Identification of a new function of metallothionein-like gene OsMT1e for cadmium detoxification and potential phytoremediation

Rono, Justice Kipkorir; Wang, Le Le; Wu, Xue; Cao, Hong; Zhao, Ye; Khan, Irfan Ullah; Yang, Zhi Min

doi:10.1016/j.chemosphere.2020.129136

Cited by 65 publications

(36 citation statements)

References 59 publications

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“…It is produced in response to heavy metal ions entering the plant [ 124 , 125 ]. The study found that the Cd tolerance and accumulation ability of yeast enhanced after inserting rice MT1e [ 126 ], which was consistent with the results of Gu et al [ 127 ], and Chen et al [ 128 ]. The binding of heavy metal ions to the cysteine-rich regions may be one of the mechanisms that plants use for detoxification [ 129 ].…”

Section: The Mechanisms Of Plant Enrichment Of CDsupporting

confidence: 86%

Research on the Mechanisms of Plant Enrichment and Detoxification of Cadmium

Yang

Zheng

Tan

et al. 2021

Biology

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The heavy metal cadmium (Cd), as one of the major environmentally toxic pollutants, has serious impacts on the growth, development, and physiological functions of plants and animals, leading to deterioration of environmental quality and threats to human health. Research on how plants absorb and transport Cd, as well as its enrichment and detoxification mechanisms, is of great significance to the development of phytoremediation technologies for ecological and environmental management. This article summarises the research progress on the enrichment of heavy metal cadmium in plants in recent years, including the uptake, transport, and accumulation of Cd in plants. The role of plant roots, compartmentalisation, chelation, antioxidation, stress, and osmotic adjustment in the process of plant Cd enrichment are discussed. Finally, problems are proposed to provide a more comprehensive theoretical basis for the further application of phytoremediation technology in the field of heavy metal pollution.

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Section: The Mechanisms Of Plant Enrichment Of CDsupporting

confidence: 86%

Research on the Mechanisms of Plant Enrichment and Detoxification of Cadmium

Yang

Zheng

Tan

et al. 2021

Biology

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“…as well as MTs (Zimeri et al, 2005;Pagani et al, 2012;Rono et al, 2021). In contrast, U. compressa cultivated with 10 µM copper showed an increase in PC2 and PC4 on days 5 to 12, and no decrease in their levels compared with cadmium, and this increase was concomitant with enhanced expression of UcMT occurring on days 5 to 12 (Navarrete et al, 2019).…”

Section: Cadmium-induced Synthesis Of Phytochelatins and Increased Expression Of Metallothioneinmentioning

confidence: 92%

“…In addition, cadmium can be sequestered by small (around 10 kDa) cysteine-rich proteins named metallothioneins (MTs) that coordinate divalent metal ions via sulfhydryl groups of cysteines ( Cobbett and Goldsbrough, 2002 ). It has been shown that plant MTs are involved in the tolerance and accumulation of cadmium and other heavy metals in plants ( Zimeri et al, 2005 ; Pagani et al, 2012 ; Rono et al, 2021 ). In addition, oxidative stress induced by cadmium activates MAPK signal transduction pathway that involves MAPKKK that phosphorylates MAPKK that, in turn, phosphorylates MAPK that activate transcription factors and gene expression ( Gallego et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Cadmium Accumulation Involves Synthesis of Glutathione and Phytochelatins, and Activation of CDPK, CaMK, CBLPK, and MAPK Signaling Pathways in Ulva compressa

