Identification of a novel circularized transcript of the AML1 gene

Xu, Aining; Chen, Xiuhua; Tan, Yanhong; Qi, Xiling; Xu, Zhifang; Zhang, Lin-Lin; Ren, Fanggang; Bian, Sicheng; Chen, Yi; Wang, Hongwei

doi:10.5483/bmbrep.2013.46.3.171

Cited by 5 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Following the observation of circular molecules in viral genome, the first circRNAs in eukaryotic cells were confirmed in the cytoplasmic region of HeLa cells [4], and yeast mitochondrial RNA [5]. Subsequent evidence on individual circRNAs was then continually reported in different human and rat tissues, for example circRNAs derived from ETS-1, SRY, P450, ABP, DYSTROPHIN, MBL, and AML [6][7][8][9][10][11][12]. Though multiple experiments prove the existence of circRNAs, the identified circRNAs were generally less abundant than the linear products from the same parental genes.…”

Section: Background: Origin Of Circrnas As Functional Noncoding Rnasmentioning

confidence: 99%

Functional role of circular RNAs in cancer development and progression

2018

View full text Add to dashboard Cite

Circular RNAs (circRNAs) are a large class of endogenously expressed non-coding RNAs formed by covalently closed loops through back-splicing. High throughput sequencing technologies have identified thousands of circRNAs with high sequence conservation and cell type specific expression in eukaryotes. CircRNAs play multiple important roles in cellular physiology functioning as miRNA sponges, transcriptional regulators, RBP binding molecules, templates for protein translation, and immune regulators. In a clinical context, circRNAs expression is correlated with patient's clinicopathological features in cancers including breast, liver, gastric, colorectal, and lung cancer. Additionally, distinct properties of circRNAs, such as high stability, exonuclease resistance, and existence in body fluids, show promising role for circRNAs as molecular biomarkers for tumor diagnosis, non-invasive monitoring, prognosis, and therapeutic intervention. Therefore, it is critical to further understand the molecular mechanism underlying circRNAs interaction in tumors and the recent progress of this RNA species in cancer development. In this review, we provide a detailed description of biological functions, molecular role of circRNAs in different cancers, and its potential role as biomarkers in a clinical context.

show abstract

Section: Background: Origin Of Circrnas As Functional Noncoding Rnasmentioning

confidence: 99%

Functional role of circular RNAs in cancer development and progression

2018

View full text Add to dashboard Cite

show abstract

“…To evaluate cytokine mRNA expression levels, RAW264.7 cells pre-treated with MPP (0~50 µM) for 30 min were incubated with LPS (1 µg/ml) for 6 h. Total RNA was isolated using TRIzol Reagent (Gibco BRL) according to the manufacturer's instructions, and stored at –70℃ until further use. Semiquantitative RT reactions were conducted as previously reported [ 19 ]. The primers (Bioneer, Seoul, Korea) used in these experiments are listed in Table 1 (F: forward, R: reverse).…”

Section: Methodsmentioning

confidence: 99%

(E)-3-(3-methoxyphenyl)-1-(2-pyrrolyl)-2-propenone displays suppression of inflammatory responses via inhibition of Src, Syk, and NF-κB

