Identification of a New Type of Colicine (Colicine L)

Hauduroy, P; Papavassiliou, J.

doi:10.1038/195730a0

Cited by 8 publications

(2 citation statements)

References 3 publications

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“…To indicate colicin production, a combination of five strains was chosen, granting to detect production of any colicin type known so far or combinations thereof. These were: universal colicin indicators Escherichia coli K12-Row, C6 (ϕ ) (GRATIA 1925) and B1 (ŠMARDA 1960), indicating all known colicin types and combinations thereof, and two indicators for specific colicin types: Escherichia coli P400, indicating colicin L (HAUDUROY and PAPAVASSILIOU 1962) and Shigella sonnei 17, indicating colicin Js (ABBOTT and GRAHAM 1961). No colicin typification could be performed in this study, due to a high number of colicinogenic strains revealed.…”

Section: Methodsmentioning

confidence: 99%

Incidence of colicinogenic strains among humanEscherichia coli

Šmarda

Obdržálek

2001

J. Basic Microbiol.

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During the years 1993–1999, altogether 1,043 Escherichia coli strains from colons of different persons were screened for colicinogeny using a most susceptible procedure and indicator system. In control persons (with healthy colons), 41.37% producers of colicins were found. In patients suffering from salmonelloses, the proportion of colicinogenic Escherichia coli was the same. In patients with non‐specific inflammatory colon diseases, the proportion of colicinogenic Escherichia coli strains appeared slightly though weakly, significantly or unsignificantly increased: to 47.50% in morbus Crohn and to 56.10% in colitis ulcerosa. These results suggest some sort of engagement of colicinogeny in the pathogenesis thereof. In malignant tumours of the colon, the incidence of colicinogenic Escherichia coli was not altered (40.58%). This does not indicate any colicin participation in the pathology of malignant tumours. In colitis ulcerosa, the incidence of colicinogenic Escherichia coli strains inhibiting Shigella sonei 17 (the indicator for colicin Js which generally inhibits interoinvasive strains of both species) increased from 21.94 % (control samples) to 41.46%. Uropathogenic Escherichia coli strains shared the same incidence of colicinogeny as controls (42.08%), if they were not haemolytic; haemolytic ones were colicinogenic with only 22.37%. This difference was highly significant. The patterns of some colicin activities in the set of five indicator strains used suggested that several wild strains produced new, so far unknown types of colicins and/or combinations thereof.

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Section: Methodsmentioning

confidence: 99%

Incidence of colicinogenic strains among humanEscherichia coli

Šmarda

Obdržálek

2001

J. Basic Microbiol.

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“…Bacterial strains. The colicinogenic strains were described elsewhere (4, 8,11,17). All other strains were derivatives of E. coli K-12 and are listed in Bacteriophages.…”

Section: Methodsmentioning

confidence: 99%

Outer membrane of Escherichia coli K-12: differentiation of proteins 3A and 3B on acrylamide gels and further characterization of con (tolG) mutants

Manning¹,

Reeves²

1976

J Bacteriol

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Two classes of mutants, con and toiG, that appeared to be very similar in a number of respects have been shown to be identical and cotransducible with pyrD. By diethylaminoethyl-cellulose chromatography of the outer membranes, we have shown that the mutants are missing only protein 3A and retain protein 3B. Using con mutants, we were thus able to identify protein 3B on the pH 7.2 gel system of Maizel where it runs separately from protein 3A if unheated samples are used. tolG mutants were shown to be identical to con mutants in being conjugation defective with most F-like plasmid donors but not with I-like plasmid donors, and in their resistance pattern to bacteriophages and colicins.During the course ofthis study, it was observed that the bacteriocin produced by Serratia marcescens JF246 was identical in its activity spectrum to colicin L-398 and is now considered to be a colicin of type L.Recent studies of bacteriophage-and colicinresistant mutants ofEschericha coli have led to the identification of outer membrane proteins able to be used as receptors for these bacteriophages and colicins, and also to characterization of the biological role of the proteins.Sabet and Schnaitman (30) showed that bfe mutants, resistant to colicins El, E2, E3, and A and to bacteriophage BF23, have lost the receptor for at least some of these lethal agents and that this receptor is a protein involved in the transport of vitamin B12 (7). Bacteriophages Ti, T5, and 480 and colicin M also share a

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Colicins—Exocellular lethal proteins ofEscherichia coli

Šmarda

Šmajs

1998

Folia Microbiol

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Colicins are toxic exoproteins produced by bacteria of colicinogenic strains of Escherichia coli and some related species of Enterobacteriaceae, during the growth of their cultures. They inhibit sensitive bacteria of the same family. About 35% E. coli strains appearing in human intestinal tract are colicinogenic. Synthesis of colicins is coded by genes located on Col plasmids. Until now more than 34 types of colicins have been described, 21 of them in greater detail, viz. colicins A, B, D, E1-E9, Ia, Ib, JS, K, M, N, U, 5, 10. In general, their interaction with sensitive bacteria includes three steps: (1) binding of the colicin molecule to a specific receptor in the bacterial outer membrane; (2) its translocation through the cell envelope; and (3) its lethal interaction with the specific molecular target in the cell. The classification of colicins is based on differences in the molecular events of these three steps.

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Identification of a New Type of Colicine (Colicine L)

Cited by 8 publications

References 3 publications

Incidence of colicinogenic strains among humanEscherichia coli

Incidence of colicinogenic strains among humanEscherichia coli

Outer membrane of Escherichia coli K-12: differentiation of proteins 3A and 3B on acrylamide gels and further characterization of con (tolG) mutants

Colicins—Exocellular lethal proteins ofEscherichia coli

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