Identification of a new source of interference leached from polypropylene tubes in mass-selective analysis

Xia, Yuanqing; Patel, Shefali; Bakhtiar, Ray; Franklin, Ronald B.; Doss, George A.

doi:10.1016/j.jasms.2004.11.020

Cited by 18 publications

(14 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[2] A sulfoxide oxidative product along with a sulfone oxidative of didodecyl 3,3 -thiodipropionate (DDTDP) that is used to prevent oxidative degradation of synthetic polymers leaching from the polypropylene tubes has been reported and the chemicals were identified with mass spectroscopy. [3] Plasticizers outgasing from O-rings can lead to undesirable ion-molecule chemistry in an electrospray quadruple ion trap mass spectrometer. [4] Recently, it was reported that compounds such as di(2-hydroxyethyl)methyldodecylammonium (DiHEMDA) and 9-octadecenamide (oleamide) leaching from polypropylene tubes hamper the measurements in DNA and protein assays.…”

Section: Introductionmentioning

confidence: 99%

Quick detection of contaminants leaching from polypropylene centrifuge tubes with surface‐enhanced Raman spectroscopy and ultraviolet absorption spectroscopy

Gartia

Choi

et al. 2011

J Raman Spectroscopy

View full text Add to dashboard Cite

Anomalous surface-enhanced Raman scattering (SERS) peaks were identified for liquid sample stored in polypropylene (PP) centrifuge tubes for months. We observed unexpected Raman peaks during experiments with thiamine hydrochloride aqueous solutions stored in PP tubes for 2 months. In order to identify the contaminants, we have performed SERS experiments on deionized (DI) water stored in PP centrifuge tubes for 2 months and compared them with those from fresh DI water sample. We have also carried out ultraviolet (UV) absorption spectra for both fresh and contaminated water. We believe that the water is contaminated because of chemicals leaching from the PP tube. From the gas chromatography-mass spectrometry data, the main contaminants were found to be phthalic acid (PA) and its derivatives. Further SERS and UV absorption experiment for PA correlated well with the anomalous peaks identified earlier. We qualitatively confirmed the identification and quantitatively estimated the concentration of the suspect contaminants as between 1 and 10 µM with both SERS and UV absorption spectroscopy. With UV absorption spectroscopy, we precisely estimated the concentration as 2.1 µM. We have shown that the sample in PP tube can be contaminated by the leaching chemicals upon long-term storage, and suggest SERS and UV absorption spectroscopy as two quick and simple techniques to detect the contamination.

show abstract

Section: Introductionmentioning

confidence: 99%

Quick detection of contaminants leaching from polypropylene centrifuge tubes with surface‐enhanced Raman spectroscopy and ultraviolet absorption spectroscopy

Gartia

Choi

et al. 2011

J Raman Spectroscopy

View full text Add to dashboard Cite

show abstract

“…Chemical noise therefore imposes a fundamental limit on the detection of trace analytes [10]. Many obscure sources of contamination in mass spectrometry have been identified [9,[11][12][13][14], while still others remain to be exposed [18].…”

mentioning

confidence: 99%

Cyclic polyamide oligomers extracted from nylon 66 membrane filter disks as a source of contamination in liquid chromatography/mass spectrometry

Tran

Doucette

2006

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Background interferences are perhaps an unavoidable part of analytical detection schemes, particularly when analyzing trace level samples or when using detectors with high levels of sensitivity. In liquid chromatography, mobile phase solvents are routinely filtered using membrane filter disks to trap particulates in hopes of minimizing contamination, providing improvements in data output and instrumental operation. In this study, we report that one such filter disk leads to a significant level of contamination in LC and LC/MS experiments. Extractable compounds from nylon membrane filters generate significant background signals in UV absorption chromatograms at 214 nm, and are also detected by electrospray ionization mass spectrometry, with nominal m/z values of 453 and 679. It is shown that rinsing the nylon membranes before their use can reduce, but will not eliminate, the extractable contaminants from the mobile phase. Through MS and tandem MS analysis, we have identified these contaminants as cyclic oligomers of polyamide 66. Based on these results, it is recommended that nylon membrane filters be avoided when conducting trace level analysis, particularly when conducting LC/MS experiments. (J Am Soc Mass Spectrom 2006, 17, 652-656)

show abstract

“…The (M + 2Na – H) + and (M + Na + K – H) + , and (2M + K) + ions for this species are also detected, as are (M + H) + and (2M + Na) + and (2M + K) + ions for a species oxidized to the sulfoxide derivative. Dilauryl thiodipropanoate is a common antioxidant found in plasticizers and cosmetics 37 and has been detected as a leachate from polypropylene tubes 38 but is not known to be endogenous to soybeans; therefore, these peaks were excluded in the analysis of metabolite changes according to growing season detailed below. Thus, while ethanol may effectively extract many components from soybean leaves, it is important for future studies to recognize potential contamination from dilauryl thiodipropionate onto leaf surfaces if specimens are stored in plastic bags used in food packaging; it may be advisible to use glass Petri dishes instead.…”

Section: Resultsmentioning

confidence: 99%

High-Throughput Metabolic Profiling of Soybean Leaves by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

et al. 2015

View full text Add to dashboard Cite

As a relatively recent research field, plant metabolomics has gained increasing interest in the past few years and has been applied to answer biological questions through large-scale qualitative and quantitative analyses of the plant metabolome. The combination of sensitivity and selectivity offered by mass spectrometry (MS) for measurement of many metabolites in a single shot makes it an indispensable platform in metabolomics. In this regard, Fourier-transform ion cyclotron resonance (FTICR) has the unique advantage of delivering high mass resolving power and mass accuracy simultaneously, making it ideal for the study of complex mixtures such as plant extracts. Here we optimize soybean leaf extraction methods compatible with high-throughput reproducible MS-based metabolomics. In addition, matrix-assisted laser desorption ionization (MALDI) and direct LDI of soybean leaves are compared for metabolite profiling. The extraction method combined with electrospray (ESI)-FTICR is supported by the significant reduction of chlorophyll and its related metabolites as the growing season moves from midsummer to the autumn harvest day. To our knowledge for the first time, the use of ESI-FTICR MS and MALDI-FTICR MS is described in a complementary manner with the aim of metabolic profiling of plant leaves that have been collected at different time points during the growing season.

show abstract

Identification of a new source of interference leached from polypropylene tubes in mass-selective analysis

Cited by 18 publications

References 6 publications

Quick detection of contaminants leaching from polypropylene centrifuge tubes with surface‐enhanced Raman spectroscopy and ultraviolet absorption spectroscopy

Quick detection of contaminants leaching from polypropylene centrifuge tubes with surface‐enhanced Raman spectroscopy and ultraviolet absorption spectroscopy

Cyclic polyamide oligomers extracted from nylon 66 membrane filter disks as a source of contamination in liquid chromatography/mass spectrometry

High-Throughput Metabolic Profiling of Soybean Leaves by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Contact Info

Product

Resources

About