Identification of a novel herpesvirus associated with cutaneous ulcers in a fisher (<i>Martes pennanti</i>)

Gagnon, Carl A.; Tremblay, Josée; Larochelle, Danielle; Music, Nedzad; Tremblay, Donald

doi:10.1177/1040638711418615

Cited by 19 publications

(18 citation statements)

References 9 publications

(18 reference statements)

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“…These include GHV sequences identified in host species within the order Carnivora, including Darwin's fox (40), sea otter (41), oriental small-clawed otter (GenBank accession no. FJ797657), and fisher (42). Thus, it is likely that FcaGHV1 and LruGHV1 are part of a closely related subgroup of percaviruses of carnivores.…”

Section: Resultsmentioning

confidence: 99%

Novel Gammaherpesviruses in North American Domestic Cats, Bobcats, and Pumas: Identification, Prevalence, and Risk Factors

Troyer

Beatty

Stutzman-Rodriguez

et al. 2014

J Virol

135

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Gammaherpesviruses (GHVs) are a diverse and rapidly expanding group of viruses associated with a variety of disease conditions in humans and animals. To identify felid GHVs, we screened domestic cat (Felis catus), bobcat (Lynx rufus), and puma (Puma concolor) blood cell DNA samples from California, Colorado, and Florida using a degenerate pan-GHV PCR. Additional pan-GHV and long-distance PCRs were used to sequence a contiguous 3.4-kb region of each putative virus species, including partial glycoprotein B and DNA polymerase genes. We identified three novel GHVs, each present predominantly in one felid species: Felis catus GHV 1 (FcaGHV1) in domestic cats, Lynx rufus GHV 1 (LruGHV1) in bobcats, and Puma concolor GHV 1 (PcoGHV1) in pumas. To estimate infection prevalence, we developed real-time quantitative PCR assays for each virus and screened additional DNA samples from all three species (n ‫؍‬ 282). FcaGHV1 was detected in 16% of domestic cats across all study sites. LruGHV1 was detected in 47% of bobcats and 13% of pumas across all study sites, suggesting relatively common interspecific transmission. PcoGHV1 was detected in 6% of pumas, all from a specific region of Southern California. The risk of infection for each host varied with geographic location. Age was a positive risk factor for bobcat LruGHV1 infection, and age and being male were risk factors for domestic cat FcaGHV1 infection. Further characterization of these viruses may have significant health implications for domestic cats and may aid studies of free-ranging felid ecology. IMPORTANCEGammaherpesviruses (GHVs) establish lifelong infection in many animal species and can cause cancer and other diseases in humans and animals. In this study, we identified the DNA sequences of three GHVs present in the blood of domestic cats (Felis catus), bobcats (Lynx rufus), and pumas (Puma concolor; also known as mountain lions, cougars, and panthers). We found that these viruses were closely related to, but distinct from, other known GHVs of animals and represent the first GHVs identified to be native to these feline species. We developed techniques to rapidly and specifically detect the DNA of these viruses in feline blood and found that the domestic cat and bobcat viruses were widespread across the United States. In contrast, puma virus was found only in a specific region of Southern California. Surprisingly, the bobcat virus was also detected in some pumas, suggesting relatively common virus transmission between these species. Adult domestic cats and bobcats were at greater risk for infection than juveniles. Male domestic cats were at greater risk for infection than females. This study identifies three new viruses that are widespread in three feline species, indicates risk factors for infection that may relate to the route of infection, and demonstrates cross-species transmission between bobcats and pumas. These newly identified viruses may have important effects on feline health and ecology.

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Section: Resultsmentioning

confidence: 99%

Novel Gammaherpesviruses in North American Domestic Cats, Bobcats, and Pumas: Identification, Prevalence, and Risk Factors

Troyer

Beatty

Stutzman-Rodriguez

et al. 2014

J Virol

135

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“…There are many GHVs for which the gB-Dpol region has not been completely sequenced, and typically only a small fragment (160-220 bp) of the Dpol is available. Of these, GHVs found in sea otter (Enhydra lutris kenyoni; Tseng et al 2012), fisher (Martes pennanti; Gagnon et al 2011), oriental small-clawed otter (Aonyx cinerea; GenBank accession no. FJ797657), and Darwin's fox (Lycalopex fulvipes; Cabello et al 2013) would likely also cluster with MusHV1 and the felid percaviruses, based on high nucleotide homology of the short Dpol region (75-84%).…”

mentioning

confidence: 99%

“…Notably among the percaviruses, EHV2 and EHV5 have been linked to equine respiratory disease and poor performance (Fortier et al 2010). Among the carnivoreassociated percaviruses, fisher herpesvirus and mustelid herpesvirus 2 were isolated from animals with ulcerative lesions (Gagnon et al 2011;Tseng et al 2012), and FcaGHV1 was associated with poor health condition and increased incidence of FIV infection in cats . Thus, LpaGHV1 and LruGHV2 might play a role in the health of their felid hosts, although this remains to be determined.…”

