Identification of a novel group of evolutionarily conserved members within the rapidly diverging murine Cea family

Carcinoembryonic antigen-related cell adhesion molecule 16 interacts with α-tectorin and is mutated in autosomal dominant hearing loss (DFNA4)

“…1C and Fig. S1C), suggesting that it is a secreted protein, a result consistent with an earlier prediction for CEACAM16 (24). In addition, subtilisin degraded CEACAM16 bands (Fig.…”

“…1A). Bioinformatic analysis also indicates that CEACAM16 is a secreted protein because it has a signal peptide at its N terminus with a proteolytic cleavage site between the 22nd and 23rd amino acids (24). To test this prediction, full-length Ceacam16 (∼1,300 bp) cloned from the cochlea was inserted into a mammalian expression vector with a V5-His epitope downstream of Ceacam16.…”

“…We also investigated whether α-tectorin can interact with other CEACAM proteins by performing coimmunoprecipitation between α-tectorin and CEACAM1, which is the most widely expressed CEACAM protein (24). OK cells were cotransfected with plasmids encoding α-tectorin and CEACAM1.…”

“…1A). Of the 22 Ceacam genes found in mice, Ceacam16 is one of only five conserved among mice, rats, and humans (24). In humans CEACAM16 is located on 19q13.31, where DFNA4, an autosomal dominant nonsyndromic hearing loss (ADNSHL) locus was mapped in an American family (Family 1070) (25).…”

Increased Glucose-induced Secretion of Glucagon-like Peptide-1 in Mice Lacking the Carcinoembryonic Antigen-related Cell Adhesion Molecule 2 (CEACAM2)

“…CEACAM1 and CEACAM2 are highly homologous proteins with differential tissue and cellular distribution (4,29) that predict distinct functions. CEACAM1 enhances insulin action by promoting insulin clearance in liver without affecting insulin secretion despite its significant expression in pancreatic ␤-cells (9).…”