metabolism, comparative proteomic analysis of all three WAT deposits at the same time to yield immensely important protein markers was the primary goal of this study. Methods: We performed differential expression analysis of protein profiles of three different WAT viz. subcutaneous, inguinal, and abdominal fat deposits of both genders in lean and obese rats fed a high fat diet (HFD) using a combination of 2-DE and MALDI-TOF-MS. Results: The proteomics analysis enabled us to detect 25, 29, and 46 proteins showing gender differences in three WAT deposits, respectively, to gain insight into cause of higher body weight gain in male in response to HFD. Conclusion: The gender dimorphism found in this proteomic study implies that female rats have a lower tendency to undergo metabolic syndrome manifestation, which is associated with lower reliance on lipid as an energy fuel, lower lipogenesis, as well as increased mitochondrial oxidative capacity. In conclusion, most of the candidate proteins identified herein by differential proteomics were previously unrecognized in gender dimorphism of adipose tissue.

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“…In order to separate cellular compartments, a method developed by other research groups (Simpson et al 1983, Albrektsen et al 2001) was applied. After two washes in PBS, the cell monolayer was scraped into ice-cold PBS and washed once in sucrose holding buffer (SHB; 20 mM Tris/HCl, pH 7 .…”

Section: Subcellular Fractionationmentioning

confidence: 99%

Expression of TAU in insulin-secreting cells and its interaction with the calcium-binding protein secretagogin

Maj¹,

Gärtner²,

İlhan³

et al. 2010

Journal of Endocrinology

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Tauopathies have been associated with Alzheimer's disease (AD), which frequently manifests together with diabetes mellitus type 2. Calcium-binding proteins such as the recently identified secretagogin (SCGN) might exert protective effects. As pancreatic b-cells and neurons share common electrophysiological properties, we investigated the appearance of TAU (listed as MAPT in the HUGO and MGI Databases) protein at the islets of Langerhans and b-cellderived cell lines which highly express the neuroendocrinespecific protein SCGN. Six predominant TAU isoforms could be identified by immunoblotting, which formed TAU deposits detectable by immunofluorescence and sarkosylinsoluble pellets. Using GST-SCGN pull-down assays, a calcium-dependent SCGN-TAU interaction was found. In this line, sucrose density gradient fractionation and differential ultracentrifugation studies of TAU and SCGN revealed co-appearance of both proteins. Co-localization of TAU and SCGN within insulinoma cells and islets of Langerhans mainly restricted to insulin-positive b-cells was demonstrated by confocal microscopy. Motivated by these findings, we looked if SCGN overexpression could exert protective function on Rin-5F cells, which showed differences in TAU levels. Testing the vulnerability of Rin-5F clones by MTT assay, we revealed that high TAU levels going along with highest TAU aggregates could not be antagonized by high levels of SCGN protein. Our findings demonstrated for the first time the association of TAU and the calcium-binding protein SCGN and support earlier results implicating that b-cells might represent an extra cerebral site of tauopathy.

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Identification of a novel integral plasma membrane protein induced during adipocyte differentiation

Cited by 32 publications

References 40 publications

Gender Difference in Proteome of Brown Adipose Tissues between Male and Female Rats Exposed to a High Fat Diet

Gender Difference in Proteome of Brown Adipose Tissues between Male and Female Rats Exposed to a High Fat Diet

Gender-dependent Protein Expression in White Adipose Tissues of Lean and Obese Rats Fed a High Fat Diet

Expression of TAU in insulin-secreting cells and its interaction with the calcium-binding protein secretagogin

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