Identification of a new Bacillus sonorensis strain KLBC GS-3 as a biocontrol agent for postharvest green mould in grapefruit

Deng, Jia; Kong, Shanshan; Wang, Fang; Liu, Yun; Jiao, Jun-Ying; Lu, Yao; Zhang, Fan; Wu, Jianrong; Wang, Lianchun; Li, Xianzhong

doi:10.1016/j.biocontrol.2020.104393

Cited by 23 publications

(11 citation statements)

References 57 publications

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“…The same phenomenon occurs in VOCs produced by bacteria, the B. cinerea mycelium treated with Bacillus amyloliquefaciens BA17 showed swelling and dehydration deformity, protoplast aggregation, mitochondria enlargement and increase, and cell wall defects ( Li et al, 2020 ). The strain Bacillus sonorensis KLBC GS-3 could inhibit mycelia growth of Penicillium digitatum and conidial germination in vitro ( Deng et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

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Antifungal activity of the volatile organic compounds produced by Ceratocystis fimbriata strains WSJK-1 and Mby

Gao

Ren²,

ShuQi³

et al. 2022

Front. Microbiol.

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Microorganism-produced volatile organic compounds (VOCs) are considered promising environmental-safety fumigants in food preservation. In this study, the VOCs from fungal Ceratocystis fimbriata strains (WSJK-1, Mby) were tested against postharvest fungi Monilinia laxa, Fusarium oxysporum, Monilinia fructicola, Botrytis cinerea, Alternaria solani, and Aspergillus flavus in vitro. The mycelial growth was significantly inhibited, in particular M. fructicola and B. cinerea (76.95, 76.00%), respectively. VOCs were identified by headspace solid-phase microextraction coupled with Gas Chromatography–Mass Spectrometry (HS-SPME-GC–MS); 40 compounds were identified. The antifungal activity of 21 compounds was tested by the minimum inhibitory concentrations (MIC) value. Benzaldehyde, 2-Phenylethanol, and 1-Octen-3-ol showed strong antifungal activity with the MIC in vitro ranging from 0.094 to 0.284 ml L−1 depending on the pathogen tested. The optical microscope showed serious morphological damage, including cell deformation, curling, collapse, and deficiency in mycelial or conidia cell structures treated with C. fimbriata VOCs and pure compounds. In vivo tests, C. fimbriata VOCs decreased brown rot severity in peaches, and compounds Benzaldehyde and 2-Phenylethanol could reduce peach brown rot in peaches at 60 μl L−1. The VOCs produced by C. fimbriata strain have good antifungal effects; low concentration fumigation could control peach brown rot. Its fragrance is fresh, safe, and harmless, and it is possible to replace chemical fumigants. It could be used as a potential biofumigant to control fruit postharvest transportation, storage, and food preservation. To the best of our knowledge, this is the first report on the antifungal activity and biocontrol mechanism of VOCs produced by C. fimbriata.

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Section: Resultsmentioning

confidence: 99%

“…"+ +" represents that the inhibition rate of the compound against pathogens is more than 90% at the concentration of 2.37 ml L −1 . (Andersen, 2006;Wang et al, 2020). Limonene is found in natural fruits like grapefruit, tangerine, orange, mandarine, and lemon.…”

Section: Antifungal Pure Vocs From Ceratocystis Fimbriata Strainmentioning

confidence: 99%

Antifungal activity of the volatile organic compounds produced by Ceratocystis fimbriata strains WSJK-1 and Mby

Gao

Ren²,

ShuQi³

et al. 2022

Front. Microbiol.

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“…These results are consistent with those of Calvo et al (2017) [ 54 ], who reported a strong in vitro antifungal activity of B. amyloliquefaciens BUZ-14 against many postharvest pathogens including M. fructicola , B. cinerea , M. laxa , P. expansum , P. italicum and P. digitatum . Similarly, Deng et al (2020) [ 94 ] reported that B. sonorensis KLBC GS-3 exhibited a significant inhibitory in vitro activity against P. digitatum . The potential of B. amyloliquefaciens as a biocontrol agent of green mold of citrus fruits caused P. digitatum was previously demonstrated by several other researchers [ 74 , 98 ].…”

Section: Discussionmentioning

confidence: 96%

“…Several studies have shown considerable potential of beneficial microorganisms retrieved from diverse agro-systems or natural environments that can be integrated into plant disease management strategies. In particular, while soil is the main source of beneficial bacteria such as Pseudomonas and Bacillus species or filamentous fungi of the genus Trichoderma , widely used for soil bioremediation or as BCAs against soilborne plant diseases [ 88 , 89 , 90 , 91 , 92 , 93 ], fruit surfaces are the most appropriate source of epiphytic, carposphere-competent microbial antagonists able to compete with postharvest fruit pathogens [ 30 , 94 ]. The use of these microbial antagonists as BCAs has the advantage of being a toxicologically and environmentally safe approach to control diseases of fruit crops [ 95 , 96 ].…”

