“…The purity of pullulanase was compared to 12 % by SDS-PAGE showed the presence of a single band 83 kDa. The purified pullulanase from different organisms showed great variation in molecular weight, such as purified pullulanase of 65 kDa from T. caldophilus GK-24 ( Ling et al, 2009 ), 68.25 kDa from B. cereus ( Park et al, 2023 ), and 76 kDa for Bacillus stearothermophilus ( Kuriki et al, 1990 ).…”