Identification of a new Neisseria meningitidis serogroup C clone from Anhui province, China

Shao, Zhujun; Li, Wei; Ren, Jun; Liang, Xiaofeng; Xu, Li; Diao, Baowei; Li, Machao; Lu, Meijuan; Ren, Hao; Cui, Zhigang; Zhu, Bingqing; Dai, Zhen-Wei; Zhang, Li; Chen, Xia; Kan, Biao; Xu, Jianguo

doi:10.1016/s0140-6736(06)68141-5

Cited by 89 publications

(92 citation statements)

References 16 publications

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“…The approach we developed can be applied to many bacterial species. For example, in Neisseria meningitidis, a subpopulation referred to as the ST4821 complex causes about 300 human infections annually in China (48,49), and a genome sequencing-based scheme would be highly beneficial. Our core genome sequence typing system provides a timely implementation of strain identification and classification using high-throughput NGS genome data, facilitating the application of genome data to the fields of clinical medicine, epidemiology, and surveillance.…”

Section: Discussionmentioning

confidence: 99%

Minimum Core Genome Sequence Typing of Bacterial Pathogens: a Unified Approach for Clinical and Public Health Microbiology

et al. 2013

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Bacterial pathogens impose a heavy health burden worldwide. In the new era of high-throughput sequencing and online bioinformatics, real-time genome typing of infecting agents, and in particular those with potential severe clinical outcomes, holds promise for guiding clinical care to limit the detrimental effects of infections and to prevent potential local or global outbreaks. Here, we sequenced and compared 85 isolates of Streptococcus suis, a zoonotic human and swine pathogen, wherein we analyzed 32 recognized serotypes and 75 sequence types representing the diversity of the species and the human clinical isolates with high public health significance. We found that 1,077 of the 2,469 genes are shared by all isolates. Excluding 201 common but mobile genes, 876 genes were defined as the minimum core genome (MCG) of the species. Of 190,894 single-nucleotide polymorphisms (SNPs) identified, 58,501 were located in the MCG genes and were referred to as MCG SNPs. A population structure analysis of these MCG SNPs classified the 85 isolates into seven MCG groups, of which MCG group 1 includes all isolates from human infections and outbreaks. Our MCG typing system for S. suis provided a clear separation of groups containing human-associated isolates from those containing animal-associated isolates. It also separated the group containing outbreak isolates, including those causing life-threatening streptococcal toxic shock-like syndrome, from sporadic or less severe meningitis or bacteremia-only isolates. The typing system facilitates the application of genome data to the fields of clinical medicine and epidemiology and to the surveillance of S. suis. The MCG groups may also be used as the taxonomical units of S. suis to define bacterial subpopulations with the potential to cause severe clinical infections and large-scale outbreaks.

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Section: Discussionmentioning

confidence: 99%

Minimum Core Genome Sequence Typing of Bacterial Pathogens: a Unified Approach for Clinical and Public Health Microbiology

et al. 2013

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“…Pulsed-field gel electrophoresis (PFGE) was performed according to the method described by Shao et al (12). Briefly, chromosomal DNA was digested with NheI and separated on 1.0% SeaKem gold agarose in 0.5ϫ Trisborate-EDTA buffer using the CHEF-DR III system from Bio-Rad (Hercules, CA) with the following parameters: initial pulse, 1 s; final pulse, 25 s with linear ramping; voltage, 6 V/cm; time of electrophoresis, 16 h; and temperature, 14°C.…”

Section: Methodsmentioning

confidence: 99%

Genetic Study of Capsular Switching between Neisseria meningitidis Sequence Type 7 Serogroup A and C Strains

et al. 2010

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Neisseria meningitidis is a leading cause of septicemia and meningitis worldwide. N. meningitidis capsular polysaccharides have been classified into 13 distinct serogroups which are defined by antibody reactivity and structural analysis, and the capsule plays an important role in virulence. Serogroups A, B, C, W135, and Y have been reported to be clinically important. Several newly identified serogroup C isolates belonging to the unique sequence type 7 (ST-7) were identified in China. Since most ST-7 isolates from China belonged to serogroup A, the newly identified ST-7 serogroup C strains were proposed to have arisen from those belonging to ST-7 serogroup A. In this study, six ST-7 serogroup C and three ST-7 serogroup A isolates were analyzed by pulsed-field gel electrophoresis to confirm their sequence type. In order to clarify the genetic basis of capsular switching between ST-7 serogroup A and C strains, the whole capsular gene clusters and surrounding genes of the two representative ST-7 strains belonging to serogroups A and C, respectively, were sequenced and compared. Potential recombination sites were analyzed using the RDP3 beta software, and recombinationrelated regions in two other ST-7 serogroup A and five ST-7 serogroup C strains were also sequenced and compared to the representative ST-7 serogroup A and C strain sequences.

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“…In some bacteria, diseases were caused by a specific clone complex, which spread and caused an outbreak. Those genotypes are favoured by selection and expand, thus creating an 'epidemic' population structure (Shao et al, 2006). The heterogeneous characteristics of the NTHi strains may be the reason that no outbreak or epidemics occurred among children of Chengdu city.…”

Section: J Microbiolmentioning

confidence: 99%

Genotypic characteristics of Haemophilus influenzae isolates from pediatric pneumonia patients in Chengdu city, Sichuan, China

Tian

Zhang

et al. 2009

J Microbiol.

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Two hundred and seventy-three Haemophilus influenzae strains isolated from pediatric pneumonia patients in China were studied. We used Multilocus Sequence Typing (MLST) to analyze genotypic characteristics. All strains were biotyped and serotyped. Relatedness and patterns of genes among isolates were determined by the analysis of MLST and eBURST. H. influenzae primarily causes acute pneumonia in children under 1 year old. Nontypeable H. influenzae was responsible for most cases of pediatric pneumonia. All 273 strains were classified into eight biotypes. They mostly belonged to the I, II, and III biotypes (17.6%, 43.6%, and 22.7%, respectively). 62 strains (22.7%) produced beta-lactamase. We found 28 novel alleles. Fifty different STs were found by MLST, of which 39 were novel. These were ST477 through ST508 and ST521 through ST527. Group 17 and predicted founders 503 were new groups in this study. No STs correlated with strains from Korea, which is adjacent to China. The H. influenzae strains from China appeared to have heterogeneous ST types patterns which may be the reason no outbreaks or epidemics of H. influenzae infections have occurred in Chengdu city, Sichuan, China.

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Identification of a new Neisseria meningitidis serogroup C clone from Anhui province, China

Cited by 89 publications

References 16 publications

Minimum Core Genome Sequence Typing of Bacterial Pathogens: a Unified Approach for Clinical and Public Health Microbiology

Minimum Core Genome Sequence Typing of Bacterial Pathogens: a Unified Approach for Clinical and Public Health Microbiology

Genetic Study of Capsular Switching between Neisseria meningitidis Sequence Type 7 Serogroup A and C Strains

Genotypic characteristics of Haemophilus influenzae isolates from pediatric pneumonia patients in Chengdu city, Sichuan, China

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