Identification of a New, Unorthodox Member of the MAGE Gene Family

Põld, Mehis; Zhang, Jin; Chen, Grace L.; Hall, Jeffrey M.; Vescio, Robert; Berenson, James R.

doi:10.1006/geno.1999.5870

Cited by 72 publications

(43 citation statements)

References 14 publications

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“…Although the expression of the other CTSP-1 family members was also restricted among normal tissues, they were not frequently expressed in tumor cell lines and tumor samples. This fact has also been observed for other CT-antigen members of the MAGE and SSX families (17)(18)(19). A positive expression in normal testis was detected for CTSP-2 and -4, the first also being expressed in brain, fetal brain, and spinal cord among the 21 normal tissues analyzed (data not shown).…”

supporting

confidence: 71%

Characterization of a cancer/testis (CT) antigen gene family capable of eliciting humoral response in cancer patients

Parmigiani

Bettoni

Vibranovski

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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supporting

confidence: 71%

Characterization of a cancer/testis (CT) antigen gene family capable of eliciting humoral response in cancer patients

Parmigiani

Bettoni

Vibranovski

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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“…14 A cancer-testis antigen MAGE-E1 was identified using SAGE (serial analysis of gene expression) technique. 33 MAGE-D1, 34 SAGE and HAGE 35 were identified using RDA (representational difference analysis) method. Our study is the first report for identification of a novel melanoma antigen with melanocyte preferential expression using DNA chip and serum immunodetection.…”

Section: Discussionmentioning

confidence: 99%

Novel melanoma antigen, FCRL/FREB, identified by cDNA profile comparison using DNA chip are immunogenic in multiple melanoma patients

Inozume

Matsuzaki

Kurihara

et al. 2004

Intl Journal of Cancer

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“…3A. A putative human counterpart of mouse Dlxin-1 has been reported as MAGE-D1 (18). In the data base, SNERG-1 (GenBank TM accession number AF274043) from rat testis showed a high homology (96%) to mouse Dlxin-1, suggesting that SNERG-1 is a rat orthologue of mouse Dlxin-1 (Fig.…”

Section: Isolation Of a Molecule That Binds To The N-terminal Domain mentioning

confidence: 95%

Dlxin-1, a Novel Protein That Binds Dlx5 and Regulates Its Transcriptional Function

Masuda¹,

Sasaki²,

Shibuya³

et al. 2001

Journal of Biological Chemistry

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Dlx5, a member of the Dlx family of homeodomain proteins, plays a critical role in bone development and fracture healing. To understand the molecular mechanism underlying the transcriptional regulation by Dlx5, we performed yeast two-hybrid screening and isolated a novel protein, Dlxin-1, that binds Dlx5 and regulates its transcriptional function. Dlxin-1 cDNA encodes a 775-amino acid protein that has a partial homology with necdin at the C terminus and 25 repeats of hexapeptides (WQXPXX) in the middle region. Dlxin-1 mRNA is expressed in various adult tissues, but not the spleen, and also in osteoblastic and chondrogenic cell lines. During embryogenesis, a strong signal for Dlxin-1 mRNA was found in cell layers surrounding cartilaginous elements in bone rudiment during digit formation. Dlxin-1 binds not only Dlx5 but also Dlx7 and Msx2 and forms homomultimers in vivo. Transfection and reporter gene assays indicate that Dlxin-1 activates the transcriptional function of Dlx5. Therefore, Dlxin-1 may act as a regulator of the function of Dlx family members in bone formation.The Dlx gene family, which comprises at least six mammalian homologues of the Drosophila homeodomain protein Distal-less, is expressed predominantly in forebrain, limbs, and branchial arches during fetal development (1, 2). Among this family, Dlx5 is expressed in most developing skeletal elements and is induced during the process of bone fracture healing (2-6), suggesting that it may play a crucial role in bone development and formation. Msx2 is expressed in skeletal elements and many other organs (7-9). The expression of Dlx5 mRNA, as well as Msx2, is induced by bone morphogenetic protein (5, 7, 10). It has been suggested that Dlx5 is a transcriptional activator and regulates osteoblastic functions positively (4,5,11,12). In contrast, Ryoo et al. (13) have demonstrated, using a reporter gene assay, that Dlx5 is a potential negative regulator of the expression of the rat osteocalcin gene, which is specifically expressed in bone. Recently, Acampora et al. (14) and Depew et al. (15) have reported the phenotype of mice homozygous for targeted deletion of the dlx5 gene, in which the expression of the osteocalcin gene in osteoblasts was markedly increased, suggesting that Dlx5 represses the expression of the osteocalcin gene in vivo (14).To understand the molecular mechanism(s) underlying the transcriptional regulation involving Dlx5, we attempted to identify a molecule that binds Dlx5 and regulates its function. Here we report the isolation and characterization of Dlxin-1, a novel protein that binds Dlx5 and regulates its transcriptional function. Dlxin-1 is a unique member of the necdin/melanomaassociated antigen (MAGE) 1 family (16 -18). MATERIALS AND METHODS Cells-KUSA/A1(19), a mouse osteoblastic cell line, and HT1080, a human fibrosarcoma cell line, were kindly provided by Drs. A. Umezawa (Keio University, Japan) and A. Fukamizu (University of Tsukuba, Japan), respectively. A yeast strain, Y153, was a gift from Dr. S. Kato (University of Tok...

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Identification of a New, Unorthodox Member of the MAGE Gene Family

Cited by 72 publications

References 14 publications

Characterization of a cancer/testis (CT) antigen gene family capable of eliciting humoral response in cancer patients

Characterization of a cancer/testis (CT) antigen gene family capable of eliciting humoral response in cancer patients

Novel melanoma antigen, FCRL/FREB, identified by cDNA profile comparison using DNA chip are immunogenic in multiple melanoma patients

Dlxin-1, a Novel Protein That Binds Dlx5 and Regulates Its Transcriptional Function

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