Section: Discussionmentioning

confidence: 99%

Epiphytic Yeasts and Bacteria as Candidate Biocontrol Agents of Green and Blue Molds of Citrus Fruits

Hammami

Oueslati

Smiri

et al. 2022

JoF

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Overall, 180 yeasts and bacteria isolated from the peel of citrus fruits were screened for their in vitro antagonistic activity against Penicillium digitatum and P. italicum, causative agents of green and blue mold of citrus fruits, respectively. Two yeast and three bacterial isolates were selected for their inhibitory activity on mycelium growth. Based on the phylogenetic analysis of 16S rDNA and ITS rDNA sequences, the yeast isolates were identified as Candida oleophila and Debaryomyces hansenii while the bacterial isolates were identified as Bacillus amyloliquefaciens, B. pumilus and B. subtilis. All five selected isolates significantly reduced the incidence of decay incited by P. digitatum and P. italicum on ‘Valencia’ orange and ‘Eureka’ lemon fruits. Moreover, they were effective in preventing natural infections of green and blue mold of fruits stored at 4 °C. Treatments with antagonistic yeasts and bacteria did not negatively affect the quality and shelf life of fruits. The antagonistic efficacy of the five isolates depended on multiple modes of action, including the ability to form biofilms and produce antifungal lipopeptides, lytic enzymes and volatile compounds. The selected isolates are promising as biocontrol agents of postharvest green and blue molds of citrus fruits.

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“…Several preservation methods have been applied to maintain postharvest storability of harvested pummelo fruit, such as CaCl 2 ( Chen et al., 2005 ), chitosan ( Nie et al., 2020 ; Chen et al., 2021 ), gibberellic acid ( Porat et al., 2001 ), and 1-methylcyclopropene ( Lacerna et al., 2018 ). As the closest citrus fruit to pummelo fruit, grapefruit can be effectively preserved by using several postharvest approaches, including hot water, biocontrol agent GS-3, neem leaf extract, and pectic oligosaccharides ( Porat et al., 2000 ; Vera-Guzmán et al., 2019 ; Deng et al., 2020 ; Khan et al., 2021 ). Due to the long-established application of postharvest methods for pummelo, and the dissimilarities between the above two citrus varieties, the development of effective postharvest technologies for pummelo preservation is of great importance and urgency.…”

Section: Introductionmentioning

confidence: 99%

Maintenance of postharvest storability and overall quality of ‘Jinshayou’ pummelo fruit by salicylic acid treatment

Huang

Chen

et al. 2023

Front. Plant Sci.

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IntroductionThe loss of postharvest storability of pummelo fruit reduces its commodity value for long run. To maintain its storability, the effects of postharvest dipping treatment by salicylic acid (SA) with different concentrations (0, 0.1, 0.2, or 0.3%) were investigated on pummelo fruit (Citrus maxima Merr. cv. Jinshayou) during the room temperature storage at 20 ± 2°C for 90 d.Results and discussionAmong all treatments, pre-storage SA treatment at 0.3% demonstrated the most significant ability to reduce fruit decay incidence, decrease weight loss, delay peel color-turned process, and inhibit the declines in total soluble solids (TSS) as well as titratable acid (TA) content. The increases in electrolyte leakage, hydrogen peroxide (H2O2), and malondialdehyde (MDA) content of the 0.3% SA-treated pummelo fruit were reduced compared to the control (dipped in distilled water). Pummelo fruit treated with 0.3% SA exhibited the most outstanding ability to excess reactive oxygen species (ROS) accumulation, as evidenced by promoted the increases in glutathione (GSH), total phenolics and flavonoids contents, delayed the AsA decline, and enhanced the activities of antioxidant enzymes and their encoding genes expression.ConclusionPre-storage treatment dipped with SA, particularly at 0.3%, can be used as a useful and safe preservation method to maintain higher postharvest storability and better overall quality of ‘Jinshayou’ pummelo fruit, and thus delaying postharvest senescence and extend the storage life up to 90 d at room temperature.

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Identification of a new Bacillus sonorensis strain KLBC GS-3 as a biocontrol agent for postharvest green mould in grapefruit

Cited by 23 publications

References 57 publications

Antifungal activity of the volatile organic compounds produced by Ceratocystis fimbriata strains WSJK-1 and Mby

Antifungal activity of the volatile organic compounds produced by Ceratocystis fimbriata strains WSJK-1 and Mby

Epiphytic Yeasts and Bacteria as Candidate Biocontrol Agents of Green and Blue Molds of Citrus Fruits

Maintenance of postharvest storability and overall quality of ‘Jinshayou’ pummelo fruit by salicylic acid treatment

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