Identification of a new type of resistance breaking strain of tomato spotted wilt virus on tomato bearing the Sw-5b resistance gene

Almási, Asztéria; Pinczés, Dóra; Tímár, Zoltán; Sáray, Réka; Palotás, Gábor; Salánki, Katalin

doi:10.1007/s10658-023-02656-5

Cited by 7 publications

(4 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TSWV strains overcoming resistance against Sw-5b in tomato was reported from various regions at different time points [95,96]. The resistance-breaking phenotype was associated with amino acid substitutions in NSm, namely C118Y, T120N, and D122G [97][98][99]. Similarly, in pepper, TSWV-resistant breaking strains were reported from different regions [100,101].…”

Section: Sw-5b and Tsw Genesmentioning

confidence: 99%

Deciphering the Role of Virus Receptors in Plant–Virus–Vector Interactions

Jangra,

Chinnaiah,

Patil

et al. 2024

Receptors

View full text Add to dashboard Cite

Insect-transmitted plant viruses are a major threat to global agricultural crop production. Receptors play a prominent role in the interplay between host-pathogen and vector interaction. The virus–vector relationship involves both viral and vector receptors. Receptors-like kinases (RLKs) and receptor-like proteins play a crucial role in plant immunity, which acts as a basal defense. Pathogens can evade or block host recognition by their effector proteins to inhibit pathogen recognition receptor (PRR)-mediated signaling. Intriguingly, RLKs are also known to interact with viral proteins and impact plant susceptibility against viruses, while the endocytic receptors in vectors assist in the binding of the virus to the vectors. Unlike other receptors of fungi and bacteria which have three different domains located from extracellular or intracellular to perceive a multitude of molecular patterns, the characterization of viral receptors is quite complex and limited since the virus is directly injected into plant cells by insect vectors. Little is known about these receptors. Unraveling the receptors involved in virus entry and transmission within the vector will provide vital information in virus–vector interactions. This review focuses on efforts undertaken in the identification and characterization of receptors of plant viruses within the host and vector. This will lead to a better understanding of the cellular mechanism of virus transmission and spread, and further suggests new alternative tools for researchers to develop an integrated approach for the management of viral diseases and associated vectors.

show abstract

Section: Sw-5b and Tsw Genesmentioning

confidence: 99%

Deciphering the Role of Virus Receptors in Plant–Virus–Vector Interactions

Jangra,

Chinnaiah,

Patil

et al. 2024

Receptors

View full text Add to dashboard Cite

show abstract

“…For crop resistance, Sw-5b- and Tsw -mediated single gene resistance was deployed in commercial cultivars of tomato and pepper, respectively ( Boiteux and de Ávila, 1994 ; Boiteux, 1995 ; Dianese et al., 2011 ; de Oliveira et al., 2018 ). However, such a resistance has exerted tremendous selection pressure on TSWV, which led to the emergence of resistance breaking (RB) strains worldwide ( Aramburu and Martí, 2003 ; Margaria et al., 2004 ; Ciuffo et al., 2005 ; Sharman and Persley, 2006 ; Margaria et al., 2007 ; Zaccardelli et al., 2008 ; Fidan and Sari, 2019 ; Yoon et al., 2021 ; Almási et al., 2023 ). In the US, tomato-infecting RB strains capable of infecting an array of commercial tomato cultivars have been reported in California, North Carolina, and most recently in Texas by the Gadhave lab ( Batuman et al., 2016 ; Chinnaiah et al., 2023b ; Lahre et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Using Raman spectroscopy for early detection of resistance-breaking strains of tomato spotted wilt orthotospovirus in tomatoes

