Identification of a mutarotase gene involved in D-galactose utilization in Aspergillus nidulans

Kulcsár, László; Flipphi, Michel; Jónás, Ágota; Sándor, Erzsébet; Fekete, Erzsébet; Karaffa, Levente

doi:10.1093/femsle/fnx202

Cited by 8 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In line with this hypothesis, LadA (AN0942), associated with the production of L-sorbose, was suggested in a recent study [74]. In our study, we predicted the same gene set involved in this oxidoreductive pathway in A. nidulans, as previously reported [79,80,82]. Although the oxidoreductive pathway for D-galactose catabolism was well established in Aspergillus species and T. reesei, knowledge of the oxidoreductive pathway in Penicillium and Basidiomycota species is scarce.…”

supporting

confidence: 85%

The Sugar Metabolic Model of Aspergillus niger Can Only Be Reliably Transferred to Fungi of Its Phylum

Chroumpi

Garrigues

et al. 2022

JoF

View full text Add to dashboard Cite

Fungi play a critical role in the global carbon cycle by degrading plant polysaccharides to small sugars and metabolizing them as carbon and energy sources. We mapped the well-established sugar metabolic network of Aspergillus niger to five taxonomically distant species (Aspergillus nidulans, Penicillium subrubescens, Trichoderma reesei, Phanerochaete chrysosporium and Dichomitus squalens) using an orthology-based approach. The diversity of sugar metabolism correlates well with the taxonomic distance of the fungi. The pathways are highly conserved between the three studied Eurotiomycetes (A. niger, A. nidulans, P. subrubescens). A higher level of diversity was observed between the T. reesei and A. niger, and even more so for the two Basidiomycetes. These results were confirmed by integrative analysis of transcriptome, proteome and metabolome, as well as growth profiles of the fungi growing on the corresponding sugars. In conclusion, the establishment of sugar pathway models in different fungi revealed the diversity of fungal sugar conversion and provided a valuable resource for the community, which would facilitate rational metabolic engineering of these fungi as microbial cell factories.

show abstract

supporting

confidence: 85%

The Sugar Metabolic Model of Aspergillus niger Can Only Be Reliably Transferred to Fungi of Its Phylum

Chroumpi

Garrigues

et al. 2022

JoF

View full text Add to dashboard Cite

show abstract

“…Recently, we have shown that an intracellular galactose-1-epimerase (GalmB: Locus AN3432; EC 5.1.3.3), which interconverts the αand β-anomers of D-galactose in solution, contributes to the flux through the Leloir pathway (Kulcsár et al, 2017). The last three enzymes of the Leloir pathway have important anabolic functions in the production of cell wall precursors UDP-glucose and UDP-galactose (El-Ganiny et al, 2010).…”

Section: Selection Of Diagnostic Genes Of D-galactose-and L-arabinose Catabolismmentioning

confidence: 99%

l-Arabinose induces d-galactose catabolism via the Leloir pathway in Aspergillus nidulans

Németh

Kulcsár

Flipphi

et al. 2019

Fungal Genetics and Biology

Self Cite

View full text Add to dashboard Cite

L-Arabinose and D-galactose are the principal constituents of L-arabinogalactan, and also cooccur in other hemicelluloses and pectins. In this work we hypothesized that similar to the induction of relevant glycoside hydrolases by monomers liberated from these plant heteropolymers, their respective catabolisms in saprophytic and phytopathogenic fungi may respond to the presence of the other sugar to promote synergistic use of the complex growth substrate. We showed that these two sugars are indeed consumed simultaneously by Aspergillus nidulans, while L-arabinose is utilised faster in the presence than in the absence of D-galactose. Furthermore, the first two genes of the Leloir pathway for D-galactose catabolismencoding D-galactose 1-epimerase and galactokinaseare induced more rapidly by L-arabinose than by D-galactose eventhough deletion mutants thereof grow as well as a wild type strain on the pentose. D-Galactose 1-epimerase is hyperinduced by L-arabinose, Dxylose and L-arabitol but not by xylitol. The results suggest that in A. nidulans, L-arabinose and D-xyloseboth requiring NADPH for their catabolisationactively promote the enzyme infrastructure necessary to convert -D-galactopyranose via the Leloir pathway with its anomer specific enzymes, into -D-glucose-6-phosphate (the starting substrate of the oxidative part of the pentose phosphate pathway) even in the absence of D-galactose.

show abstract

“…Galactose, though a non-preferred carbon source, is usually converted to bioavailable molecules including glucose and fructose derivatives ( Kulcsár et al, 2017 ). UDP-glucose is catalyzed into UDP-galactose and galactose-1-phosphate in the presence of GALT, then the UDP-galactose would be converted into UDP-glucose under the catalysis of GALE ( Schuler et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Integrated metabolomic and transcriptomic analysis reveal the effect of mechanical stress on sugar metabolism in tea leaves (Camellia sinensis) post-harvest

Zhang¹,

Tan²,

Feng³

et al. 2023

PeerJ

View full text Add to dashboard Cite

Sugar metabolites not only act as the key compounds in tea plant response to stress but are also critical for tea quality formation during the post-harvest processing of tea leaves. However, the mechanisms by which sugar metabolites in post-harvest tea leaves respond to mechanical stress are unclear. In this study, we aimed to investigate the effects of mechanical stress on saccharide metabolites and related post-harvest tea genes. Withered (C15) and mechanically-stressed (V15) for 15 min Oolong tea leaves were used for metabolome and transcriptome sequencing analyses. We identified a total of 19 sugar metabolites, most of which increased in C15 and V15. A total of 69 genes related to sugar metabolism were identified using transcriptome analysis, most of which were down-regulated in C15 and V15. To further understand the relationship between the down-regulated genes and sugar metabolites, we analyzed the sucrose and starch, galactose, and glycolysis metabolic pathways, and found that several key genes of invertase (INV), α-amylase (AMY), β-amylase (BMY), aldose 1-epimerase (AEP), and α-galactosidase (AGAL) were down-regulated. This inhibited the hydrolysis of sugars and might have contributed to the enrichment of galactose and D-mannose in V15. Additionally, galactinol synthase (Gols), raffinose synthase (RS), hexokinase (HXK), 6-phosphofructokinase 1 (PFK-1), and pyruvate kinase (PK) genes were significantly upregulated in V15, promoting the accumulation of D-fructose-6-phosphate (D-Fru-6P), D-glucose-6-phosphate (D-glu-6P), and D-glucose. Transcriptome and metabolome association analysis showed that the glycolysis pathway was enhanced and the hydrolysis rate of sugars related to hemicellulose synthesis slowed in response to mechanical stress. In this study, we explored the role of sugar in the response of post-harvest tea leaves to mechanical stress by analyzing differences in the expression of sugar metabolites and related genes. Our results improve the understanding of post-harvest tea’s resistance to mechanical stress and the associated mechanism of sugar metabolism. The resulting treatment may be used to control the quality of Oolong tea.

show abstract

Identification of a mutarotase gene involved in D-galactose utilization in Aspergillus nidulans

Cited by 8 publications

References 31 publications

The Sugar Metabolic Model of Aspergillus niger Can Only Be Reliably Transferred to Fungi of Its Phylum

The Sugar Metabolic Model of Aspergillus niger Can Only Be Reliably Transferred to Fungi of Its Phylum

l-Arabinose induces d-galactose catabolism via the Leloir pathway in Aspergillus nidulans

Integrated metabolomic and transcriptomic analysis reveal the effect of mechanical stress on sugar metabolism in tea leaves (Camellia sinensis) post-harvest

Contact Info

Product

Resources

About