Identification of a new molecular marker C2-25 linked to the Fusarium oxysporum f.sp. radicis-lycopersici resistance Frl gene in tomato

Staniaszek, Mirosława; Szczechura, Wojciech; Marczewski, Waldemar

doi:10.17221/70/2014-cjgpb

Cited by 5 publications

(5 citation statements)

References 10 publications

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“…9, as reported earlier (Vakalounakis et al 1997, Fazio et al 1999, Mutlu et al 2015, indicating that the FORL resistance in 'AV107-4' might have originated from the Frl of an S. peruvianum accession. However, the results on the genomic location of Frl were contradictory between recently published D r a f t studies and our study: by using an F 2 population, Staniaszek et al (2014) mapped the COSII marker C2_At2g38025 at 3 cM distal to Frl in line '3070'. The C2_At2g38025 was positioned at 45 cM of Chr.…”

Section: Discussioncontrasting

confidence: 99%

“…9 and in locations linked to FORL resistance (Mutlu et al 2015). However, similar to the findings of Staniaszek et al (2014), our present results strongly suggested that FORL resistance in 'AV107-4' was closely linked to the PNU-D4 marker, which is in the vicinity of 6.0 Mb (50.0 cM of Chr. 9 on Tomato-EXPEN 2000 map) for which genetic recombination (crossing over) is severely suppressed.…”

Section: Discussionsupporting

confidence: 86%

“…found in 'AV107-4'. Physical and genetic map locations of PNU-D4 and other previously reported FORLlinked markers(Staniaszek et al 2014) indicated that FORL resistance genes other than Frl might exist.Further, genomic rearrangement events in Chr. 9, such as inversion, might have resulted in the discrepancy in the chromosomal location of Frl in different FORL resistance tomato germplasm.…”

mentioning

confidence: 65%

“…By using an F 2 population, Staniaszek et al (2014) identified a tight linkage of 3 cM between Frl and a conserved ortholog set II (COSII) marker C2_At2g38025 that was positioned at 45 cM of Chr. 9 on Tomato-EXPEN 2000 map.…”

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confidence: 99%

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Development of a high-resolution melting marker for selecting Fusarium crown and root rot resistance in tomato

Kim

et al. 2016

Genome

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Fusarium crown and root rot is a severe fungal disease of tomato caused by Fusarium oxysporum f. sp. radicis-lycopersici (FORL). In this study, the genomic location of the FORL-resistance locus was determined using a set of molecular markers on chromosome 9 and an F2 population derived from FORL-resistant inbred 'AV107-4' (Solanum lycopersicum) × susceptible 'L3708' (Solanum pimpinellifolium). Bioassay performed using Korean FORL strain KACC 40031 showed single dominant inheritance of FORL resistance in the F2 population. In all, 13 polymerase chain reaction-based markers encompassing approximately 3.6-72.0 Mb of chromosome 9 were developed based on the Tomato-EXPEN 2000 map and SolCAP Tomato single nucleotide polymorphism array analysis. These markers were genotyped on 345 F2 plants, and the FORL-resistance locus was found to be present on a pericentromeric region of suppressed chromosomal recombination in chromosome 9. The location of the FORL-resistance locus was further confirmed by testing these markers against diverse commercial tomato and stock cultivars resistant to FORL. A restriction fragment length polymorphism marker, PNU-D4, located at approximately 6.1 Mb of chromosome 9 showed the highest match with the resistance locus and was used for conducting high-resolution melting analysis for marker-assisted selection of FORL resistance.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 86%

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confidence: 65%

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confidence: 99%

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Development of a high-resolution melting marker for selecting Fusarium crown and root rot resistance in tomato

Kim

et al. 2016

Genome

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“…We were unable to find any bacterial isolate that could control both the Fol R3 and Forl pathogens ( Table 1 ), thus rejecting our first hypothesis. To date, no resistance to Forl has been described in tomato ( Ates et al, 2019 ), and even though the Frl resistance gene that confers partial resistance to Forl was identified in 2001 ( Staniaszek et al, 2014 ), the nature of this gene is still unknown. It is possible that the antagonistic mechanisms required to inhibit growth of both pathogens could be very different, which would explain why we could not find a common isolate able to control both fungal tomato pathogens.…”

