Identification of a new Sarcocystis sp. in marsh deer (Blastocerus dichotomus) from wetlands of Argentina

Berra, Yanina; Moré, Gastón; Helman, Elisa; Argibay, Hernán Darío; Orozco, María Marcela

doi:10.1016/j.ijppaw.2022.12.007

Cited by 5 publications

(2 citation statements)

References 24 publications

(61 reference statements)

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“…from small mammals to their definitive hosts, rather than to their intermediate hosts, have been shown in a series of studies [ 61 , 85 ]. Currently, possible definitive hosts of Sarcocystis species are suggested based on phylogenetic results [ 86 , 87 , 88 , 89 ]. The phylogenetic analysis of this work showed that the presumed definitive hosts of S .…”

Section: Discussionmentioning

confidence: 99%

Protozoan Parasites of Sarcocystis spp. in Rodents from Commercial Orchards

Prakas,

Stirkė,

Šneideris

et al. 2023

Animals

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Small mammals are an important group of wildlife that can transmit pathogens to humans and animals. There is a lack of comprehensive studies on the protozoan parasites of the genus Sarcocystis in agricultural areas. The aim of the current research was to evaluate the prevalence of Sarcocystis spp., and to identify the parasite species found in the skeletal muscles of rodents and insectivores from commercial orchards. A total of 679 muscle samples from small mammals, mainly rodents (n = 674), belonging to eight species were examined. Muscle samples were pooled into groups, then digested, and the presence of the Sarcocystis species was confirmed by molecular methods. The examined parasites were determined in five rodent species, Apodemus agrarius, A. flavicollis, Clethrionomys glareolus, Microtus arvalis, and M. oeconomus. The prevalence of Sarcocystis spp. was low: 2.23% in voles and 0.79% in mice. Based on a sequence comparison of cox1 and 28S rDNA, four species were identified: S. myodes, Sarcocystis cf. strixi, Sarcocystis sp. Rod1, and Sarcocystis sp. Rod2. This is the first report of S. myodes in A. agrarius, A. flavicollis, and M. arvalis. The identified species were most closely related to Sarcocystis spp., and were transmitted by predatory mammals and birds. Future studies are needed to describe the species morphologically, as well as to define the host spectrum and to evaluate their possible pathogenicity.

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Section: Discussionmentioning

confidence: 99%

Protozoan Parasites of Sarcocystis spp. in Rodents from Commercial Orchards

Prakas,

Stirkė,

Šneideris

et al. 2023

Animals

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“…When performing molecular identi cation, it is important to select an appropriate genetic region for the separation of the studied Sarcocystis species [20]. Based on available knowledge, cox1 (cytochrome c oxidase subunit 1) gene is the most suitable for identifying Sarcocystis species employing ungulates as their intermediate hosts [21][22][23]. Since sporocyst concentrations are often low in de nitive hosts, nested PCR is frequently used for the ampli cation of the target genetic region [16,17].…”

Section: Introductionmentioning

confidence: 99%

The Distribution of Sarcocsytis Species Described by Ungulates-Canids Life Cycle in Intestines of Small Predators of the Family Mustelidae

Šneideris,

Moskaliova,

Butkauskas

et al. 2024

Acta Parasit.

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PurposeUsing molecular techniques, we have previously shown that carnivorous mammals of the family Mustelidae might be common de nitive hosts for various protozoan Sarcocystis species. In the present study we aimed to unravel whether Sarcocystisspecies using ungulates as intermediate hosts and canids or felids as de nitive hosts can be found in intestine of mustelids. MethodsSmall intestine samples of 93 individual mustelids of ve different species from Lithuania were examined. Sarcocystis species were identi ed based on species-speci c PCR and subsequent cox1 sequencing. ResultsSix Sarcocystis species (S. arieticanis, S. bertrami, S. capracanis, S. capreolicanis, S. linearis and S. morae) de ned by ungulate-canid life cycle were detected for the rst time in small intestines of mustelids. By contrast, the prevalence of Sarcocystis characterised by ungulate-felid life cycle was low (3.2 %). Overall, 76 % of the examined animals were positive for at least one of the studied Sarcocystis species. Four species, S. arieticanis, S. bertrami, S. capracanis and S. moraewere most commonly found, with the detection rate of about 40 %. ConclusionsCurrent nding, in addition to our previous studies, suggests that mustelids play an important role in the spread of various Sarcocystisspecies.

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Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina

Bentancourt Rossoli,

Moré,

Soto-Cabrera

et al. 2023

Parasitol Res

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The occurrence of Sarcocystis species was investigated in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina. Nine species were captured (n = 356). Sarcocysts were detected in muscles of 8.7% (31/356) and 3.7% (4/106) of the rodents by histopathology and direct microscopy, respectively. PCRs-sequencing targeting the 18S rRNA, cox1 and ITS1 regions were performed on samples with positive histopathology. Four different 18S rRNA sequences or sequence groups with high intra-group identities (99.6-100%) were detected in Mus musculus, Oxymycterus rufus, Akodon azarae and Necromys lasiurus. Eight sequences showed 99.5-99.7% identity with S. dispersa. Thirteen sequences showed low identity (95.3-96.4%) with other Sarcocystis spp. The obtained coxI sequences (n = 9) were almost identical among them and showed a high similarity with S. strixi (99.2-99.5%) and S. lutrae (99.1%), despite the 18S rRNA sequences from the same samples suggested the occurrence of at least two species. This suggests that coxI may not show high variability in Sarcocystis spp. that use rodents as intermediate hosts. Six ITS1 sequences were obtained, showing high identity but low coverage with several Sarcocystis spp. Multilocus sequence typing and BLAST analysis did not allow accurate species identi cation. Possible reasons are the detection of new species or the lack of molecular information from previously described ones. Phylogeny suggests that the detected Sarcocystis spp. may use raptor birds or snakes as de nitive hosts. This study represents the rst molecular identi cation of Sarcocystis spp. in naturally infected rodents of the Cricetidae and Muridae families in South America.

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Identification of a new Sarcocystis sp. in marsh deer (Blastocerus dichotomus) from wetlands of Argentina

Cited by 5 publications

References 24 publications

Protozoan Parasites of Sarcocystis spp. in Rodents from Commercial Orchards

Protozoan Parasites of Sarcocystis spp. in Rodents from Commercial Orchards

The Distribution of Sarcocsytis Species Described by Ungulates-Canids Life Cycle in Intestines of Small Predators of the Family Mustelidae

Identification of Sarcocystis spp. in synanthropic (Muridae) and wild (Cricetidae) rodents from Argentina

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