2021

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In order to analyze the effect of cadmium in Ulva compressa (Chlorophyta), the alga was cultivated with 10, 25, and 50 μM of cadmium for 7 days, and the level of intracellular cadmium was determined. Intracellular cadmium showed an increase on day 1, no change until day 5, and an increase on day 7. Then, the alga was cultivated with 10 μM for 7 days, and the level of hydrogen peroxide, superoxide anions, and lipoperoxides; activities of antioxidant enzymes ascorbate peroxidase (AP), dehydroascorbate reductase (DHAR), and glutathione reductase (GR); the level of glutathione (GSH) and ascorbate (ASC); and the level of phytochelatins (PCs) and transcripts encoding metallothioneins (UcMTs) levels were determined. The level of hydrogen peroxide increased at 2 and 12 h, superoxide anions on day 1, and lipoperoxides on days 3 to 5. The activities of AP and GR were increased, but not the DHAR activity. The level of GSH increased on day 1, decreased on day 3, and increased again on day 5, whereas ASC slightly increased on days 3 and 7, and activities of enzymes involved in GSH and ASC synthesis were increased on days 3 to 7. The level of PC2 and PC4 decreased on day 3 but increased again on day 5. The level of transcripts encoding UcMT1 and UcMT2 increased on days 3 to 5, mainly that of UcMT2. Thus, cadmium accumulation induced an oxidative stress condition that was mitigated by the activation of antioxidant enzymes and synthesis of GSH and ASC. Then, the alga cultivated with inhibitors of calcium-dependent protein kinases (CDPKs), calmodulin-dependent protein kinases (CaMKs), calcineurin B-like protein kinases (CBLPKs), and MAPKs and 10 μM of cadmium for 5 days showed a decrease in intracellular cadmium and in the level of GSH and PCs, with the four inhibitors, and in the level of transcripts encoding UcMTs, with two inhibitors. Thus, CDPKs, CaMK, CBLPKS, and MAPKs are involved in cadmium accumulation and GSH and PC synthesis, and GSH and PCs and/or UcMTs may participate in cadmium accumulation.

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“…It points to a bond between a metal ion and thionein (a cysteine rich apoprotein) rather than describing a function of these kinds of proteins per se , as is the case, for example, with the ADHs discovered a couple of years earlier [ 6 ]. Though it was not immediately obvious what MTs can and cannot do, and what purpose they might serve in a living organism, MTs became the focus of an intense and often controversial corpus of research since the 1960s (e.g., [ 7 , 8 , 9 , 10 , 11 , 12 ]). From a biochemical perspective, there is no question that MTs have unique properties.…”

Section: Introductionmentioning

confidence: 99%

The Enigmatic Metallothioneins: A Case of Upward-Looking Research

Abdin

Jacob

Kästner

2021

IJMS

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In the mid-1950s, Bert Lester Vallee and his colleague Marvin Margoshes discovered a molecule referred to today as metallothionein (MT). Meanwhile, MTs have been shown to be common in many biological organisms. Despite their prevalence, however, it remains unclear to date what exactly MTs do and how they contribute to the biological function of an organism or organ. We investigate why biochemical research has not yet been able to pinpoint the function(s) of MTs. We shall systematically examine both the discovery of and recent research on Dr. Vallee’s beloved family of MT proteins utilizing tools from philosophy of science. Our analysis highlights that Vallee’s initial work exhibited features prototypical of a developing research tradition: it was upward-looking, exploratory, and utilized mere interactions. Since the 1960s, MT research has increasingly become intervention- and hypothesis-based while it remained largely upward-looking in character. Whilst there is no reason to think that upward-looking research cannot successfully yield structure-function mappings, it has not yet been successful in the case of MTs. Thus, we suggest it might be time to change track and consider other research strategies looking into the evolution of MTs. Recent studies in mollusks render research in this direction worthy of pursuit.

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Identification of a new function of metallothionein-like gene OsMT1e for cadmium detoxification and potential phytoremediation

Cited by 65 publications

References 59 publications

Research on the Mechanisms of Plant Enrichment and Detoxification of Cadmium

Research on the Mechanisms of Plant Enrichment and Detoxification of Cadmium

Cadmium Accumulation Involves Synthesis of Glutathione and Phytochelatins, and Activation of CDPK, CaMK, CBLPK, and MAPK Signaling Pathways in Ulva compressa

The Enigmatic Metallothioneins: A Case of Upward-Looking Research

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