Kim

Jeong

Lee

et al. 2016

Korean J Physiol Pharmacol

View full text Add to dashboard Cite

(E)-3-(3-methoxyphenyl)-1-(2-pyrrolyl)-2-propenone (MPP) is an aldol condensation product resulting from pyrrole-2-carbaldehyde and m- and p- substituted acetophenones. However, its biological activity has not yet been evaluated. Since it has been reported that some propenone-type compounds display anti-inflammatory activity, we investigated whether MPP could negatively modulate inflammatory responses. To do this, we employed lipopolysaccharide (LPS)-stimulated macrophage-like RAW264.7 cells and examined the inhibitory levels of nitric oxide (NO) production and transcriptional activation, as well as the target proteins involved in the inflammatory signaling cascade. Interestingly, MPP was found to reduce the production of NO in LPS-treated RAW264.7 cells, without causing cytotoxicity. Moreover, this compound suppressed the mRNA levels of inflammatory genes, such as inducible NO synthase (iNOS) and tumor necrosis factor (TNF)-α. Using luciferase reporter gene assays performed in HEK293 cells and immunoblotting analysis with nuclear protein fractions, we determined that MPP reduced the transcriptional activation of nuclear factor (NF)-κB. Furthermore, the activation of a series of upstream signals for NF-κB activation, composed of Src, Syk, Akt, and IκBα, were also blocked by this compound. It was confirmed that MPP was able to suppress autophosphorylation of overexpressed Src and Syk in HEK293 cells. Therefore, these results suggest that MPP can function as an anti-inflammatory drug with NF-κB inhibitory properties via the suppression of Src and Syk.

show abstract

“…To evaluate cytokine mRNA expression levels, RAW264.7 cells pre-treated with Gl-ME (0-300 μg/mL) for 30 min were incubated with LPS (1 μg/mL) for 6 h. Total RNA was isolated using TRIzol Reagent (Gibco BRL) according to the manufacturer's instructions and stored at À70°C until further use. Semiquantitative RT reactions were conducted as previously reported (Xu et al, 2013). The primers (Bioneer, Seoul, Korea) used in these experiments are listed in Table 1 (F: forward, R: reverse).…”

Section: Methodsmentioning

confidence: 99%

Anti-inflammatory Activities of Gouania leptostachya Methanol Extract and its Constituent Resveratrol

et al. 2014

View full text Add to dashboard Cite

Gouania leptostachya DC. var. tonkinensis Pitard. Rhamnaceae is a traditional medicinal plant used in Thailand for treating various inflammatory symptoms. However, no systematic studies have been performed concerning the anti-inflammatory effects or molecular mechanisms of this plant. The immunopharmacological activities of a methanol extract from the leaves and twigs of G. leptostachya (Gl-ME) were elucidated based on the gastritis symptoms of mice treated with HCl/EtOH and the inflammatory responses, such as nitric oxide (NO) release and prostaglandin E2 (PGE2) production, from RAW264.7 cells and peritoneal macrophages. Moreover, inhibitory target molecules were also assessed. Gl-ME dose-dependently diminished the secretion of NO and PGE2 from LPS-stimulated RAW264.7 cells and peritoneal macrophages. The gastritis lesions of HCl/EtOH-treated mice were also attenuated after Gl-ME treatment. The extract (50 and 300 µg/mL) clearly reduced mRNA expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2, nuclear translocation of p65/nuclear factor (NF)-κB, phosphorylation of p65-activating upstream enzymes, such as protein kinase B (AKT), inhibitor of κBα kinase (IKK), and inhibitor of κB (IκBα), and the enzymatic activity of Src. By HPLC analysis, one of the major components in the extract was revealed as resveratrol with NO and Src inhibitory activities. Moreover, this compound suppressed NO production and HCl/EtOH-induced gastric symptoms. Therefore, these results suggest that Gl-ME might be useful as an herbal anti-inflammatory medicine through the inhibition of Src and NF-κB activation pathways. The efficacy data of G. leptostachya also implies that this plant could be further tested to see whether it can be developed as potential anti-inflammatory preparation.

show abstract

Identification of a novel circularized transcript of the AML1 gene

Cited by 5 publications

References 22 publications

Functional role of circular RNAs in cancer development and progression

Functional role of circular RNAs in cancer development and progression

(E)-3-(3-methoxyphenyl)-1-(2-pyrrolyl)-2-propenone displays suppression of inflammatory responses via inhibition of Src, Syk, and NF-κB

Anti-inflammatory Activities of Gouania leptostachya Methanol Extract and its Constituent Resveratrol

Contact Info

Product

Resources

About