Identification of a new adhesin-like protein from Lactobacillus mucosae ME-340 with specific affinity to the human blood group A and B antigens

Abstract

Aims: To identify and characterize a new adhesin‐like protein of probiotics that show specific adhesion to human blood group A and B antigens. Methods and Results: Using the BIACORE assay, the adhesion of cell surface components obtained from four lactobacilli strains that adhered to blood group A and B antigens was tested. Their components showed a significant adhesion to A and B antigens when compared to the bovine serum albumin (BSA) control. The 1 mol l−1 GHCl fraction extracted from Lactobacillus mucosae ME‐340 contained a 29‐kDa band (Lam29) using SDS–PAGE. The N‐terminal amino acid sequence and homology analysis showed that Lam29 was 90% similar to the substrate‐binding protein of the ATP‐binding cassette (ABC) transporter from Lactobacillus fermentum IFO 3956. The complete nucleotide sequence (858 bp) of Lam29 was determined and encoded a protein of 285 amino acid residues. Phylogenetic analysis and multiple sequence alignments indicated this protein may be related to the cysteine‐binding transporter. Conclusions: The adhesion of ME‐340 strain to blood group A and B antigens was mediated by Lam29 that is a putative component of ABC transporter as an adhesin‐like protein. Significance and Impact of the Study: Lactobacillus mucosae ME‐340 expressing Lam29 may be useful for competitive exclusion of pathogens via blood group antigen receptors in the human gastrointestinal mucosa and in the development of new probiotic foods.

Colonic mucosa-associated microbiota is influenced by an interaction of Crohn disease and FUT2 ( Secretor ) genotype

“…Importantly, several of these OTUs are considered to be probiotic [e.g., Lactobacillus (51) and Faecalibacterium (52)] and/or were previously observed to be reduced in the context of CD (e.g., Faecalibacterium prausnitzii). Our observation that OTUs belonging to the Lactobacillus genus are particularly associated with healthy secretors is also consistent with the fact that they possess adhesins specifically targeting ABO blood group antigens in the mucosa (53), which may contribute to the probiotic function of Lactobacillus by blocking the attachment of potential pathogens to mucosal surfaces (54). Interestingly, although Stenotrophomonas is becoming increasingly important as a nosocomial pathogen-associated with airway infections, weak bacteremia in immunocompromised subjects, resistance to antibiotics, and even life-threatening chronic enteritis (55,56)-several species' OTUs belonging to this genus were identified among our controls.…”

An Adhesin-Like Protein, Lam29, fromLactobacillus mucosaeME-340 Binds to Histone H3 and Blood Group Antigens in Human Colonic Mucus

“…12) In the present study, we focused on proteinaceous components other than blood group antigens on human colonic mucus as receptor-like molecules for Lam29.…”

Genome Sequence of Lactobacillus mucosae LM1, Isolated from Piglet Feces

“…actobacillus mucosae, found in the mammalian gastrointestinal tract, has been shown to have the ability to adhere to mucosal surfaces (2,3,9). Previous reports have identified L. mucosae as having the ability to attach tightly to the epithelium of the human intestine and to produce antimicrobials and a biofilm when exposed to the physiological conditions of the gut (4).…”

“…Previous reports have identified L. mucosae as having the ability to attach tightly to the epithelium of the human intestine and to produce antimicrobials and a biofilm when exposed to the physiological conditions of the gut (4). The adhesion of lactobacilli to gastrointestinal mucus makes them a good choice for probiotics since it increases their ability to colonize the gut efficiently, modulate the intestinal immune system, and inhibit pathogenic bacteria (1,4,9).…”

“…Moreover, the L. mucosae LM1 genome includes a putative ABC transporter and adhesin-like protein (LBLM1_10110) with significant homology (100% coverage and 93% similarity) to L. mucosae ABC 2745, which has specific affinity for the human blood group antigens A and B and which protects against the attachment of pathogenic bacteria (9). The mub and adhesin-like protein homologs in the L. mucosae LM1 genome indicate that LM1 may be useful in competitive exclusion against pathogens through blood group antigen receptors in the human gastrointestinal mucosa (9).…”