Identification of a Novel 2S Albumin with Antitryptic Activity from Caryocar brasiliense Seeds

Abstract

Proteinaceous and non-proteinaceous compounds with insecticidal activities have been isolated from plant sources. Here, protein extracts from Pequi seeds (Caryocar brasiliense) showed in vitro activity against trypsin-like enzymes. However, no activity was obtained against cancer cells, phytopathogenic fungi or human pathogenic bacteria. Further purification was performed by using trypsin-Sepharose affinity chromatography. Electrophoretic analysis revealed a single protein compound with molecular mass of approximately 14 kDa. Under reducing conditions, two peptides with smaller molecular masses were observed in SDS-PAGE, showing the presence of a heterodimeric peptide. MALDI-ToF/ToF sequencing was performed with a protein band removed from SDS-PAGE, and the sequence revealed high identity with the 2S albumin family. Purified peptide was challenged in vitro against bovine trypsin and Spodoptera frugiperda digestive enzymes by using BApNA as a substrate, showing high inhibitory activity in both cases. Data here reported suggest that a 2S albumin dimeric peptide could show biotechnological potential for S. frugiperda control. In summary, Cb2SA could be useful in controlling insect pests of agricultural interest.

Control of Meloidogyne incognita Using Aqueous Extracts and Solanum stramonifolium Jacq. Dialisates

“…On the other hand, studies using extract, fractions, and isolated metabolites from C. ensiformis indicated their activity against M. incognita but did not present hemolytic activity (Rocha et al, 2017). Studies using protein extract of Caryocar brasiliense seeds demonstrated no hemolytic activity, despite its insecticidal potential against pest insects in in vitro tests (Costa, Franco, Migliolo, & Dias, 2015). Solanum capsicoides, a plant from the Solanaceae family, also showed no hemolytic effect (Petreanu et al, 2016).…”

Purification and Characterization of 2S Albumin from Seeds of Wrightia tinctoria Exhibiting Antibacterial and DNase Activity

“…The 2S albumins generally have conserved set of cysteine residues which forms four disulfide bridges; two inter-chain bridges found between smaller and larger subunit; and other two intra-chain bridges present within the larger subunit [30,31]. Apart from this, 2S albumins performs plethora of functions such as antibacterial, antifungal and antitryptic and serine protease inhibitory activity as reported from different plant species [32][33][34][35]. The exact mechanism of the antifungal and antibacterial activity of 2S albumins is not well characterized.…”