Identification of a novel fungus, Trichoderma asperellum GDFS1009, and comprehensive evaluation of its biocontrol efficacy

Abstract

Due to its efficient broad-spectrum antimicrobial activity, Trichoderma has been established as an internationally recognized biocontrol fungus. In this study, we found and identified a novel strain of Trichoderma asperellum, named GDFS1009. The mycelium of T. asperellum GDFS1009 exhibits a high growth rate, high sporulation capacity, and strong inhibitory effects against pathogens that cause cucumber fusarium wilt and corn stalk rot. T. asperellum GDFS1009 secretes chitinase, glucanase, and protease, which can degrade the cell walls of fungi and contribute to mycoparasitism. The secreted xylanases are good candidates for inducing plant resistance and enhancing plant immunity against pathogens. RNA sequencing (RNA-seq) and gas chromatography-mass spectrometry (GC-MS) showed that T. asperellum GDFS1009 produces primary metabolites that are precursors of antimicrobial compounds; it also produces a variety of antimicrobial secondary metabolites, including polyketides and alkanes. In addition, this study speculated the presence of six antimicrobial peptides via ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF-MS/MS). Future studies should focus on these antimicrobial metabolites for facilitating widespread application in the field of agricultural bio-control.

Applications of Fungal Strains with Keratin-Degrading and Plant Growth Promoting Characteristics

“…Out of them, isolates showed strong antagonistic activity inhibiting over 50% mycelia growth of F. oxysporum, R.s olani and B. cinerea. Wu et al (2017) [41] reported an inhibition rate of 80.82% of new Trichoderma asperellum isolate against F. oxysporum, an aggressive pathogen infecting cucumber plants.…”

“…The secreted enzymes act synergistic each other, but at the same time with other metabolites. It has been reported that characteristics of chitinases are different at Trichoderma species [41].…”

Internal transcribed spacers (ITS) based identification of Trichoderma isolates and biocontrol activity against Macrophomina phaseolina, Aspergillus niger and Meloidogyne incognita

“…The effect of Trichoderma isolates on tomato plant growth parameters. J2), number of galls, egg masses and females per root system with 90.33, 90.59, 90.06 and 85.50%, respectively and the effect of this isolate was similar to vydate Wu et al (2017). isolated new strain of T. asperellum with high antagonism against pathogens of cucumber wilt and corn stalk rot.…”

“…These results confirm the efficiency of barcode DNA and are compatible with previous studies that employed ITS to identify Trichoderma spp. (Wu et al, 2017;Fahmi et al, 2016;Savitha and Sriram, 2015;Oskiera et al, 2015).…”

NOVEL SOLID-PHASE MULTIBIORECYCLED BIOLOGICS BASED ON Bacillus subtilis AND Trichoderma asperellum AS EFFECTIVE POTATO PROTECTANTS AGAINST Phytophthora DISEASE

“…Отмеченное в нашем исследовании увеличение биометрических показателей растений картофеля и рост урожайности, а также повышение эффек-тивности традиционной интегрированной защиты картофеля открывают перспективу разработки биопрепаратов защитного назначения, сочетающих в себе также свойства биоудобрений и биостимуляторов (39)(40)(41)(42)(43). Применение экспериментальных образцов полифункциональных биопрепаратов на основе T. asperellum Т-36 и B. subtilis B-10 в полевых условиях в принятых нормах расхода обеспечило достоверный защитный эффект, соответствующий описанному в мировых разработках у аналогичных препаратов (44)(45)(46)(47).…”