Section: Discussionmentioning

confidence: 99%

Rhizospheric bacteria as potential biocontrol agents against Fusarium wilt and crown and root rot diseases in tomato

Khalil

Fierro-Coronado

Peñuelas-Rubio

et al. 2021

Saudi Journal of Biological Sciences

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The discovery of novel biocontrol agents requires the continuous scrutiny of native microorganisms to ensure that they will be useful on a regional scale. The goal of the present work was to discover novel antagonistic bacteria against Fusarium oxysporum ff. spp. lycopersici race 3 ( Fol R3) and radicis-lycopersici ( Forl ) causing Fusarium wilt disease and Fusarium crown and root rot of tomatoes, respectively. High-throughput liquid antagonism screening of 1,875 rhizospheric bacterial strains followed by dual confrontation assays in 96-well plates was used to select bacteria exhibiting > 50% fungal growth inhibition. In a second dual confrontation assay in 10-cm Petri dishes, bacteria showing > 20% Fol R3 or Forl growth inhibition were further screened using a blood hemolysis test. After discarding β-hemolytic bacteria, a seedling antagonistic assay was performed to select five potential antagonists. A phylogenetic analysis of 16S rRNA identified one strain as Acinetobacter calcoaceticus ( Ac DB3) and four strains as members of the genus Bacillus ( B. amyloliquefaciens Ba MA26, Bacillus siamensis Bsi DA2, B. subtilis Bs TA16 and B. thuringiensis Bt MB9). Greenhouse assays demonstrated that Bs TA16 and Ac DB3 were the most promising antagonists against Fol R3 and Forl , respectively. Pathogen biocontrol and growth promotion mechanisms used by these bacteria include the production of siderophores, biofilm, proteases, endoglucanases and indole acetic acid, and phosphate solubilization. These five bacteria exerted differential responses on pathogen control depending on the tomato hybrid, and on the growth stage of tomatoes. We report for the first time the use of an Acinetobacter calcoaceticus isolate ( Ac DB3) to control Forl in tomato under greenhouse conditions.

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Development of co-dominant SCAR markers linked to resistant gene against the Fusarium oxysporum f. sp. radicis-lycopersici

Mutlu

Demirelli²,

İlbi

et al. 2015

Theor Appl Genet

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We developed highly reliable co-dominant SCAR markers linked to the Frl gene. FORL testing is difficult. The marker is expected to be quickly adapted for MAS by tomato breeders. Fusarium oxysporum f. sp. radicis-lycopersici causes Fusarium crown and root rot (FCR), an economically important soil-borne disease of tomato. The resistance against FCR is conferred by a single dominant gene (Frl) located on chromosome 9. The aim of this study was to develop molecular markers linked to the Frl gene for use in marker-assisted breeding (MAS) programs. The FCR-resistant 'Fla. 7781' and susceptible 'B560' lines were crossed, and F1 was both selfed and backcrossed to 'B560' to generate segregating F2 and BC1 populations. The two conserved set II (COSII) markers were found linked to the Frl gene, one co-segregated with FCR resistance in both F2 and BC1 populations and the other was 8.5 cM away. Both COSII markers were converted into co-dominant SCAR markers. SCARFrl marker produced a 950 and a 1000 bp fragments for resistant and susceptible alleles, respectively. The linkage of SCARFrl marker was confirmed in BC2F3 populations developed by backcrossing the resistant 'Fla. 7781' to five different susceptible lines. The SCARFrl marker has been in use in the tomato breeding programs in BATEM, Antalya, Turkey, since 2012 and has proved highly reliable. The SCARFrl marker is expected to aid in the development of FCR-resistant lines via marker-assisted selection (MAS).

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Identification of a new molecular marker C2-25 linked to the Fusarium oxysporum f.sp. radicis-lycopersici resistance Frl gene in tomato

Cited by 5 publications

References 10 publications

Development of a high-resolution melting marker for selecting Fusarium crown and root rot resistance in tomato

Development of a high-resolution melting marker for selecting Fusarium crown and root rot resistance in tomato

Rhizospheric bacteria as potential biocontrol agents against Fusarium wilt and crown and root rot diseases in tomato

Development of co-dominant SCAR markers linked to resistant gene against the Fusarium oxysporum f. sp. radicis-lycopersici

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