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confidence: 99%

Identification of Novel Gammaherpesviruses in Ocelots (Leopardus pardalis) and Bobcats (Lynx rufus) in Panama and Colorado, USA

Lozano

Sweanor

Wilson-Henjum

et al. 2015

Journal of Wildlife Diseases

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ABSTRACT:Gammaherpesviruses (GHVs) have been identified in many species and are often associated with disease. Recently, we characterized three novel felid GHVs in domestic cats (Felis catus), bobcats (Lynx rufus), and pumas (Puma concolor). We investigated whether freeranging ocelots (Leopardus pardalis) and bobcats are infected with additional GHVs. We screened DNA samples from ocelots on Barro Colorado Island, Panama, and bobcats in western Colorado, US, by using a degenerate nested PCR that targets the GHV glycoprotein B gene. We identified a novel GHV glycoprotein B sequence in two ocelots and a second novel sequence in a bobcat, which is distinct from the previously characterized bobcat GHV (Lynx rufus GHV 1). Utilizing additional degenerate and virus-specific PCRs, we extended these sequences to include 3.4 kilobases of the GHV glycoprotein B and DNA polymerase genes. These sequences identify the first GHV detected in ocelots and the second GHV in bobcats. These viruses were provisionally named L. pardalis GHV 1 and Lynx rufus GHV 2, respectively. The viruses are most closely related to recently identified GHVs of the Percavirus genus found in domestic cats (F. catus GHV 1) and bobcats (L. rufus GHV 1), suggesting that a cluster of felid GHVs exists within the Percavirus genus.

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“…No papillomavirus DNA was obtained from the beluga whale lesion tissue. However, herpesvirus DNA was detected using a pan-herpesvirus nested PCR (nPCR), as described by Gagnon et al (2011). The nPCR targeted the herpesviruses DNA polymerase (DPOL) gene and detects a broad range of herpesvirus species.…”

mentioning

confidence: 99%

Identification of a Novel Herpesvirus Associated with a Penile Proliferative Lesion in a Beluga (Delphinapterus leucas)

Bellehumeur

Lair

Romero

et al. 2015

Journal of Wildlife Diseases

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The carcass of an adult male beluga (Delphinapterus leucas) was found beach cast in 2008 on the shore of the St. Lawrence Estuary at Riviè re-Ouelle, Quebec, Canada. The carcass was transported to the Faculté de mé decine vé té rinaire of the Université de Montré al for postmortem examination. Aspiration pneumonia was the probable cause of death. Necropsy revealed a focal papilloma-like penile lesion, characterized by focal mucosal thickening with disorganization of the epithelial layers and lymphoplasmacytic infiltration. A pan-herpesvirus nested PCR assay on frozen tissue from the penile lesion was positive. The PCR product sequencing revealed a partial herpesvirus DNA polymerase (DPOL) gene sequence of 600 nucleotides. Its nearest nucleotide identity was with the partial DPOL gene of an alphaherpesvirus, bovine herpesvirus 5 (79.5% identity). It also shared high identity with several other marine mammal herpesviruses (50.2 to 77.3% identity). This new herpesvirus was tentatively named beluga whale herpesvirus (BWHV). Virus isolation was unsuccessful. The pathogenic potential of BWHV is unknown, but the evaluation of archived tissues suggests that the virus is endemic in the St. Lawrence Estuary beluga population.

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Identification of a novel herpesvirus associated with cutaneous ulcers in a fisher (Martes pennanti)

Cited by 19 publications

References 9 publications

Novel Gammaherpesviruses in North American Domestic Cats, Bobcats, and Pumas: Identification, Prevalence, and Risk Factors

Novel Gammaherpesviruses in North American Domestic Cats, Bobcats, and Pumas: Identification, Prevalence, and Risk Factors

Identification of Novel Gammaherpesviruses in Ocelots (Leopardus pardalis) and Bobcats (Lynx rufus) in Panama and Colorado, USA

Identification of a Novel Herpesvirus Associated with a Penile Proliferative Lesion in a Beluga (Delphinapterus leucas)

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