Identification of a new HLA‐DRB 1*13 allele (DRB 1*1326) with a short DRB1*16 sequence

Voorter, Christina E.M.; Bruyn-Geraets, D. De; Verduyn, W.; Schreuder, G. M. T.; Berg‐Loonen, Ella M. van den

doi:10.1111/j.1399-0039.1997.tb02718.x

Cited by 10 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For forward and reverse sequencing, a group specific 5′ primer, DRB95003 was used together with the generic DRB95008. The internal Cy‐5 conjugated DR95035c and DRB96005c primers were used for the forward and reverse sequencing (4). To obtain the 5′ end of the new allele, the generic 5′ primer DRB96027 was used in combination with the allele specific antisense primer 259 (5) and the Cy‐5 conjugated DRB96031c.…”

Section: Drb1 Primers Used For Sequencing Sequence and Locationmentioning

confidence: 99%

Identification of a novel HLA DRB1 exon 2 sequence, DRB1*1345

et al. 2002

View full text Add to dashboard Cite

Five different haplotypes in the human HLA D region are recognized based on their gene composition. The HLA DR52 associated DRB1 alleles include DRB1*03/08/11/12/13/14 and are characterized by the YST sequence motif in the codons 10-12 but vary in other polymorphic regions. The mechanisms generating the extensive variability are not entirely clear. Some alleles have probably arisen from point mutations, but most polymorphism has probably been caused by intralocus gene conversion, and the distinction between the ancient serologically defined groups is more and more difficult. This report describes the identification of a novel DRB1* allele - DRB1*1345 - found in a kidney transplant recipient from East Africa. The new allele shows the closest resemblance to DRB1*1114 and DRB1*1323. It differs from both those alleles at codons 57, 58 and 60, where the new allele carries the 'A-H' sequence motif common to DRB1*14 alleles such as 1401/04/07/10/16/25. This motif is only found in one other DRB1*13 allele namely DRB1*1343.

show abstract

Section: Drb1 Primers Used For Sequencing Sequence and Locationmentioning

confidence: 99%

Identification of a novel HLA DRB1 exon 2 sequence, DRB1*1345

et al. 2002

View full text Add to dashboard Cite

show abstract

“…Genomic DNA was isolated using QIA-AMP kits following the suppliers protocol (Qiagen, Westburg, Leusden, NL). Sequence-based typing was performed for the class I1 genes DRB1, DRB3/4/5, DQB1 and DPBl using a solid-phase sequencing approach as described before (3,4). An ALF express DNA sequencer was used for sequencing and subtype assignment was performed with the Sequityper software (Pharmacia).…”

mentioning

confidence: 99%