Juárez,

Steczkowski,

Chinnaiah

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

Tomato spotted wilt (TSW) disease caused by tomato spotted wilt orthotospovirus (TSWV, Orthotospovirus tomatomaculae) poses a significant threat to specialty and staple crops worldwide by causing over a billion dollars in crop losses annually. Current strategies for TSWV diagnosis heavily rely on nucleic acid or protein-based techniques which require significant technical expertise, and are invasive, time-consuming, and expensive, thereby catalyzing the search for better alternatives. In this study, we explored the potential of Raman spectroscopy (RS) in early detection of TSW in a non-invasive and non-destructive manner. Specifically, we investigated whether RS could be used to detect strain specific TSW symptoms associated with four TSWV strains infecting three differentially resistant tomato cultivars. In the acquired spectra, we observed notable reductions in the intensity of vibrational peaks associated with carotenoids. Using high-performance liquid chromatography (HPLC), we confirmed that TSWV caused a substantial decrease in the concentration of lutein that was detected by RS. Finally, we demonstrated that Partial Least Squares-Discriminant Analysis (PLS-DA) could be used to differentiate strain-specific TSW symptoms across all tested cultivars. These results demonstrate that RS can be a promising solution for early diagnosis of TSW, enabling timely disease intervention and thereby mitigating crop losses inflicted by TSWV.

show abstract

“…Subsequently, other aa substitution mutations have been identified in this NSm region that also allow for RB, e.g., T120N, C118F, and D122G. The T120N and C118F strains have been exclusively reported from Spain and Mexico, respectively [ 18 , 22 ]; whereas the D122G strain was reported from Australia, Hungary and the US (North Carolina) [ 18 , 23 , 24 ]. Together, these results demonstrate TSWV is rapidly overcoming Sw-5b resistance, and spotted wilt could reach pandemic levels in areas where varieties with this resistance gene are exclusively grown.…”

Section: Introductionmentioning

confidence: 99%

An all-out assault on a dominant resistance gene: Local emergence, establishment, and spread of strains of tomato spotted wilt orthotospovirus (TSWV) that overcome Sw-5b-mediated resistance in fresh market and processing tomatoes in California

Macedo,

Melgarejo,

Cespedes

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Tomato spotted wilt orthotospovirus (TSWV) causes substantial economic loss to tomato production, and the Sw-5b resistance gene is widely deployed for management. Here, we show (i) the emergence of resistance-breaking (RB) TSWV strains in processing and fresh market tomato production in California over the past ten years, and (ii) evolutionary relationships with RB strains from other areas. A specific RT-PCR test was used to show the C118Y RB strain that emerged in Fresno County in 2016 quickly became predominant in the central production area and remained so through this study. In 2021, the C118Y strain was detected in the Northern production area, and was predominant in 2022. However, in 2023, the C118Y strain was unexpectedly detected in fewer spotted wilt samples from resistant varieties. This was due to emergence of the T120N RB strain, previously known to occur in Spain. A specific RT-PCR test was developed and used to show that the T120N RB strain was predominant in Colusa and Sutter counties (detected in 75–80% of samples), and detected in ~50% of samples from Yolo County. Pathogenicity tests confirmed California isolates of the T120N strain infected Sw-5b tomato varieties and induced severe symptoms. Another RB strain, C118F, was associated with spotted wilt samples of Sw-5 varieties from fresh market tomato production in southern California. Phylogenetic analyses with complete NSm sequences revealed that the C118Y and T120N RB strains infecting resistant processing tomato in California emerged locally, whereas those from fresh market production were more closely related to isolates from Mexico. Thus, widespread deployment of this single dominant resistance gene in California has driven the local emergence of multiple RB strains in different tomato production areas and types. These results further emphasize the need for ongoing monitoring for RB strains, and identification of sources of resistance to these strains.

show abstract

Identification of a new type of resistance breaking strain of tomato spotted wilt virus on tomato bearing the Sw-5b resistance gene

Cited by 7 publications

References 30 publications

Deciphering the Role of Virus Receptors in Plant–Virus–Vector Interactions

Deciphering the Role of Virus Receptors in Plant–Virus–Vector Interactions

Using Raman spectroscopy for early detection of resistance-breaking strains of tomato spotted wilt orthotospovirus in tomatoes

An all-out assault on a dominant resistance gene: Local emergence, establishment, and spread of strains of tomato spotted wilt orthotospovirus (TSWV) that overcome Sw-5b-mediated resistance in fresh market and processing tomatoes in California

Contact Info

Product

Resources

About