Identification of a novel cyclomaltodextrinase annotated as a neopullulanase in the genome of Bacillus cereus

Isolation, characterization, and cloning of thermostable pullulanase from Geobacillus stearothermophilus ADM-11

“…The purity of pullulanase was compared to 12 % by SDS-PAGE showed the presence of a single band 83 kDa. The purified pullulanase from different organisms showed great variation in molecular weight, such as purified pullulanase of 65 kDa from T. caldophilus GK-24 ( Ling et al, 2009 ), 68.25 kDa from B. cereus ( Park et al, 2023 ), and 76 kDa for Bacillus stearothermophilus ( Kuriki et al, 1990 ).…”

“…However, thermophilic bacteria can only thrive in specific environments. Many mesophiles and thermophiles have had their pullulanase genes successfully cloned and characterized ( Park et al, 2023 ).…”